US2011262475A1PendingUtilityA1
Lyme disease vaccine

Assignee: EARNHART CHRISTOPHER GPriority: May 2, 2008Filed: May 1, 2009Published: Oct 27, 2011
Est. expiryMay 2, 2028(~1.8 yrs left)· nominal 20-yr term from priority
Inventors:Christopher Earnhart Richard T. Marconi
G01N 33/56911C07K 14/001C07K 16/1207C07K 14/20G01N 2469/20A61P 37/04G01N 2333/20C07K 2319/21A61K 39/0225C07K 2319/00A61P 31/04A61K 39/00Y02A50/30
PatentIndex Score
Cited by
References
Claims
Abstract

Antigenic polypeptides comprising linear immunodominant epitopes of Borrelia outer surface protein A (OspA) or Borrelia outer surface protein C (OspC) are useful as vaccines against Lyme disease, and as diagnostics for detecting Borrelia infections. The OspA and OspC antigenic polypeptides typically comprise a plurality of peptides representing epitope containing regions from multiple distinct phyletic groups. The antigenic polypeptides may also include epitopes from both Borrelia OspA and Borrelia OspC.
Claims

exact text as granted — not AI-modified
1 . An isolated recombinant or synthetic peptide or polypeptide comprising at least one linear epitope from  Borrelia  outer surface protein A (OspA), said at least one linear epitope having an amino acid sequence selected from the group consisting of: 
       
         
           
                 
               
                   (SEQ ID NO: 1) 
                 
                   STLTITVNSKKTKDLVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 2) 
                 
                   STLTIIVDSKNKTKLVFTKQ 
                 
                     
                 
                   (SEQ ID NO: 3) 
                 
                   STLTISKNRTKTKQLVFTKE 
                 
                     
                 
                   (SEQ ID NO: 4) 
                 
                   STLTISANSKKTKDLVFLTN 
                 
                     
                 
                   (SEQ ID NO: 5) 
                 
                   STLTITVNNKKTKALVFTKQ 
                 
                     
                 
                   (SEQ ID NO: 6) 
                 
                   STLTISVNSKKTTQLVFTKQ 
                 
                     
                 
                   (SEQ ID NO: 7) 
                 
                   STLTISVNSKKTKNIVFTKE 
                 
                     
                 
                   (SEQ ID NO: 8) 
                 
                   STLTISVNSQKTKNLVFTKE 
                 
                     
                 
                   (SEQ ID NO: 9) 
                 
                   NTLTVSADSKKIKDFVFLTD 
                 
                     
                 
                   (SEQ ID NO: 10) 
                 
                   STLTISKNSQKTKQLVFTKE 
                 
                     
                 
                   (SEQ ID NO: 11) 
                 
                   STLTISAKNKKTKDLVFTKQ 
                 
                     
                 
                   (SEQ ID NO: 12) 
                 
                   STLKISKNSKKTKQLVFTKE 
                 
                     
                 
                   (SEQ ID NO: 13) 
                 
                   STLTISAKSKKTKDLVFTKQ 
                 
                     
                 
                   (SEQ ID NO: 14) 
                 
                   STLTISANNKKTKDLVFTKQ 
                 
                     
                 
                   (SEQ ID NO: 15) 
                 
                   KTLTVSADSKKIKDFVFLTD 
                 
                     
                 
                   (SEQ ID NO: 16) 
                 
                   STLTISAKNKKTTDLVFTKQ 
                 
                     
                 
                   (SEQ ID NO: 17) 
                 
                   STLTISKNSRKTKQLVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 18) 
                 
                   STLTISVNSRKTKNLVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 19) 
                 
                   CKQNVSSLDEKNSVSVDLPGEMKVLVSKEKNKDGKYDLIATVDKLELK 
                 
                     
                 
                   GTS; 
                 
                     
                 
                   (SEQ ID NO: 20) 
                 
                   CKQNVSSLDEKNSVSVDLPGGMTVLVSKEKDKDGKYSLEATVDKLELK 
                 
                     
                 
                   GTS; 
                 
                     
                 
                   (SEQ ID NO: 21) 
                 
                   CKQNVSSLDEKNSASVDLPGEMKVLVSKEKDKDGKYSLKATVDKIELK 
                 
                     
                 
                   GTS; 
                 
                     
                 
                   (SEQ ID NO: 22) 
                 
                   LGQTTLEVFKEDGKTLVSKKVTSKDKSSTEEKFNEKGEVSEKLITRAD 
                 
                     
                 
                   GTR; 
                 
                     
                 
                   (SEQ ID NO: 23) 
                 
                   LSQTKFEIFKEDGKTLVSKKVTLKDKSSTEEKFNEKGETSEKTIVRAN 
                 
                     
                 
                   GTR; 
                 
                     
                 
                   (SEQ ID NO: 24) 
                 
                   LSKPTFELFKGDGETLVSRKVSSKDKTSTDEMFNEKGELSAKTMTREN 
                 
                     
                 
                   GTK; 
                 
