Ophthalmic Surgical Irrigating Solutions Containing Hyaluronidase

Abstract

An ophthalmic irrigating solution containing hyaluronidase and an antioxidant is provided. The solution prevents a post-operative intraocular pressure rise when used during an ophthalmic surgical procedure, and also protects corneal endothelial cells. A method for preventing post-operative intraocular pressure increases in an eye during ophthalmic surgery involves irrigating an anterior chamber of the eye with an ophthalmic solution containing hyaluronidase and an antioxidant. Kits containing hyaluronidase and base medium solutions are also provided. The kits are designed such that the hyaluronidase and base medium solution are combined and administered together to an eye of a patient during or following an ophthalmic surgical procedure, such as cataract surgery, intraocular lens surgery, corneal transplant surgery, or glaucoma surgery. Administration of the components of the kit prevents an increase in post-operative intraocular pressure and also protects corneal endothelial cells.

1 . An ophthalmic irrigating solution comprising hyaluronidase and an antioxidant, wherein the solution prevents a post-operative intraocular pressure rise when used during an ophthalmic surgical procedure. 
     
     
         2 . The solution according to  claim 1 , wherein the ophthalmic surgical procedure is selected from the group consisting of cataract surgery, intraocular lens surgery, corneal transplant surgery, and glaucoma surgery. 
     
     
         3 . The solution according to  claim 1 , further comprising a base medium. 
     
     
         4 . The solution according to  claim 3 , wherein the base medium comprises at least one inorganic salt selected from the group consisting of calcium chloride, magnesium chloride, magnesium sulfate, potassium chloride, potassium phosphate, sodium bicarbonate, sodium chloride, and sodium phosphate. 
     
     
         5 . The solution according to  claim 1 , wherein the antioxidant is selected from the group consisting of ascorbic acid, capsaicin, fisetin, gallic acid, hydralazine, morin, quercetin, trolox, and glutathione. 
     
     
         6 . The solution according to  claim 1 , further comprising at least one energy source. 
     
     
         7 . The solution according to  claim 6 , wherein the energy source is selected from the group consisting of dextrose and β-hydroxybutyrate. 
     
     
         8 . The solution according to  claim 1 , further comprising at least one component selected from the group consisting of ATP and precursors thereof. 
     
     
         9 . The solution according to  claim 1 , further comprising at least one component for maintaining cell density and/or cell viability. 
     
     
         10 . The solution according to  claim 9 , wherein the component for maintaining cell density and/or cell viability is high molecular weight dextran. 
     
     
         11 . The solution according to  claim 1 , wherein a pH of the solution is about 6.8 to about 9. 
     
     
         12 . The solution according to  claim 11 , wherein the pH is about 6.8 to about 8.2. 
     
     
         13 . The solution according to  claim 1 , wherein an osmolality of the solution is about 300 to about 350 mOsm. 
     
     
         14 . The solution according to  claim 1 , wherein the solution comprises about 0.1 to about 25 units hyaluronidase per ml of solution.