Methods for detection or measurement of viruses

Abstract

A method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) an anionic surfactant and (2) an amphoteric surfactant, nonionic surfactant or protein denaturant; a virus assay method using said treating method; a method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) a chaotropic ion and (2) an acidifying agent; a virus assay method using said treating method; a virus assay method, characterized in that a virus antigen and a virus antibody are measured based on their binding to their probe in the presence of a surfactant with an alkyl group of 10 or more carbon atoms and a secondary, tertiary or quaternary amine, or a nonionic surfactant, or of both of them; and a monoclonal antibody and a hybridoma producing the same for carrying out said method.

1 . A method for a treating virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) an anionic surfactant and (2) an amphoteric surfactant, nonionic surfactant or protein denaturant. 
     
     
         2 . A method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) an anionic surfactant, (2) an amphoteric surfactant and (3) a nonionic surfactant or protein denaturant. 
     
     
         3 . A method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) an anionic surfactant, (2) an amphoteric surfactant, (3) nonionic surfactant and (4) a protein denaturant. 
     
     
         4 . A method according to any one of  claims 1  to  4 , wherein said treatment solution further contains urea, an imidazole ring-containing compound or an indole ring-containing compound. 
     
     
         5 . A method according to  claim 4 , wherein said imidazole ring-containing compound is imidazole, histidine, imidazoleacrylic acid, imidazolecarboxyaldehyde, imidazolecarboxamide, imidazoledione, imidazoledithiocarboxylic acid, imidazoledicarboxylic acid, imidazolemethanol, imidazolidinethione, imidazolidone, histamine or imidazopyridine. 
     
     
         6 . A method according to  claim 4 , wherein said indole ring-containing compound is tryptophan, indoleacrylic acid, indole, indoleacetic acid, indoleacetic hydrazide, methyl indoleacetate, indolebutyric acid, indoleacetonitrile, indolecarbinol, indolecarboxyaldehyde, indolecarboxylic acid, indoleethanol, indolelactic acid, indolemethanol, indolepropionic acid, indolepyruvic acid, indolyl methyl ketone, indomycin, indoleacetone, indomethacin, indoprofen or indolamine. 
     
     
         7 . A method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) a chaotropic ion and (2) an acidifying agent. 
     
     
         8 . A method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) a chaotropic ion, (2) an acidifying agent and (3) a nonionic surfactant. 
     
     
         9 . A method according to any one of  claims 1  to  8 , wherein said virus is a virus which forms virus particles having a structure comprising a structural protein encapsulating genomic RNA or DNA and a membrane protein or lipid membrane surrounding it. 
     
     
         10 . A method according to  claim 9 , wherein said virus is hepatitis C virus (HCV), hepatitis D virus, hepatitis E virus, hepatitis G virus, hand-foot-and-mouth disease virus, a flavivirus (yellow fever virus, West Nile virus, Japanese encephalitis virus, dengue virus), a togavirus (alpha-virus, rubivirus, arterivirus, rubella virus), a pestivirus (hog cholera virus, bovine diarrhea virus), a paramyxovirus (parainfluenza virus 1, 2, 3, 4, canine distemper virus, Newcastle disease virus, RS virus, rinderpest virus, simian parainfluenza virus, measles virus, mumps virus), an orthomyxovirus (human influenza virus, avian influenza virus, equine influenza virus, swine influenza virus), a rhabdovirus (rabies virus, vesicular stomatitis virus), a picornavirus (poliovirus, Coxsackie virus, echovirus, bovine enterovirus, porcine enterovirus, simian enterovirus, mouse encephalitis virus, human rhinovirus, bovine rhinovirus, equine rhinovirus, foot and mouth disease virus, hepatitis A virus), a coronavirus (human coronavirus, avian infectious bronchitis virus, mouse hepatitis virus, porcine transmissible gastroenteritis virus), an arenavirus (lymphocytic choriomeningitis virus, lassa virus, Korean hemorrhagic fever virus), a retrovirus (HTLV: human adult leukemia virus, HIV: AIDS virus, feline leukemia sarcoma virus, bovine leukemia virus, Rous sarcoma virus), a reovirus (rotavirus), a calcivirus (Norwalk virus), a bunyavirus (renal syndrome hemorrhagic fever virus), a phyllovirus (Ebola virus, Marburg virus), hepatitis B virus (HBV), a pox virus (vaccinia virus, alastrim virus, cowpox virus, smallpox virus), a parvovirus (human parvovirus, porcine parvovirus, bovine parvovirus, canine parvovirus, feline leucopenia virus, Aleutian mink disease virus), a papovavirus (papilloma virus, polyoma virus), adenovirus, a herpes virus (herpes simplex virus, cytomegalovirus, chickenpox herpes zoster virus, EB virus, equine herpes virus, feline herpes virus, Marek's disease virus) or African swine cholera virus. 
     