                     
                 
                   (SEQ ID NO: 25) 
                 
                   KSSTEEKFNEKGEVSEKIITRADGTRLEYTGIKSDSGKAKEVLKGYVL 
                 
                     
                 
                   EG; 
                 
                     
                 
                   (SEQ ID NO: 26) 
                 
                   KSSTEEKFNEKGETSEKTIVRANGTRLEYTDIKSDGSGKAKEVLKDFT 
                 
                     
                 
                   LEG; 
                 
                   and 
                 
                     
                 
                   (SEQ ID NO: 27) 
                 
                   KTSTDEMFNEKGELSAKTMTRENGTKLEYTEMKSDGTGKAKEVLKNFT 
                 
                     
                 
                   LEG. 
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         2 . The isolated recombinant or synthetic peptide or polypeptide of  claim 1 , wherein said at least one linear epitope is selected from the group consisting of 
       
         
           
                 
                 
                 
               
                     
                   STLTITVNSKKTKDLVFTKE; 
                   (SEQ ID NO: 1) 
                 
                     
                     
                 
                     
                   STLTIIVDSKNKTKLVFTKQ; 
                   (SEQ 1D NO: 2) 
                 
                     
                     
                 
                     
                   STLTISKNRTKTKQLVFTKE; 
                   (SEQ ID NO: 3) 
                 
                     
                     
                 
                     
                   STLTISANSKKTKDLVFLTN; 
                   (SEQ ID NO: 4) 
                 
                     
                     
                 
                     
                   STLTITVNNKKTKALVFTKQ; 
                   (SEQ ID NO: 5) 
                 
                     
                     
                 
                     
                   STLTISVNSKKTTQLVFTKQ; 
                   (SEQ ID NO: 6) 
                 
                     
                     
                 
                     
                   STLTISVNSKKTKNIVFTKE; 
                   (SEQ ID NO: 7) 
                 
                     
                     
                 
                     
                   STLTISVNSQKTKNLVFTKE; 
                   (SEQ ID NO: 8) 
                 
                     
                     
                 
                     
                   NTLTVSADSKKIKDFVFLTD; 
                   (SEQ ID NO: 9) 
                 
                     
                     
                 
                     
                   STLTISKNSQKTKQLVFTKE; 
                   (SEQ ID NO: 10) 
                 
                     
                     
                 
                     
                   STLTISAKNKKTKDLVFTKQ; 
                   (SEQ ID NO: 11) 
                 
                     
                     
                 
                     
                   STLKISKNSKKTKQLVFTKE; 
                   (SEQ ID NO: 12) 
                 
                     
                     
                 
                     
                   STLTISAKSKKTKDLVFTKQ; 
                   (SEQ ID NO: 13) 
                 
                     
                     
                 
                     
                   STLTISANNKKTKDLVFTKQ; 
                   (SEQ ID NO: 14) 
                 
                     
                     
                 
                     
                   KTLTVSADSKKIKDFVFLTD; 
                   (SEQ ID NO: 15) 
                 
                     
                     
                 
                     
                   STLTISAKNKKTTDLVFTKQ; 
                   (SEQ ID NO: 16) 
                 
                     
                     
                 
                     
                   STLTISKNSRKTKQLVFTKE; 
                   (SEQ ID NO: 17) 
                 
                     
                   and 
                     
                 
                     
                     
                 
                     
                   STLTISVNSRKTKNLVFTKE. 
                   (SEQ ID NO: 18) 
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         3 . The isolated recombinant or synthetic peptide or polypeptide of  claim 1 , further comprising one or more amino acid sequences that are epitopes of  Borrelia  outer surface protein C (OspC). 
     
     
         4 . The isolated recombinant or synthetic peptide or polypeptide of  claim 3 , wherein said one or more amino acid sequences that are epitopes of  Borrelia  OspC are from an OspC loop 5 region or an OspC alpha helix 5 region, or both. 
     
     
         5 . The isolated recombinant or synthetic peptide or polypeptide of  claim 3 , wherein said one or more amino acid sequences that are epitopes of OspC are from OspC types selected from the group consisting of Smar, PLi, H13, PFiM, SL10, PMit, PKi, Pbes, HT22, Pko, PLj7, VS461, DK15, HT25, A,72a, F, E, M, D, U, I, L, H, Szid, PHez, PWa, B, K, N, and C. 
     
     
         6 . The isolated recombinant or synthetic peptide or polypeptide of  claim 3 , wherein said one or more amino acid sequences that are epitopes of  Borrelia  OspC are selected from the group of polypeptides having sequences as set forth in SEQ ID NOS: 65-73. 
     
