Microstamping Activated Polymer Surfaces

Abstract

Methods of attaching a ligand to a surface are described that include contacting a surface with a substrate containing an amphiphilic comb polymer. The substrate is configured to provide a pattern of the amphiphilic comb polymer on a selected region of the surface. The substrate can be separated from the surface leaving the amphiphilic comb polymer on the selected region of the surface, thus providing a selected region of the surface having amphiphilic comb polymer on it. A ligand can then be deposited on the surface such that the selected region of the surface having the amphiphilic comb polymer is substantially free of the ligand.

1 - 3 . (canceled) 
     
     
         4 . The device of  claim 36 , wherein the substrate is a stamp, and wherein the at least one ligand is attached to a surface of the stamp. 
     
     
         5 . The device of  claim 36 , wherein the substrate comprises at least one well and wherein an aqueous solution is present in the at least one well, the aqueous solution comprising the at least one ligand. 
     
     
         6 . The device of  claim 36 , wherein the substrate is configured to provide a pattern of the at least one ligand on the surface. 
     
     
         7 . (canceled) 
     
     
         8 . The device of  claim 36 , wherein the amphiphilic comb polymer comprises a backbone formed of a hydrophobic water-insoluble polymer and at least one side chain formed of a hydrophilic polymer. 
     
     
         9 - 15 . (canceled) 
     
     
         16 . The device of  claim 36 , wherein the ligand is selected from the group consisting of small biological molecules, proteins, peptides, nucleic acids, lipids, saccharides, oligosaccharides, carbohydrates, lipopolysaccharide, lipoprotein, peptide nucleic acids (PNA), ribozymes, DNA or PNA aptamer. 
     
     
         17 - 29 . (canceled) 
     
     
         30 . The device of  claim 36 , wherein a plurality of ligands are adsorbed on the stamp in a pattern, and wherein the pattern of ligands is covalently bound to the surface after the separating step. 
     
     
         31 . (canceled) 
     
     
         32 . The device of  claim 36 , wherein the second reactive moiety of the ligand is linked to the ligand by a spacer. 
     
     
         33 - 35 . (canceled) 
     
     
         36 . A device comprising at least one surface having a ligand bound thereto according to a method of attaching a ligand to a surface, said method comprising:
 contacting a surface having an amphiphilic comb polymer present thereon, said amphiphilic comb polymer having a first reactive moiety attached thereto with a substrate having at least one ligand thereon, said ligand comprising a second reactive moiety, wherein the second reactive moiety of the bond and the first reactive moiety of the amphiphilic comb polymer form a covalent bond; and then   separating the substrate from the surface, thereby leaving the ligand covalently bound to the amphiphilic comb polymer.   
     
     
         37 - 39 . (canceled) 
     
     
         40 . The device of  claim 68 , wherein the substrate comprises a stamp, and wherein the amphiphilic comb polymer is attached to a surface of the stamp. 
     
     
         41 . The device of  claim 68 , wherein the substrate comprises at least one well and wherein an aqueous solution is present in the at least one well, the aqueous solution comprising the amphiphilic comb polymer. 
     
     
         42 . The device of  claim 68 , wherein the ligand is deposited by adsorption from a solution. 
     
     
         43 . The device of  claim 68 , wherein the ligand is deposited by chemical conjugation from a solution. 
     
     
         44 . The device of  claim 68 , wherein the amphiphilic comb polymer has a first reactive moiety attached thereto and the ligand has a second reactive moiety attached thereto, wherein the second reactive moiety of the ligand and the first reactive moiety of the amphiphilic comb polymer form a covalent bond. 
     
     
         45 . The device of  claim 68 , wherein the amphiphilic comb polymer comprises a backbone formed of a hydrophobic water-insoluble polymer and at least one side chain formed of a hydrophilic polymer. 
     
     
         46 - 50 . (canceled) 
     
     
         51 . The device of  claim 68 , wherein the ligand is selected from the group consisting of small biological molecules, proteins, peptides, nucleic acids, lipids, saccharides, oligosaccharides, carbohydrates, lipopolysaccharide, lipoprotein, peptide nucleic acids (PNA), ribozymes, DNA or PNA aptamer. 
     
     
         52 - 67 . (canceled) 
     
     
         68 . A device comprising at least one surface having a ligand bound thereto according to a method of attaching a ligand to a surface, said method comprising:
 contacting a surface with a substrate containing an amphiphilic comb polymer wherein the substrate is configured to provide a pattern of the amphiphilic comb polymer on a selected region of the surface;   separating the substrate from the surface, thereby leaving the amphiphilic comb polymer on the selected region of the surface to provide a selected region of the surface having amphiphilic comb polymer thereon; and then   depositing a ligand on the surface such that the selected region of the surface having the amphiphilic comb polymer thereon is substantially free of the ligand.   
     
     
         69 - 71 . (canceled) 
     
     
         72 . The device of  claim 105 , wherein the amphiphilic comb polymer is physically bound to the ligand. 
     
     
         73 . The device of  claim 105 , wherein the ligand comprises a first reactive moiety attached thereto and the amphiphilic comb polymer comprises a second reactive moiety; and wherein during the contacting step, the second reactive moiety of the amphiphilic comb polymer and the first reactive moiety of the ligand form a covalent bond. 
     
     
         74 . (canceled) 
     
     
         75 . The device of  claim 105 , wherein the ligand is deposited by adsorption from a solution. 
     
     
         76 . The device of  claim 105 , wherein the substrate is a stamp, and wherein the amphiphilic comb polymer is attached to a surface of the stamp. 
     
     
         77 . The device of  claim 105 , wherein the substrate comprises at least one well and wherein an aqueous solution is present in the at least one well, the aqueous solution comprising the amphiphilic comb polymer. 
     
     
         78 . The device of  claim 105 , wherein the substrate is configured to provide a pattern of the amphiphilic comb polymer on the surface. 
     
     
         79 . (canceled) 
     
     
         80 . The device of  claim 105 , wherein the amphiphilic comb polymer comprises a backbone formed of a hydrophobic water-insoluble polymer and at least one side chain formed of a hydrophilic polymer. 
     
     
         81 - 87 . (canceled) 
     
     
         88 . The device of  claim 105 , wherein the ligand is selected from the group consisting of small biological molecules, proteins, peptides, nucleic acids, lipids, saccharides, oligosaccharides, carbohydrates, lipopolysaccharide, lipoprotein, peptide nucleic acids (PNA), ribozymes, DNA or PNA aptamer. 
     
     
         89 - 104 . (canceled) 
     
     
         105 . A device comprising at least one surface having a ligand bound thereto according to a method of attaching a ligand to a surface, said method comprising:
 depositing at least one ligand on a surface;   contacting the surface having the ligand present thereon with a substrate having an amphiphilic comb polymer thereon; and then   separating the substrate from the surface, thereby leaving the amphiphilic comb polymer bound to the ligand.