Multicapillary column for chromatography and sample preparation

Abstract

A multicapillary column especially useful for liquid chromatography and sample preparation comprising a plurality of uniform capillaries coated with an insoluble stationary phase, wherein the thickness of the stationary phase is correlated with the radius of the individual capillaries for high efficiency.

1 . A multicapillary column, especially useful for liquid chromatography and sample preparation applications, comprising:
 (a) a plurality of capillary tubes contained within a column for receiving a sample at a first end of the column and discharging a separated sample at a second end of the column;   (b) an insoluble stationary phase being coated directly on interior surfaces of said capillary tubes; said stationary phase coating comprising a thickness that is correlated with the radius of individual capillary tubes for achieving high efficiency capillary tubes by applying a greater amount of stationary phase on wider capillary tubes and a lesser amount of stationary phase on narrower capillary tubes to enhance uniformity of said capillary tubes; and
 wherein the insoluble stationary phase coating additionally contains peptide moieties. 
   
     
     
         2 . A multicapillary column, especially useful for liquid chromatography and sample preparation applications, comprising:
 (a) a plurality of capillary tubes contained within a column for receiving a sample at a first end of the column and discharging a separated sample at a second end of the column;   (b) an insoluble stationary phase being coated directly on interior surfaces of said capillary tubes; said stationary phase coating comprising a thickness that is correlated with the radius of individual capillary tubes for achieving high efficiency capillary tubes by applying a greater amount of stationary phase on wider capillary tubes and a lesser amount of stationary phase on narrower capillary tubes to enhance uniformity of said capillary tubes; and   wherein the stationary phase coating additionally contains macrocyclic glycopeptide moieties.   
     
     
         3 . A multicapillary column, especially useful for liquid chromatography and sample preparation applications, comprising:
 (a) a plurality of capillary tubes contained within a column for receiving a sample at a first end of the column and discharging a separated sample at a second end of the column;   (b) an insoluble stationary phase being coated directly on interior surfaces of said capillary tubes; said stationary phase coating comprising a thickness that is correlated with the radius of individual capillary tubes for achieving high efficiency capillary tubes by applying a greater amount of stationary phase on wider capillary tubes and a lesser amount of stationary phase on narrower capillary tubes to enhance uniformity of said capillary tubes; and   wherein the stationary phase coating additionally contains protein moieties.   
     
     
         4 . A multicapillary column, especially useful for liquid chromatography and sample preparation applications, comprising:
 (a) a plurality of capillary tubes contained within a column for receiving a sample at a first end of the column and discharging a separated sample at a second end of the column;   (b) an insoluble stationary phase being coated directly on interior surfaces of said capillary tubes; said stationary phase coating comprising a thickness that is correlated with the radius of individual capillary tubes for achieving high efficiency capillary tubes by applying a greater amount of stationary phase on wider capillary tubes and a lesser amount of stationary phase on narrower capillary tubes to enhance uniformity of said capillary tubes; and   wherein the stationary phase coating additionally contains antibody moieties.