     
         7 . A method for eliciting an immune response against  Borrelia  in an individual in need thereof, comprising the step of
 administering to said individual an isolated recombinant or synthetic peptide or polypeptide comprising at least one linear epitope from  Borrelia  outer surface protein A (OspA), said at least one linear epitope having an amino acid sequence selected from the group consisting of:   
       
         
           
                 
               
                   (SEQ ID NO: 1) 
                 
                   STLTITVNSKKTKDLVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 2) 
                 
                   STLTIIVDSKNKTKLVFTKQ; 
                 
                     
                 
                   (SEQ ID NO: 3) 
                 
                   STLTISKNRTKTKQLVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 4) 
                 
                   STLTISANSKKTKDLVFLTN; 
                 
                     
                 
                   (SEQ ID NO: 5) 
                 
                   STLTITVNNKKTKALVFTKQ; 
                 
                     
                 
                   (SEQ ID NO: 6) 
                 
                   STLTISVNSKKTTQLVFTKQ; 
                 
                     
                 
                   (SEQ ID NO: 7) 
                 
                   STLTISVNSKKTKNIVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 8) 
                 
                   STLTISVNSQKTKNLVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 9) 
                 
                   NTLTVSADSKKIKDFVFLTD; 
                 
                     
                 
                   (SEQ ID NO: 10) 
                 
                   STLTISKNSQKTKQLVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 11) 
                 
                   STLTISAKSKKTKDLVFTKQ; 
                 
                     
                 
                   (SEQ ID NO: 12) 
                 
                   STLKISKNSKKTKQLVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 13) 
                 
                   STLTISAKSKKTKDLVFTKQ; 
                 
                     
                 
                   (SEQ ID NO: 14) 
                 
                   STLTISANNKKTKDLVFTKQ; 
                 
                     
                 
                   (SEQ ID NO: 15) 
                 
                   KTLTVSADSKKIKDFVFLTD; 
                 
                     
                 
                   (SEQ ID NO: 16) 
                 
                   STLTISAKNKKTTDLVFTKQ; 
                 
                     
                 
                   (SEQ ID NO: 17) 
                 
                   STLTISKNSRKTKQLVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 18) 
                 
                   STLTISVNSRKTKNLVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 19) 
                 
                   CKQNVSSLDEKNSVSVDLPGEMKVLVSKEKNKDGKYDLIATVDKLELK 
                 
                     
                 
                   GTS; 
                 
                     
                 
                   (SEQ ID NO: 20) 
                 
                   CKQNVSSLDEKNSVSVDLPGGMTVLVSKEKDKDGKYSLEATVDKLELK 
                 
                     
                 
                   GTS; 
                 
                     
                 
                   (SEQ ID NO: 21) 
                 
                   CKQNVSSLDEKNSASVDLPGEMKVLVSKEKDKDGKYSLKATVDKIELK 
                 
                     
                 
                   GTS; 
                 
                     
                 
                   (SEQ ID NO: 22) 
                 
                   LGQTTLEVFKEDGKTLVSKKVTSKDKSSTEEKFNEKGEVSEKIITRAD 
                 
                     
                 
                   GTR; 
                 
                     
                 
                   (SEQ ID NO: 23) 
                 
                   LSQTKFEIFKEDGKTLVSKKVTLKDKSSTEEKENEKGETSEKTIVRAN 
                 
                     
                 
                   GTR; 
                 
                     
                 
                   (SEQ ID NO: 24) 
                 
                   LSKPTFELFKGDGETLVSRKVSSKDKTSTDEMFNEKGELSAKTMTREN 
                 
                     
                 
                   GTK; 
                 
                     
                 
                   (SEQ ID NO: 25) 
                 
                   KSSTEEKFNEKGEVSEKIITRADGTRLEYTGIKSDGSGKAKEVLKGYV 
                 
                     
                 
                   LEG; 
                 
                     
                 
                   (SEQ ID NO: 26)  
                 
                   KSSTEEKFNEKGETSEKTIVRANGTRLEYTDIKSDGSGKAKEVLKDFT 
                 
                     
                 
                   LEG; 
                 
                   and 
                 
                     
                 
                   (SEQ ID NO: 27) 
                 
                   KTSTDEMFNEKGELSAKTMTRENGTKLEYTEMKSDGTGKAKEVLKNFT 
                 
                     
                 
                   LEG. 
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         8 . The method of  claim 7 , wherein said at least one linear epitope has an amino acid sequence selected from the group consisting of 
       
         
           
                 
                 
                 
               
                     
                   STLTITVNSKKTKDLVFTKE; 
                   (SEQ ID NO: 1) 
                 
                     
                     
                 
                     
                   STLTIIVDSKNKTKLVFTKQ; 
                   (SEQ ID NO: 2) 
                 
                     
                     
                 
                     
                   STLTISKNRTKTKQLVFTKE; 
                   (SEQ ID NO: 3) 
                 
                     
                     
                 
                     
                   STLTISANSKKTKDLVFLTN; 
                   (SEQ ID NO: 4) 
                 
                     
                     
                 
                     
                   STLTITVNNKKTKALVFTKQ; 
                   (SEQ ID NO: 5) 
                 
                     
                     
                 
                     
                   STLTISVNSKKTTQLVFIKQ; 
                   (SEQ ID NO: 6) 
                 
                     
                     
                 
                     
