Method for Producing the Flu Virus

Abstract

The invention relates to a method for producing flu virus according to which: a) immunizing a hen by administering a flu vaccine to the hen, b) triggering embryogenesis in one or more eggs of the immunized hen, c) infecting the one or more embryonated eggs by inoculating a flu virus into the allantoic cavity of the eggs, d) incubating the one or more infected embryonated eggs under temperature and humidity conditions that allow replication of the virus, and e) harvesting the allantoic fluid of the one or more incubated eggs containing the virus.

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         20 . A flu vaccine composition which comprises a flu virus or a flu virus-derived product obtained by
 a) immunizing a hen by administering a flu vaccine to the hen,   b) triggering embryogenesis in one or more eggs of the immunized hen,   c) infecting the one or more embryonated eggs by inoculating a flu virus into the allantoic cavity of the eggs,   d) incubating the one or more infected embryonated eggs under temperature and humidity conditions that allow replication of the virus,   e) harvesting the allantoic fluid of the one or more incubated eggs containing the virus,   f) purifying the virus, and   g) inactivating the virus.   
     
     
         21 . A method for preventing infection against an epidemic or a pandemic flu strain in a human subject, which method comprises administering to the human subject an effective amount of a flu vaccine composition as claimed in  claim 20 . 
     
     
         22 . A method for preventing flu in an animal selected from the group of equine family, porcine family, canine family, feline family, mustelids and avian species, which method comprises administering to the animal an effective amount of a flu vaccine composition as claimed in  claim 20 .