     
         11 . A method according to any one of  claims 1  to  10 , wherein said virus is hepatitis C virus (HCV) or hepatitis B virus (HBV). 
     
     
         12 . A virus assay method, characterized by using a sample treating method according to any one of  claims 1  to  10  and reacting it with a probe which specifically recognizes a virus antigen, for detection or quantitation of the presence of the virus antigen. 
     
     
         13 . A hybridoma cell line selected from the group consisting of HC11-11 (FERM BP-6005), HC11-14 (FERM BP-6006), HC11-10 (FERM BP-6004), HC11-3 (FERM BP-6002) and HC11-7 (FERM BP-6003). 
     
     
         14 . A monoclonal antibody produced by a hybridoma selected from the group consisting of HC11-11 (FERM BP-6005), HC11-14 (FERM BP-6006), HC11-10 (FERM BP-6004), HC11-3 (FERM BP-6002) and HC11-7 (FERM BP-6003). 
     
     
         15 . A kit, assay kit or diagnostic reagent for determining the presence or absence of a virus in a sample, which is for use in an immunoassay method according to  claim 12  and comprises an anionic surfactant. 
     
     
         16 . A kit, assay kit or diagnostic reagent for determining the presence or absence of a virus in a sample, which is for use in an immunoassay method according to  claim 12  and comprises a monoclonal antibody according to  claim 14 . 
     
     
         17 . A kit, assay kit or diagnostic reagent for determining the presence or absence of a virus in a sample, which is for use in an immunoassay method according to  claim 12  and comprises a chaotropic agent. 
     
     
         18 . A kit, assay kit or diagnostic reagent for determining the presence or absence of HCV in a sample, which is for use in an immunoassay method according to  claim 12  and comprises a monoclonal antibody produced by hybridoma HC11-14 (FERM BP-6006), HC11-10 (FERM BP-6004) or HC11-11 (FERM BP-6005). 
     
     
         19 . A diagnostic kit according to any one of  claims 15  to  17  which further includes urea, an imidazole ring-containing compound or an indole ring-containing compound. 
     
     
         20 . A diagnostic kit according to  claim 19 , wherein said imidazole ring-containing compound is imidazole, histidine, imidazoleacrylic acid, imidazolecarboxyaldehyde, imidazolecarboxamide, imidazoledione, imidazoledithiocarboxylic acid, imidazoledicarboxylic acid, imidazolemethanol, imidazolidinethione, imidazolidone, histamine or imidazopyridine. 
     
     
         21 . A diagnostic kit according to  claim 19 , wherein said indole ring-containing compound is tryptophan, indoleacrylic acid, indole, indoleacetic acid, indoleacetic hydrazide, methyl indoleacetate, indolebutyric acid, indoleacetonitrile, indolecarbinol, indolecarboxyaldehyde, indolecarboxylic acid, indoleethanol, indolelactic acid, indolemethanol, indolepropionic acid, indolepyruvic acid, indolyl methyl ketone, indomycin, indoleacetone, indomethacin, indoprofen or indolamine. 
     