                   STLTISVNSKKTKNIVFTKE; 
                   (SEQ ID NO: 7) 
                 
                     
                     
                 
                     
                   STLTISVNSQKTKNLVFTKE; 
                   (SEQ ID NO: 8) 
                 
                     
                     
                 
                     
                   NTLTVSADSKKIKDFVFLTD; 
                   (SEQ ID NO: 9) 
                 
                     
                     
                 
                     
                   STLTISKNSQKTKQLVFTKE; 
                   (SEQ ID NO: 10) 
                 
                     
                     
                 
                     
                   STLTISAKSKKTKDLVFTKQ; 
                   (SEQ ID NO: 11) 
                 
                     
                     
                 
                     
                   STLKISKNSKKTKQLVFTKE; 
                   (SEQ ID NO: 12) 
                 
                     
                     
                 
                     
                   STLTISAKSKKTKDLVFTKQ; 
                   (SEQ ID NO: 13) 
                 
                     
                     
                 
                     
                   STLTISANNKKTKDLVFIKQ; 
                   (SEQ ID NO: 14) 
                 
                     
                     
                 
                     
                   KTLTVSADSKKIKDFVFLTD; 
                   (SEQ ID NO: 15) 
                 
                     
                     
                 
                     
                   STLTISAKNKKTTDLVFTKQ; 
                   (SEQ ID NO: 16) 
                 
                     
                     
                 
                     
                   STLTISKNSRKTKQLVFTKE; 
                   (SEQ ID NO: 17) 
                 
                     
                   and 
                     
                 
                     
                     
                 
                     
                   STLTISVNSRKTKNLVFTKE. 
                   (SEQ ID NO: 18) 
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         9 . The method of  claim 7 , wherein said isolated recombinant or synthetic peptide or polypeptide further comprises one or more epitopes of  Borrelia  outer surface protein C (OspC). 
     
     
         10 . The method of  claim 9 , wherein said one or more amino acid sequences that are epitopes of  Borrelia  OspC are selected from the group of polypeptides having sequences as set forth in SEQ ID NOS: 65-73. 
     
     
         11 . A method for ascertaining whether an individual has been exposed to or infected with  Borrelia , or both, comprising the steps of
 obtaining a biological sample from said individual,   exposing said biological sample to a recombinant or synthetic peptide or polypeptide comprising at least one linear epitope from  Borrelia  outer surface protein A (OspA), said at least one linear epitope having an amino acid sequence selected from the group consisting of:   
       
         
           
                 
               
                   (SEQ ID NO: 1) 
                 
                   STLTITVNSKKTKDLVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 2) 
                 
                   STLTIIVDSKNKTKLVFTKQ; 
                 
                     
                 
                   (SEQ ID NO: 3) 
                 
                   STLTISKNRTKTKQLVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 4) 
                 
                   STLTISANSKKTKDLVFLTN; 
                 
                     
                 
                   (SEQ ID NO: 5) 
                 
                   STLTITVNNKKTKALVFTKQ; 
                 
                     
                 
                   (SEQ ID NO: 6) 
                 
                   STLTISVNSKKTTQLVFTKQ; 
                 
                     
                 
                   (SEQ ID NO: 7) 
                 
                   STLTISVNSKKTKNIVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 8) 
                 
                   STLTISVNSQKTKNLVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 9) 
                 
                   NTLTVSADSKKIKDFVFLTD; 
                 
                     
                 
                   (SEQ ID NO: 10) 
                 
                   STLTISKNSQKTKQLVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 11) 
                 
                   STLTISAKNKKTKDLVFTKQ; 
                 
                     
                 
                   (SEQ ID NO: 12) 
                 
                   STLKISKNSKKTKQLVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 13) 
                 
                   STLTISAKSKKTKDLVFTKQ; 
                 
                     
                 
                   (SEQ ID NO: 14) 
                 
                   STLTISANNKKTKDLVFTKQ; 
                 
                     
                 
                   (SEQ ID NO: 15) 
                 
                   KTLTVSADSKKIKDFVFLTD; 
                 
                     
                 
                   (SEQ ID NO: 16) 
                 
                   STLTISAKNKKTTDLVFTKQ; 
                 
                     
                 
                   (SEQ ID NO: 17) 
                 
                   STLTISKNSRKTKQLVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 18) 
                 
                   STLTISVNSRKTKNLVFTKE; 
                 
                     
                 
                   (SEQ 1D NO: 19) 
                 
                   CKQNVSSLDEKNSVSVDLPGEMKVLVSKEKNKDGKYDLIATVDKLELK 
                 
                     
                 
                   GTS; 
                 
                     
                 
                   (SEQ ID NO: 20) 
                 
                   CKQNVSSLDEKNSVSVDLPGGMTVLVSKEKDKDGKYSLEATVDKLELK 
                 
                     
                 
                   GTS; 
                 
                     
                 
                   (SEQ ID NO: 21) 
                 
                   CKQNVSSLDEKNSASVDLPGEMKVLVSKEKDKDGKYSLKATVDKIELK 
                 
                     
                 
