Devices and methods for enrichment and alteration of circulating tumor cells and other particles

Abstract

The invention features devices and methods for detecting, enriching, and analyzing circulating tumor cells and other particles. The invention further features methods of diagnosing a condition, e.g., cancer, in a subject by analyzing a cellular sample from the subject.

1 .- 8 . (canceled) 
     
     
         9 . A microfluidic device comprising: a microfluidic channel, said channel comprising an inlet, an outlet and a micro-corrugated surface, wherein at least one surface of said channel comprises at least one capture moiety that selectively captures a first cell from a cellular sample. 
     
     
         10 . The device of  claim 9 , wherein the at least one capture moiety is an antibody. 
     
     
         11 . The device of  claim 10 , wherein the antibody is an anti-EpCAM antibody. 
     
     
         12 . The device of  claim 9 , wherein the at least one surface comprising a capture moiety is the micro-corrugated surface. 
     
     
         13 . The device of  claim 9 , wherein the micro-corrugated surface is the top surface of the channel. 
     
     
         14 . The device of  claim 9 , wherein the device is configured to retain at least 50% of the first cells present in the cellular sample. 
     
     
         15 . The device of  claim 9 , wherein the device is configured to retain at least 80% of the first cells present in the cellular sample. 
     
     
         16 . The device of  claim 9 , wherein the device is configured for a flow rate of up to 6 mL/hour. 
     
     
         17 . The device of  claim 15 , wherein the device is configured for a flow rate of up to 6 mL/hour. 
     
     
         18 . The device of  claim 9 , wherein at least part of the device is transparent. 
     
     
         19 . The device of  claim 9 , further comprising an imaging device. 
     
     
         20 . The device of  claim 19 , wherein said imaging device is a microscope or spectrometer. 
     
     
         21 . A microfluidic device for the capture of a target cell, comprising: a microfluidic channel comprising an inlet, an outlet, a first array comprising at least one obstacle and a second array comprising at least one obstacle, wherein the obstacles of the first array and the second array are micro-corrugations and wherein at least one surface of said channel comprises at least one capture moiety that selectively captures a first cell from a cellular sample. 
     
     
         22 . The device of  claim 21 , wherein the at least one obstacle in said first array is offset by less than half of the period of the at least one obstacle in the second array. 
     
     
         23 . The device of  claim 21 , wherein the at least one obstacle in the first array is not aligned with the at least one obstacle in the second array. 
     
     
         24 . The device of  claim 21 , wherein the at least one capture moiety is an antibody. 
     
     
         25 . The device of  claim 24 , wherein the antibody is an anti-EpCAM antibody. 
     
     
         26 . The device of  claim 21 , wherein the at least one surface comprising a capture moiety is the micro-corrugated surface. 
     
     
         27 . The device of  claim 21 , wherein the micro-corrugated surface is the top surface of the channel. 
     
     
         28 . The device of  claim 21 , wherein the device is configured to retain at least 50% of the first cells present in the cellular sample. 
     
     
         29 . The device of  claim 21 , wherein the device is configured to retain at least 80% of the first cells present in the cellular sample. 
     
     
         30 . The device of  claim 21 , wherein the device is configured for a flow rate of up to 6 mL/hour. 
     
     
         31 . The device of  claim 29 , wherein the device is configured for a flow rate of up to 6 mL/hour. 
     
     
         32 . The device of  claim 21 , wherein at least part of the device is transparent. 
     
     
         33 . A method for capturing particles in a fluid flowed through a microfluidic channel, comprising:
 flowing the fluid comprising the particles through the microfluidic channel, said channel comprising a micro-corrugated surface;   contacting one or more or the particles on a capture moiety disposed on at least one surface of the microfluidic channel; and   capturing one or more of the particles contacting the capture moiety.   
     
     
         34 . The method of  claim 33 , wherein the at least one surface comprising a capture moiety is the micro-corrugated surface. 
     
     
         35 . The method of  claim 33 , wherein the particles are cancer cells. 
     
     
         36 . The method of  claim 33 , wherein the capture moiety is an antibody.