Isolated polynucleotides and polypeptides relating to loci underlying resistance to soybean cyst nematode and soybean sudden death syndrome and methods employing same

Abstract

Soybean cyst nematode and soybean sudden death syndrome resistance genes, soybean cyst nematode and soybean sudden death syndrome resistant plant lines, and methods of breeding and engineering same.

1 - 10 . (canceled) 
     
     
         11 . An isolated and purified nucleic acid molecule encoding a biologically active SCN/SDS resistance polypeptide and further comprising an isolated soybean rhg1 and SDS resistance gene, said gene capable of conveying  Heterodera glycines -infestation resistance,  Fusarium solani -infection resistance, or both  Heterodera glycines -infestation resistance and  Fusarium solani -infection resistance to a non-resistant soybean germplasm, said gene located within a quantitative trait locus mapping to linkage group G and mapped by genetic markers of SEQ ID NOs:1-6, said gene located along said quantitative trait locus between said markers, wherein said SCN/SDS resistance polypeptide has at least 95% sequence identity to SEQ ID NO 14. 
     
     
         12 . The nucleic acid molecule of  claim 11 , wherein the encoded polypeptide comprises a soybean SCN/SDS resistance polypeptide. 
     
     
         13 . (canceled) 
     
     
         14 . The nucleic acid molecule of  claim 11 , further defined as comprising:
 (a) the nucleotide sequence of SEQ ID NO:13 or   (b) a nucleotide sequence that has at least 95% sequence identity to SEQ ID NO:13.   
     
     
         15 - 16 . (canceled) 
     
     
         17 . The nucleic acid molecule of  claim 11 , further defined as a DNA segment. 
     
     
         18 . The nucleic acid molecule of  claim 11 , further defined as positioned under the control of a promoter. 
     
     
         19 . The nucleic acid molecule of  claim 18 , wherein said DNA segment and promoter are operationally inserted into a recombinant vector. 
     
     
         20 . A recombinant host cell comprising the nucleic acid molecule of  claim 11 . 
     
     
         21 . A transgenic plant having incorporated into its genome a nucleic acid molecule of  claim 11 , the nucleic acid molecule being present in said genome in a copy number effective to confer expression in the plant of an SCN/SDS resistance polypeptide. 
     
     
         22 . Plant seeds, parts, or progeny of a plant as claimed in  claim 20 . 
     
     
         23 . An isolated and purified nucleic acid molecule encoding a biologically active SCN/SDS resistance polypeptide and further comprising an isolated soybean Rhg4 gene, said gene capable of conveying  Heterodera glycines -infestation resistance to a non-resistant soybean germplasm, said gene located within a quantitative trait locus mapping to linkage group A2 and mapped by the AFLP markers of SEQ ID NOs:7-12, said gene located along said quantitative trait locus between said markers. 
     
     
         24 . The isolated nucleic acid molecule of  claim 23 , further comprising:
 (a) the nucleotide sequence of any one of SEQ ID NOs:16-19; or   (b) a nucleotide sequence substantially similar to any one of SEQ ID NOs:16-19.   
     
     
         25 . A transgenic plant comprising the isolated soybean Rhg4 gene of  claim 23 . 
     
     
         26 . Seeds, parts or progeny of a plant as claimed in  claim 25 . 
     
     
         27 - 80 . (canceled)