                   GTS; 
                 
                     
                 
                   (SEQ ID NO: 22) 
                 
                   LGQTTLEVFKEDGKTLVSKKVTSKDKSSTEEKFNEKGEVSEKIITRAD 
                 
                     
                 
                   GTR; 
                 
                     
                 
                   (SEQ ID NO: 23) 
                 
                   LSQTKFEIFKEDGKTLVSKKVTLKDKSSTEEKFNEKGETSEKTIVRAN 
                 
                     
                 
                   GTR; 
                 
                     
                 
                   (SEQ ID NO: 24) 
                 
                   LSKPTFELFKGDGETLVSRKVSSKDKTSTDEMFNEKGELSAKTMTREN 
                 
                     
                 
                   GTK; 
                 
                     
                 
                   (SEQ ID NO: 25) 
                 
                   KSSTEEKFNEKGEVSEKIITRADGTRLEYTG1KSDGSGKAKEVLKGYV 
                 
                     
                 
                   LEG; 
                 
                     
                 
                   (SEQ ID NO: 26) 
                 
                   KSSTEEKFNEKGETSEKTIVRANGTRLEYTDIKSDGSGKAKEVLKDFT 
                 
                     
                 
                   LEG; 
                 
                   and 
                 
                     
                 
                   (SEQ ID NO: 27) 
                 
                   KTSTDEMFNEKGELSAKTMTRENGTKLEYTEMKSDGTGKAKEVLKNFT 
                 
                     
                 
                   LEG. 
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
       and
 determining whether antibodies in said biological sample bind to said recombinant or synthetic peptide or polypeptide, wherein detection of antibody binding is indicative of prior exposure to or infection with  Borrelia.    
 
     
     
         12 . The method of  claim 11 , wherein said recombinant or synthetic peptide or polypeptide further comprises one or more epitopes of  Borrelia  outer surface protein C (OspC). 
     
     
         13 . The method of  claim 12 , wherein said one or more amino acid sequences that are epitopes of  Borrelia  OspC are selected from the group of polypeptides having sequences as set forth in SEQ ID NOS: 65-73. 
     
     
         14 . An antibody to a recombinant or synthetic peptide or polypeptide comprising at least one linear epitope from  Borrelia  outer surface protein A (OspA), said at least one linear epitope having an amino acid sequence selected from the group consisting of: 
       
         
           
                 
               
                   (SEQ ID NO: 1) 
                 
                   STLTITVNSKKTKDLVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 2) 
                 
                   STLTIIVDSKNKTKLVFTKQ; 
                 
                     
                 
                   (SEQ ID NO: 3) 
                 
                   STLTISKNRTKTKQLVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 4) 
                 
                   STLTISANSKKTKDLVFLTN; 
                 
                     
                 
                   (SEQ ID NO: 5) 
                 
                   STLTITVNNKKTKALVFTKQ; 
                 
                     
                 
                   (SEQ ID NO: 6) 
                 
                   STLTISVNSKKITQLVFTKQ; 
                 
                     
                 
                   (SEQ ID NO: 7) 
                 
                   STLTISVNSKKTKNIVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 8) 
                 
                   STLTISVNSQKTKNLVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 9) 
                 
                   NTLTVSADSKKIKDFVFLTD; 
                 
                     
                 
                   (SEQ ID NO: 10) 
                 
                   STLTISKNSQKTKQLVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 11) 
                 
                   STLTISAKNKKTKDLVFTKQ; 
                 
                     
                 
                   (SEQ ID NO: 12) 
                 
                   STLKISKNSKKTKQLVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 13) 
                 
                   STLTISAKSKKTKDLVFTKQ; 
                 
                     
                 
                   (SEQ ID NO: 14) 
                 
                   STLTISAKNKKTKDLVFTKQ; 
                 
                     
                 
                   (SEQ ID NO: 15) 
                 
                   KTLTVSADSKKIKDFVFLTD; 
                 
                     
                 
                   (SEQ ID NO: 16) 
                 
                   STLTISAKNKKTTDLVFTKQ; 
                 
                     
                 
                   (SEQ ID NO: 17) 
                 
                   STLTISKNSRKTKQLVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 18) 
                 
                   STLTISVNSRKTKNLVFTKE; 
                 
                     
                 
                   (SEQ ID NO: 19) 
                 
                   CKQNVSSLDEKNSVSVDLPGEMKVLVSKEKNKDGKYDLIATVDKLELK 
                 
                     
                 
                   GTS; 
                 
                     
                 
                   (SEQ ID NO: 20) 
                 
                   CKQNVSSLDEKNSVSVDLPGGMTVLVSKEKDKDGKYSLEATVDKLELK 
                 
                     
                 
                   GTS; 
                 
                     
                 
                   (SEQ ID NO: 21) 
                 
                   CKQNVSSLDEKNSASVDLPGEMKVLVSKEKDKDGKYSLKATVDKIELK 
                 
                     
                 
