US2012055793A1PendingUtilityA1

Emulsions of Ionic Liquids

52
Assignee: BRYNING ZBIGNIEW TPriority: May 23, 2003Filed: Oct 7, 2011Published: Mar 8, 2012
Est. expiryMay 23, 2023(expired)· nominal 20-yr term from priority
Inventors:Zbigniew T. Bryning
B01F 33/3031B01F 23/411
52
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Claims

Abstract

The present teachings provide emulsions using ionic liquids for separation of biomolecules and related methods, compositions, and devices.

Claims

exact text as granted — not AI-modified
1 . A composition comprising:
 an emulsion, the emulsion comprising
 emulsion droplets dispersed within an ionic liquid that forms a continuous phase, the emulsion droplets comprising (i) a solvent that is immiscible with the ionic liquid, and (ii) dissolved biomolecules that are in a charged state. 
   
     
     
         2 - 4 . (canceled) 
     
     
         5 . The composition of  claim 1 , wherein a voltage differential is present across the emulsion droplets. 
     
     
         6 - 7 . (canceled) 
     
     
         8 . The composition of  claim 1 , wherein the dissolved biomolecules are negatively charged. 
     
     
         9 . The composition of  claim 1 , wherein the solvent is aqueous. 
     
     
         10 . The composition of  claim 9 , wherein the aqueous solvent is a buffer. 
     
     
         11 . The composition of  claim 1 , wherein the solvent is a non-polar organic solvent. 
     
     
         12 . The composition of  claim 1 , wherein the ionic liquid is hydrophilic. 
     
     
         13 . The composition of  claim 1 , wherein the ionic liquid is hydrophobic. 
     
     
         14 . The composition of  claim 1 , wherein the dissolved biomolecules are concentrated towards one end in one or more droplets. 
     
     
         15 . The composition of  claim 14 , wherein the dissolved biomolecules are concentrated at the interface between the droplets and the ionic liquid. 
     
     
         16 . The composition of  claim 1 , wherein the biomolecules are found in samples of bodily fluids. 
     
     
         17 . The composition of  claim 1 , wherein different droplets within the continuous phase predominantly contain different kinds of biomolecules. 
     
     
         18 . The composition of  claim 17 , comprising a first population of droplets predominantly containing a first kind of biomolecule from a second population of droplets predominantly containing a second different kind of biomolecule, where the first population is concentrated within a different portion of the continuous phase than the second portion. 
     
     
         19 . The composition of  claim 1 , wherein different droplets within the continuous phase contain different concentrations of a biomolecule. 
     
     
         20 . The composition of  claim 17 , wherein droplets containing a first concentration of a biomolecule are generally concentrated within a different portion of the continuous phase from droplets containing a different concentration of biomolecule. 
     
     
         21 . The composition of  claim 1 , wherein the biomolecules comprise nucleic acid. 
     
     
         22 . The composition of  claim 1 , wherein the biomolecules comprise protein.

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