Methods for generating anti-il-23 antibodies, compositions, methods and uses

Abstract

A protein variant of IL-23p19 that has conserved residues in positions 93-102, 93-110, and/or 127-137 of SEQ ID NO:1, can be used to generate anti-IL-23p19 antibodies.

1 . A method for generating an antibody, comprising panning an antibody library with an isolated IL-23p19 variant protein, comprising an amino acid sequence having less than 50% identity to the amino acid sequence of SEQ ID NO:1 wherein said protein has the identical amino acid sequence at residues 93-102, 93-110, and/or 127-137 of SEQ ID NO:1, and isolating an antibody or antibody fragment binding to the IL-23p19 variant protein. 
     
     
         2 . The method of  claim 1 , wherein said protein has less than 60% identity to the amino acid sequence of SEQ ID NO:1. 
     
     
         3 . The method of  claim 1 , wherein said protein has less than 70% identity to the amino acid sequence of SEQ ID NO:1. 
     
     
         4 . The method of  claim 1 , wherein said protein has less than 80% identity to the amino acid sequence of SEQ ID NO:1. 
     
     
         5 . The method of  claim 1 , wherein said protein has less than 90% identity to the amino acid sequence of SEQ ID NO:1. 
     
     
         6 . The method of  claim 1 , wherein said protein has less than 95% identity to the amino acid sequence of SEQ ID NO:1. 
     
     
         7 . A method for generating an antibody, comprising contacting an animal producing antibodies with an isolated IL-23p19 variant protein, comprising an amino acid sequence having less than 50% identity to the amino acid sequence of SEQ ID NO:1 wherein said protein has the identical amino acid sequence at residues 93-102, 93-110, and/or 127-137 of SEQ ID NO:1, and isolating an antibody or antibody fragment binding to the IL-23p19 variant protein. 
     
     
         8 . The method of  claim 7 , wherein said protein has less than 60% identity to the amino acid sequence of SEQ ID NO:1. 
     
     
         9 . The method of  claim 7 , wherein said protein has less than 70% identity to the amino acid sequence of SEQ ID NO:1. 
     
     
         10 . The method of  claim 7 , wherein said protein has less than 80% identity to the amino acid sequence of SEQ ID NO:1. 
     
     
         11 . The method of  claim 7 , wherein said protein has less than 90% identity to the amino acid sequence of SEQ ID NO:1. 
     
     
         12 . The method of  claim 7 , wherein said protein has less than 95% identity to the amino acid sequence of SEQ ID NO:1. 
     
     
         13 . The method of  claim 7 , wherein the animal producing antibodies is selected from the group consisting of mice, rats, rabbits, camel, goats, cows, horses, sheep, and non-human primates. 
     
     
         14 . The method of  claim 13 , wherein the animal producing antibodies is a transgenic animal capable of producing antibodies with human sequences. 
     
     
         15 . Any invention described herein.