                   GTS; 
                 
                     
                 
                   (SEQ ID NO: 22) 
                 
                   LGQTTLEVFKEDGKTLVSKKVTSKDKSSTEEKFNEKGEVSEKIITRAD 
                 
                     
                 
                   GTR; 
                 
                     
                 
                   (SEQ ID NO: 23) 
                 
                   LSQTKFETFREDGKTLVSKKVTLKDKSSTEEKFNEKGETSEKTIVRAN 
                 
                     
                 
                   GTR; 
                 
                     
                 
                   (SEQ ID NO: 24) 
                 
                   LSKPTFELFKGDGETLVSRKVSSKDKTSTDEMFNEKGELSAKTMTREN 
                 
                     
                 
                   GTK; 
                 
                     
                 
                   (SEQ ID NO: 25) 
                 
                   KSSTEEKFNEKGEVSEKIITRADGTRLEYTGIKSDGSGKAKEVLKGYV 
                 
                     
                 
                   LEG; 
                 
                     
                 
                   (SEQ ID NO: 26) 
                 
                   KSSTEEKFNEKGETSEKTIVRANGTRLEYTDIKSDGSGKAKEVLKDFT 
                 
                     
                 
                   LEG; 
                 
                   and 
                 
                     
                 
                   (SEQ ID NO: 27) 
                 
                   KTSTDEMFNEKGELSAKTMTRENGTKLEYTEMKSDGTGKAKEVLKNFT 
                 
                     
                 
                   LEG. 
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         15 . The antibody of  claim 19 , wherein said antibody is bactericidal for  Borrelia  spirochetes. 
     
     
         16 . An isolated recombinant or synthetic peptide or polypeptide comprising one or more epitopes from  Borrelia  outer surface protein A (OspA), said one or more epitopes being from an antigenic region selected from the group consisting of: antigenic region 221-240, antigenic region 17-67, antigenic region 94-144, and antigenic region 119-169. 
     
     
         17 . The isolated recombinant or synthetic peptide or polypeptide of  claim 16 , further comprising one or more epitopes of  Borrelia  outer surface protein C (OspC). 
     
     
         18 . An isolated recombinant or synthetic peptide or polypeptide comprising at least one linear epitope from  Borrelia  outer surface protein C (OspC), said at least one linear epitope having an amino acid sequence selected from the group consisting of: 
       
         
           
                 
               
                   i) 
                 
                   (SEQ ID NO: 65) 
                 
                   SETFTNKLKEKHTDLGKEGVTDADAKEAILKTNGTKTKGAEELGKLFE 
                 
                     
                 
                   SVEVLSKAAKEMLANSVKELTSSEEFSTKLKDNHAQLGIQGVTDENAK 
                 
                     
                 
                   KAILKANAAGKDKGVEELEKLSGSLESLSSEDFTKKLEGEHAQLGIEN 
                 
                     
                 
                   VTDENAKKAILITDAAKDKGAAELEKLFKAVENLAAKLKGEHTDLGKE 
                 
                     
                 
                   GVTDDNAKKAILKTNNDKTKGADELEKLFESVKNLSKAAKEMLTNSSE 
                 
                     
                 
                   KFAGKLKNEHASLGKKDATDDDAKKAILKTHGNTDKGAKELKDLSDSV 
                 
                     
                 
                   ESLVSDDFTKKLQSSHAQLGVAGGATTDEEAKKAILRTNAIKDKGADE 
                 
                     
                 
                   LEKLFKSVESLAKAAQDALANSVNELTSKKLKEKHTDLGKKDATDVHA 
                 
                     
                 
                   KEAILKTNGTKDKGAAELEKLFESVENLAKAAKEMLSNSNKAFTDKLK 
                 
                     
                 
                   SSHAELGIANGAATDANAKAAILKTNGTKDKGAQELEKLFESVKNLSK 
                 
                     
                 
                   AAQETLNNSSESFTKKLSDNQAELGIENATDDNAKKAILKTHNAKDKG 
                 
                     
                 
                   AEELVKLSESVAGLLKAAQAILANSVKELTSPVVAESPKKP; 
                 
                     
                 
                   ii) 
                 
                   (SEQ ID NO: 66) 
                 
                   SEKFTTKLKDSHAELGIQSVQDDNAKKAILKTHGTKDKGAKELEELFK 
                 
                     
                 
                   SLESLSKAAQAALTNSVKELTNSDKFTKKLTDSHAQLGAVGGAINDDR 
                 
                     
                 
                   AKEAILKTHGTNDKGAKELKELSESVESLAKAAQAALANSSEAFTKKL 
                 
                     
                 
                   KDSNAQLGMQNGAATDAHAKAAILKTDATKDKGATELGELFKSVESLS 
                 
                     
                 
                   KAAQEASVAFTSKLKSSNAQLGVANGNATDDDAKKAILKTNTPNDKGA 
                 
                     
                 
                   KELKELFESVESLAKAAQAALVNSVQELTNSEAFTNRLKGSHAQLGVA 
                 
                     
                 
