Water Dispersible Glyceryl Monooleate Magnetic Nanoparticle Formulation

Abstract

The present invention is an aqueous dispersible magnetic nanoparticle formulation with a high drug loading capacity used for sustained drug delivery. The formulated magnetic nanoparticles are composed of an iron oxide core coated with a long chain polymer, which provides aqueous dispersibility without the use of surfactant. A method is developed for the functionalization of magnetic nanoparticles for use in biomedical field.

1 . A water dispersible glyceryl monooleate (GMO) magnetic nanoparticle (MNP) formulation comprising an iron oxide particle core coated with a long chain polymer for producing an aqueous dispersible magnetic nanoparticle formulation. 
     
     
         2 . The glyceryl monooleate magnetic nanoparticle formulation as claimed in  claim 1 , further comprising one or more therapeutic agents or a functional group. 
     
     
         3 . The glyceryl monooleate magnetic nanoparticle formulation as claimed in  claim 2 , wherein said therapeutic agent is selected from hydrophobic and hydrophilic agents. 
     
     
         4 . A method for preparing glyceryl monooleate (GMO) magnetic nanoparticles (MNPs) formulation comprising:
 heating a mixture of Fe (III) and Fe (II) with constant stirring under N 2  atmosphere;   adding ammonium hydroxide to the said mixture;   adding glyceryl monooleate (GMO) to the suspension drop wise;   subjecting the mixture to the step of stirring under N 2  atmosphere;   washing the formulation several times with different solvents to wash the excess glyceryl monooleate (GMO); and   subjecting the washed formulation to the step of lyophilization to yield powder form.   
     
     
         5 . The method as claimed in  claim 4 , wherein the mixture is heated at 80° C. 
     
     
         6 . The method as claimed in  claim 4 , wherein 12-560 μl of GMO is added to get 10-504% of GMO coated MNPs. 
     
     
         7 . The method as claimed in  claim 4 , wherein the mixture is stirred for 20 minutes at 80° C. under N 2  atmosphere to evaporate excess ammonia from the formulation. 
     
     
         8 . The method as claimed in  claim 4 , wherein the solvent is ethyl acetate:acetone (70:30). 
     
     
         9 . The method as claimed in  claim 4 , wherein the formulation is sonicated for 2 minutes at 55 watt. 
     
     
         10 . The method as claimed in  claim 4 , wherein the step of washing with different solvents further comprises centrifugation for 20 minutes at 10° C. at 20,000 rpm. 
     
     
         11 . The method as claimed in  claim 4 , wherein said solvents are selected from acetone, ethyl acetate, diethyl ether, chloroform, and mixtures of different solvents in different ratios like ethyl acetate:acetone (50:50 and 70:30). 
     
     
         12 . The method as claimed in  claim 4 , wherein the step of washing is repeated for 3 times. 
     
     
         13 . The GMO-MNP formulation as claimed in  claim 1 , wherein the glyceryl monooleate (GMO) magnetic nanoparticles (MNPs) formulation is a carrier for a hydrophilic or hydrophobic drug. 
     
     
         14 . The GMO-MNP formulation as claimed in  claim 1  used in biomedical field such as cell separation. 
     
     
         15 . The GMO-MNP formulation as claimed in  claim 2 , wherein the functional group is attached to one of a protein or a targeting moiety.