Nucleoside-containing compositions and methods for treating red blood cells

Abstract

Blood storage and/or rejuvenating compositions that include D-ribose and a nucleoside other than inosine (e.g., guanosine) are disclosed herein. Such compositions can be useful in methods for treating (e.g., storing and/or rejuvenating) red blood cells.

1 . A blood storage and/or rejuvenating composition comprising a nucleoside and D-ribose, with the proviso that the nucleoside is not inosine. 
     
     
         2 . The composition of  claim 1  wherein the nucleoside is selected from the group consisting of adenosine, deoxyadenosine, guanosine, deoxyguanosine, 5′-methyluridine, thymidine, uridine, deoxyuridine, cytidine, and deoxycytidine. 
     
     
         3 . The composition of  claim 1  wherein the nucleoside is guanosine. 
     
     
         4 . The composition of  claim 1  further comprising sodium pyruvate. 
     
     
         5 . The composition of  claim 1  further comprising an inorganic phosphate. 
     
     
         6 . The composition of  claim 1  wherein the composition is an aqueous solution. 
     
     
         7 . A blood storage and/or rejuvenating composition comprising 75 to 1500 mM guanosine. 
     
     
         8 . The composition of  claim 7  further comprising D-ribose. 
     
     
         9 . The composition of  claim 8  wherein the concentration of the D-ribose is 75 to 1500 mM. 
     
     
         10 . The composition of  claim 7  further comprising sodium pyruvate. 
     
     
         11 . The composition of  claim 10  wherein the concentration of the sodium pyruvate is 75 to 1500 mM. 
     
     
         12 . The composition of  claim 7  further comprising an inorganic phosphate. 
     
     
         13 . The composition of  claim 12  wherein the concentration of the inorganic phosphate is 75 to 1500 mM. 
     
     
         14 . The composition of  claim 7  further comprising inosine. 
     
     
         15 . The composition of  claim 7  further comprising L-arginine. 
     
     
         16 . The composition of  claim 15  wherein the concentration of the L-arginine is 75 to 1500 mM. 
     
     
         17 . A blood storage and/or rejuvenating composition comprising:
 225 mM guanosine;   300 mM D-ribose;   300 mM sodium pyruvate; and   300 mM inorganic phosphate.   
     
     
         18 . A method of storing blood, the method comprising contacting red blood cells with a blood storage and/or rejuvenating composition according to  claim 1 . 
     
     
         19 . The method of  claim 18  wherein the red blood cells are packed red blood cells or in whole blood. 
     
     
         20 . A method of rejuvenating blood, the method comprising contacting red blood cells with a blood storage and/or rejuvenating composition according to  claim 1 . 
     
     
         21 . The method of  claim 20  wherein the red blood cells are packed red blood cells or in whole blood. 
     
     
         22 . A method of rejuvenating blood, the method comprising:
 providing red blood cells having a 2,3-diphosphoglycerate value lower than the value for freshly drawn blood; and   mixing the red blood cells with a blood storage and/or rejuvenating composition under conditions effective to increase the 2,3-diphosphoglycerate value,   wherein the blood storage and/or rejuvenating composition comprises guanosine.   
     
     
         23 . The method of  claim 22  wherein conditions effective comprise incubating the cells in the blood storage and/or rejuvenating composition at a temperature of 4° C. to 37° C. 
     
     
         24 . The method of  claim 23  wherein the temperature is room temperature. 
     
     
         25 . The method of  claim 22  wherein conditions effective comprise incubating the cells in the blood storage and/or rejuvenating composition for a time of at least 10 minutes. 
     
     
         26 . The method of  claim 25  wherein the time is 10 minutes to 48 hours. 
     
     
         27 . The method of  claim 26  wherein the time is 10 minutes to 4 hours. 
     
     
         28 . The method of  claim 27  wherein the time is 30 minutes to 2 hours. 
     
     
         29 . The method of  claim 22  wherein the blood storage and/or rejuvenating composition further comprises D-ribose. 
     
     
         30 . The method of  claim 22  wherein the red blood cells are packed red blood cells or in whole blood. 
     
     
         31 . A method of rejuvenating blood, the method comprising:
 providing red blood cells having an adenosine triphosphate value lower than the value for freshly drawn blood; and   mixing the red blood cells with a blood storage and/or rejuvenating composition under conditions effective to increase the adenosine triphosphate value,   wherein the blood storage and/or rejuvenating composition comprises guanosine.