                   AATDDHAKEAILKSNPTKDKGAKELKDLSESVESLAKAAQEALANSVK 
                 
                     
                 
                   ELTNSEAFTKKLKDNNAQLGIQNVQDVEAKKAILKTNGDISKSEAFTN 
                 
                     
                 
                   KLKEKHAELGVNGGDTTDDNAKAAIFKTHPTKDKGVEDLEKLSESVKS 
                 
                     
                 
                   LLKAAQAALSNSAAFTKKLQDGHVDLGKTDVTDDNAKEAILKTNPTKT 
                 
                     
                 
                   KGATELEELFKSVEGLVKAAKEA; 
                 
                     
                 
                   iii) 
                 
                   (SEQ ID NO: 67) 
                 
                   SEEFTNKLKSGHADLGKQDATDDHAKAAILKTHATTDKGAKEFKDLFE 
                 
                     
                 
                   SVEGLLKAAQVALTNSVKELTSKLKGGHAELGLAAATDENAKKAILKT 
                 
                     
                 
                   NGTKDKGAEELEKLFKSVESLAKAAKESLTNSVKELTNTKLRDSHAEL 
                 
                     
                 
                   GIQNVQDDNAKRAILKTHGNKDKGAKELKELSESLEKLSKAAQAALAN 
                 
                     
                 
                   SVQELTSSEAFTNKLKEKTQELAVAAGAATDIDAKKAILKTNRDKDLG 
                 
                     
                 
                   ADERGKLFKSVESLSKAAQEASANSVKELTSSEAFTDKLKNEHASLGK 
                 
                     
                 
                   KDATDDDAKKAILKTNVDKTKGADELIKLSGSLESLSKAAQAILANSE 
                 
                     
                 
                   AFTKKLQDSNADLGKHNATDADSKEAILKTNGTKTKGAKELEELFKSV 
                 
                     
                 
                   ESLSKAAKEALSNSVKELTSSQDFINKLKGGHAELGLVAATDANAKAA 
                 
                     
                 
                   ILKTNGDKTKGADEFEKLFKSVEGLLKAAQEALTNSVKELTSSEAFTK 
                 
                     
                 
                   KLQDSNADLGKHDATDADAKKAILKTDATKDK; 
                 
                     
                 
                   iv) 
                 
                   (SEQ ID NO: 68) 
                 
                   SETFTNKLKEKHTDLGKEGVTKGAEELGKLFESVEVLSKAAKEMLANS 
                 
                     
                 
                   VKELTSSEEFSTKLKDNHAQLGIQGVTKGVEELEKLSGSLESLSSEDF 
                 
                     
                 
                   TKKLEGEHAQLGIENVTAAELEKLFKAVENLAKAAKEMAKLKGEHTDL 
                 
                     
                 
                   GKEGVTKGADELEKLFESVKNLSKAAKEMLTNSKESEKFAGKLKNEHA 
                 
                     
                 
                   SLGKKDATKGAKELKDLSDSVESLVKASDDFTKKLQSSHAQLGVAGGA 
                 
                     
                 
                   TTADELEKLFKSVESLAKAAQDALANSVNELTSKKLKEKHTDLGKKDA 
                 
                     
                 
                   TAAELEKLFESVENLAKAAKEMLSNSNKAFTDKLKSSHAELGIANGAA 
                 
                     
                 
                   TKGAQELEKLFESVKNLSKAAQETLNNSVKESESETKKLSDNQAELGI 
                 
                     
                 
                   ENATKGAEELVKLSESVAGLLKAAQAILANSVKELTSPVVAESPKKP; 
                 
                     
                 
                   v) 
                 
                   (SEQ ID NO: 69) 
                 
                   SEKFTTKLKDSHAELGIQSVQDKGAKELEELFKSLESLSKAAQAALTN 
                 
                     
                 
                   SVKELTNSDKFTKKLTDSHAQLGAVGGAINDKGAKELKELSESVESLA 
                 
                     
                 
                   KAAQAALANSSEAFTKKLKDSNAQLGMQNGAATDKGATELGELFKSVE 
                 
                     
                 
                   SLSKAAQEASVAFTSKLKSSNAQLGVANGNATDKGAKELKELFESVES 
                 
                     
                 
                   LAKAAQAALVNSVQELTNSEAFTNRLKGSHAQLGVAAATDKGAKELKD 
                 
                     
                 
                   LSESVESLAKAAQEALANSVKELTNSEAFTKKLKDNNAQLGIQNVQSE 
                 
                     
                 
                   AFTNKLKEKHAELGVNGGDTTDKGVEDLEKLSESVKSLLKAAQAALSN 
                 
                     
                 
                   SAAFTKKLQDGHVDLGKTDVTTKGATELEELFKSVEGLVKAAKEA; 
                 
                     
                 
                   vi) 
                 
                   (SEQ ID NO: 70) 
                 
                   SEEFTNKLKSGHADLGKQDATKGAKEEKDLFESVEGLLKAAQVALTNS 
                 
                     
                 
