US2011300540A1PendingUtilityA1

Methods and probe combinations for detecting melanoma

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Assignee: BASTIAN BORISPriority: Sep 2, 2005Filed: Jun 2, 2011Published: Dec 8, 2011
Est. expirySep 2, 2025(expired)· nominal 20-yr term from priority
Inventors:Boris BastianLarry E. MorrisonSusan Jewell
C12Q 1/6841C12Q 2600/112C12Q 2537/143C12Q 1/6886C12Q 2600/158
56
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Claims

Abstract

The present invention is based on the discovery of methods and combinations of probes to chromosomal regions that are gained or lost or imbalanced in melanoma that provide highly specific and sensitive assays for the detection of melanoma cells.

Claims

exact text as granted — not AI-modified
1 - 17 . (canceled) 
     
     
         18 . A combination of between two and ten probes comprising a probe that targets chromosome region 6p and a probe that targets chromosome region 6q, wherein the combination of probes has a difference from ideal (DFI) value of less than about 0.29 for melanoma. 
     
     
         19 . The combination of probes of  claim 18 , wherein the combination of probes has a DFI value of less than about 0.20. 
     
     
         20 . The combination of probes of  claim 18 , wherein one of the probes in the combination of between two and ten probes is a probe that targets chromosome subregion 6p25. 
     
     
         21 . The combination of probes of  claim 18 , wherein the combination is a combination of at least three probes, wherein an additional probe is selected from the group consisting of a chromosome 6 enumerator probe a chromosome 10 enumerator probe, a probe that targets chromosome region 7q, a probe that targets chromosome region 11q, a probe that targets chromosome region 17q, a probe that targets chromosome region 1q, and a probe that targets chromosome region 20q. 
     
     
         22 . The combination of probes of  claim 21 , wherein the three probes are selected from the group of probes consisting of: a probe that targets chromosome subregion 6p25, a probe that targets chromosome subregion 6q23 and a probe that targets chromosome subregion 11q13; a probe that targets chromosome subregions 6p25, a probe that targets chromosome subregion 6q23 and a chromosome 6 enumerator probe; and a probe that targets chromosome subregion 6p25, a probe that targets chromosome subregion 20q13 and a probe that targets chromosome subregion 6q23. 
     
     
         23 . The combination of probes of  claim 18 , wherein the combination is a combination of at least four probes, wherein two additional probes are selected from the group consisting of a chromosome 6 enumerator probe, a chromosome 10 enumerator probe, a probe that targets chromosome region 7q, a probe that targets chromosome region 11q, a probe that targets chromosome region 17q, a probe that targets chromosome region 1q, and a probe that targets chromosome region 20q. 
     
     
         24 . The combination of probes of  claim 23 , wherein the four probes are selected from the group consisting of: a probe that targets chromosome subregion 6p25; a probe that targets chromosome subregion 6q23, a chromosome 6 enumerator probe, and a chromosome 10 enumerator probe; a probe that targets chromosome subregion 6p25; a probe that targets chromosome subregion 6q23, a probe that targets chromosome subregion 11q13, and a chromosome 6 enumerator probe; a probe that targets chromosome subregion 6p25; a probe that targets chromosome subregion 6q23, a probe that targets chromosome subregion 11q13, and a chromosome 10 enumerator probe; a probe that targets chromosome subregion 6p25; a probe that targets chromosome subregion 6q23, a probe that targets chromosome subregion 1q31, and a chromosome 10 enumerator probe; a probe that targets chromosome subregion 6p25, a probe that targets chromosome subregion 17q25, a probe that targets chromosome subregion 6q23, and a chromosome 6 enumerator probe; a probe that targets chromosome subregion 6p25, a probe that targets chromosome subregion 20q13, a probe that targets chromosome subregion 6q23, and a chromosome 6 enumerator probe; a probe that targets chromosome subregion 6p25, a probe that targets chromosome subregion 7q34, a probe that targets chromosome subregion 6q23, and a chromosome 6 enumerator probe; a probe that targets chromosome subregion 6p25, a probe that targets chromosome subregion 7q34, a probe that targets chromosome subregion 6q23, and a probe that targets chromosome subregion 17q25; a probe that targets chromosome subregion 6p25, a probe that targets chromosome subregion 20q13, a probe that targets chromosome subregion 6q23, and a probe that targets chromosome subregion 17q25; and a probe that targets chromosome subregion 6p25, a probe that targets chromosome subregion 6q23, a probe that targets chromosome subregion 17q25, and a chromosome 10 enumerator probe. 
     
     
         25 . The combination of probes of  claim 20 , wherein a second probe in the combination of between two and ten probes targets chromosome region 6q23. 
     
     
         26 . The combination of probes of  claim 21 , wherein the combination of three probes has at least two probes selected from the group of two probes consisting of: a probe that targets chromosome subregion 6q23 and a chromosome 6 enumerator probe; a probe that targets chromosome subregion 20q13 and a probe that targets chromosome subregion 6q23. 
     