     
         22 . A virus assay method characterized by measurement of a virus antigen based on its binding with a probe in the presence of a surfactant with an alkyl group of 10 or more carbon atoms and a secondary, tertiary or quaternary amine, or a nonionic surfactant with a hydrophilic/lipophilic balance (HLB) of 12-14. 
     
     
         23 . A method according to  claim 22 , wherein said surfactant having an alkyl group and a secondary, tertiary or quaternary amine is a surfactant with an alkyl group of 10-20 carbon atoms and a tertiary or quaternary amine. 
     
     
         24 . A method according to  claim 22  or  23 , wherein said tertiary or quaternary amine surfactant is dodecyl-N-sarcosinic acid, a cetyl or dodecyltrimethylammonium salt, 3-(dodecyldimethylammonio)-1-propanesulfonic acid, a dodecylpyrimidium salt or decanoyl-N-methylglucamide (MEGA-10). 
     
     
         25 . A method according to either of  claim 23  or  24 , wherein said nonionic surfactant is polyoxyethylene isooctyl phenyl ether or polyoxyethylene nonyl phenyl ether. 
     
     
         26 . A method according to any one of  claims 22  to  25 , wherein said virus antigen probe is an antibody for the virus antigen. 
     
     
         27 . A method according to any one of  claims 22  to  26 , wherein said virus is a virus which forms virus particles having a structure comprising a structural protein encapsulating genomic RNA or DNA and a membrane protein or lipid membrane surrounding it. 
     
     
         28 . A method according to  claim 27 , wherein said virus is hepatitis C virus (HCV), hepatitis D virus, hepatitis E virus, hepatitis G virus, hand-foot-and-mouth disease virus, a flavivirus (yellow fever virus, West Nile virus, Japanese encephalitis virus, dengue virus), a togavirus (alpha-virus, rubivirus, arterivirus, rubella virus), a pestivirus (hog cholera virus, bovine diarrhea virus), a paramyxovirus (parainfluenza virus 1, 2, 3, 4, canine distemper virus, Newcastle disease virus, RS virus, rinderpest virus, simian parainfluenza virus, measles virus, mumps virus), an orthomyxovirus (human influenza virus, avian influenza virus, equine influenza virus, swine influenza virus), a rhabdovirus (rabies virus, vesicular stomatitis virus), a picornavirus (poliovirus, Coxsackie virus, echovirus, bovine enterovirus, porcine enterovirus, simian enterovirus, mouse encephalitis virus, human rhinovirus, bovine rhinovirus, equine rhinovirus, foot and mouth disease virus, hepatitis A virus), a coronavirus (human coronavirus, avian infectious bronchitis virus, mouse hepatitis virus, porcine transmissible gastroenteritis virus), an arenavirus (lymphocytic choriomeningitis virus, lassa virus, Korean hemorrhagic fever virus), a retrovirus (HTLV: human adult leukemia virus, HIV: AIDS virus, feline leukemia sarcoma virus, bovine leukemia virus, Rous sarcoma virus), a reovirus (rotavirus), a calcivirus (Norwalk virus), a bunyavirus (renal syndrome hemorrhagic fever virus), a phyllovirus (Ebola virus, Marburg virus), hepatitis B virus (HBV), a pox virus (vaccinia virus, alastrim virus, cowpox virus, smallpox virus), a parvovirus (human parvovirus, porcine parvovirus, bovine parvovirus, canine parvovirus, feline leucopenia virus, Aleutian mink disease virus), a papovavirus (papilloma virus, polyoma virus), adenovirus, a herpes virus (herpes simplex virus, cytomegalovirus, chickenpox herpes zoster virus, EB virus, equine herpes virus, feline herpes virus, Marek's disease virus) or African swine cholera virus. 
     
     
         29 . A method according to any one of  claims 22  to  28 , wherein said virus is hepatitis C virus (HCV) or hepatitis B virus (HBV).