                   VKELTSKLKGGHAELGLAAATKGAEELEKLFKSVESLAKAAKESLTNS 
                 
                     
                 
                   VKELTNTKLRDSHAELGIQNVQKGAKELKELSESLEKLSKAAQAALAN 
                 
                     
                 
                   SVQELTSSEAFTNKLKEKTQELAVAAGAATLGADERGKLFKSVESLSK 
                 
                     
                 
                   AAQEASANSVKELTSSEAFTDKLKNEHASLGKKDATKGADELIKLSGS 
                 
                     
                 
                   LESLSKAAQAILANSEAFTKKLQDSNADLGKHNATKGAKELEELFKSV 
                 
                     
                 
                   ESLSKAAKEALSNSVKELTSSQDFINKLKGGHAELGLVAATKGADEFE 
                 
                     
                 
                   KLFKSVEGLLKAAQEALTNSVKELTSSEAFTKKLQDSNADLGKHDAT; 
                 
                     
                 
                   vii) 
                 
                   (SEQ ID NO: 71) 
                 
                   SETFTNKLKEKHTDLGKEGVTKGAEELGKLFESVEVLSKAAKEMLANS 
                 
                     
                 
                   VKELTSKGVEELEKLSGSLESLSNKAFTDKLKSSHAELGIANGAATKK 
                 
                     
                 
                   LKEKHTDLGKKDATKGADELEKLFESVKNLSKAAKEMLTNSKEIAAEL 
                 
                     
                 
                   EKLFKAVENLAKAAKEMAKLKGEHTDLGKEGVTSEEFSTKLKDNHAQL 
                 
                     
                 
                   GIQGVTKGAKELKDLSDSVESLVKAAAELEKLFESVENLAKAAKEMLS 
                 
                     
                 
                   NSSEKFAGKLKNEHASLGKKDATSEDFTKKLEGEHAQLGIENVTKGAQ 
                 
                     
                 
                   ELEKLFESVKNLSKAAQETLNNSVKEADELEKLFKSVESLAKAAQDAL 
                 
                     
                 
                   ANSVNELTSSESFTKKLSDNQAELGIENATSDDFTKKLQSSHAQLGVA 
                 
                     
                 
                   GGATTKGAEELVKLSESVAGLLKAAQAILANSVKELTSPVVAESPKKP; 
                 
                     
                 
                   viii) 
                 
                   (SEQ ID NO: 72) 
                 
                   SEAFTKKLKDSNAQLGMQNGAATDKGAKELEELFKSLESLSKAAQAAL 
                 
                     
                 
                   TNSVKELTNKDKGAKELKELFESVESLAKAAQAALVNSVQELTNSEKF 
                 
                     
                 
                   TTKLKDSHAELGIQSVQSDKFTKKLTDSHAQLGAVGGAINDKGAKELK 
                 
                     
                 
                   ELSESVESLAKAAQAALANSDKGAKELKDLSESVESLAKAAQEALANS 
                 
                     
                 
                   VKELTNSVAFTSKLKSSNAQLGVANGNATSEAFTKKLKDNNAQLGIQN 
                 
                     
                 
                   VQTKGATELEELFKSVEGLVKAAKEADKGVEDLEKLSESVKSLLKAAQ 
                 
                     
                 
                   AALSNSAAFTKKLQDGHVDLGKTDVTSEAFTNRLKGSHAQLGVAAATD 
                 
                     
                 
                   KGATELGELFKSVESLSKAAQEASEAFTNKLKEKHAELGVNGGDTT; 
                 
                   and 
                 
                   ix) 
                 
                   (SEQ ID NO: 73) 
                 
                   SEEFTNKLKSGHADLGKQDATKGAKEFKDLFESVEGLLKAAQVALTNS 
                 
                     
                 
                   VKELTSKEKGAEELEKLFKSVESLAKAAKESLTNSVKELTNSEAFTDK 
                 
                     
                 
                   LKNEHASLGKKDATTKLRDSHAELGIQNVQLGADERGKLFKSVESLSK 
                 
                     
                 
                   AAQEASANSVKELTSKEKGAKELEELFKSVESLSKAAKEALSNSVKEL 
                 
                     
                 
                   TSSEAFTKKLQDSNADLGKHNATSEAFTKKLQDSNADLGKHDATKGAD 
                 
                     
                 
                   EFEKLEKSVEGLLKAAQEALTNSVKELTSELKELSESLEKLSKAAQAA 
                 
                     
                 
                   LANSVQELTSSEAFTNKLKEKTQELAVAAGAATKLKGGHAELGLAAAT 
                 
                     
                 
                   KGADELIKLSGSLESLSKAAQAILANSQDFINKLKGGHAELGLVAAT. 
                 
             
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
                
               
            
           
         
       
     
     
         19 . The isolated recombinant or synthetic peptide or polypeptide of  claim 18 , further comprising one or more epitopes of  Borrelia  outer surface protein A (OspA).
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