     
         27 . The combination of probes of  claim 23 , wherein the combination of four probes has at least three probes selected from the group of three probes consisting of: a probe that targets chromosome subregion 6q23, a chromosome 6 enumerator probe, and a chromosome 10 enumerator probe; a probe that targets chromosome subregion 6q23, a probe that targets chromosome subregion 11q13, and a chromosome 6 enumerator probe; a probe that targets chromosome subregion 6q23, a probe that targets chromosome subregion 11q13, and a chromosome 10 enumerator probe; a probe that targets chromosome subregion 6q23, a probe that targets chromosome subregion 1q31, and a chromosome 10 enumerator probe; a probe that targets chromosome subregion 17q25, a probe that targets chromosome subregion 6q23, and a chromosome 6 enumerator probe; a probe that targets chromosome subregion 20q13, a probe that targets chromosome subregion 6q23, and a chromosome 6 enumerator probe; a probe that targets chromosome subregion 7q34, a probe that targets chromosome subregion 6q23, and a chromosome 6 enumerator probe; a probe that targets chromosome subregion 7q34, a probe that targets chromosome subregion 6q23, and a probe that targets chromosome subregion 17q25; a probe that targets chromosome subregion 20q13, a probe that targets chromosome subregion 6q23, and a probe that targets chromosome subregion 17q25; a probe that targets chromosome subregion 6q23, and a probe that targets chromosome subregion 17q25, and a chromosome 10 enumerator probe. 
     
     
         28 . A kit for diagnosing melanoma with a difference from ideal (DFI) value of less than about 0.29, the kit comprising the combination of probes of  claim 18 . 
     
     
         29 . The kit of  claim 28 , wherein the kit comprises the combination of probes of  claim 19 . 
     
     
         30 . The kit of  claim 28 , wherein the kit comprises the combination of probes of  claim 20 . 
     
     
         31 . The kit of  claim 28 , wherein the kit comprises the combination of probes of  claim 21 . 
     
     
         32 . The kit of  claim 28 , wherein the kit comprises the combination of probes of  claim 22 . 
     
     
         33 . The kit of  claim 28 , wherein the kit comprises the combination of probes of  claim 23 . 
     
     
         34 . The kit of  claim 28 , wherein kit comprises the combination of probes of  claim 24 . 
     
     
         35 . The kit of  claim 28 , wherein the kit comprises the combination of probes of  claim 25 . 
     
     
         36 . The kit of  claim 28 , wherein the kit comprises the combination of probes of  claim 26 . 
     
     
         37 . The kit of  claim 28 , wherein the kit comprises the combination of probes of  claim 27 . 
     
     
         38 . The combination of probes of  claim 18  or the kit of  claim 28 , wherein the combination of between two and ten probes comprises a probe that targets chromosome subregion 6p25, a probe that targets chromosome region 6q, and a chromosome 6 enumerator probe. 
     
     
         39 . The combination of probes of  claim 18  or the kit of  claim 28 , wherein the combination of probes comprises a probe that targets chromosome subregion 6p25, a probe that targets chromosome subregion 6q23, and a chromosome 6 enumerator probe. 
     
     
         40 . The combination of probes of  claim 18  or the kit of  claim 28 , wherein the combination of between two and ten probes comprises a probe that targets chromosome subregion 6p25, a probe that targets chromosome region 6q, a probe that targets chromosome region 11q. 
     
     
         41 . The combination of probes of  claim 18  or the kit of  claim 28 , wherein the combination of between two and ten probes comprises a probe that targets chromosome subregion 6p25, a probe that targets chromosome subregion 6q23, and a probe that targets chromosome region 11q. 
     
     
         42 . The combination of probes of  claim 18  or the kit of  claim 28 , wherein the combination of between two and ten probes comprises a probe that targets chromosome subregion 6p25, a probe that targets chromosome region 6q, and a probe that targets chromosome subregion 11q13. 
     
     
         43 . The combination of probes of  claim 18  or the kit of  claim 28 , wherein the combination of between two and ten probes comprises a probe that targets chromosome subregion 6p25, a probe that targets chromosome subregion 6q23, and a probe that targets chromosome subregion 11q13. 
     
     
         44 . The combination of probes of  claim 18  or the kit of  claim 28 , wherein the combination of between two and ten probes comprises a probe that targets chromosome subregion 6p25, a probe that targets chromosome region 6q, a chromosome 6 enumerator probe, and a probe that targets chromosome region 11q. 
     
     
         45 . The combination of probes of  claim 18  or the kit of  claim 28 , wherein the combination of between two and ten probes comprises a probe that targets chromosome subregion 6p25, a probe that targets chromosome subregion 6q23, a chromosome 6 enumerator probe, and a probe that targets chromosome region 11q. 
     
     
         46 . The combination of probes of  claim 18  or the kit of  claim 28 , wherein the combination of between two and ten probes comprises a probe that targets chromosome subregion 6p25, a probe that targets chromosome region 6q, a chromosome 6 enumerator probe, and a probe that targets chromosome subregion 11q13. 
     
     
         47 . The combination of probes of  claim 18  or the kit of  claim 28 , wherein the combination of between two and ten probes comprises a probe that targets chromosome subregion 6p25, a probe that targets chromosome subregion 6q23, a chromosome 6 enumerator probe, and a probe that targets chromosome subregion 11q13. 
     
     
         48 . The combination of probes of  claim 18  or the kit of  claim 28 , wherein the combination of between two and ten probes comprises not more than four probes. 
     
     
         49 . The combination of probes of  claim 18  or the kit of  claim 28 , wherein the combination probes has a difference from ideal (DFI) value of less than about 0.29 for distinguishing primary melanoma from benign nevi.

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