Compositions for treating nervous system conditions

Abstract

Compositions for treating nervous system conditions. In at least one embodiment of a stem cell conditioned medium of the present disclosure, the stem cell comprises a cell culture supernatant containing at least one factor capable of exerting effective neuroprotection to treat a mammalian neural injury or insult, the cell culture supernatant produced by culturing at least one mammalian adipose stem cell to produce the at least one factor.

1 . A stem cell conditioned medium, comprising:
 a cell culture supernatant containing at least one factor capable of exerting effective neuroprotection to treat a mammalian neural injury or insult, the cell culture supernatant produced by culturing at least one mammalian adipose stem cell to produce the at least one factor.   
     
     
         2 . The stem cell conditioned medium of  claim 1 , wherein the cell culture supernatant is produced by culturing at least one mammalian adipose stem cell in a first cell culture medium and later removing some or all of the first cell culture medium and further culturing the at least one mammalian adipose stem cell in a second cell culture medium. 
     
     
         3 . The stem cell conditioned medium of  claim 2 , wherein the second cell culture medium comprises a growth factor-free basal medium. 
     
     
         4 . The stem cell conditioned medium of  claim 2 , wherein the first cell culture medium comprises a first amount of growth factor, wherein the second cell culture medium comprises a second amount of growth factor, and wherein the second amount is less than the first amount. 
     
     
         5 . The stem cell conditioned medium of  claim 2 , wherein the cell culture supernatant is collected after the at least one mammalian adipose stem cell has been cultured in the second cell culture medium. 
     
     
         6 . The stem cell conditioned medium of  claim 1 , wherein the at least one factor is selected from the group consisting of at least one angoigenic factor and at least one antiapoptotic factor. 
     
     
         7 . The stem cell conditioned medium of  claim 1 , wherein the at least one factor capable of exerting effective neuroprotection is capable of protecting against mammalian neuronal cell death. 
     
     
         8 . The stem cell conditioned medium of  claim 1 , wherein the cell culture supernatant is concentrated to a concentration selected from the group of at least 50-fold, between 50-fold and 100-fold, at least 100-fold, between 100-fold and 250-fold, and at least 250-fold. 
     
     
         9 . The stem cell conditioned medium of  claim 1 , wherein the cell culture supernatant is fractionated to remove substances less than a specified kDa range selected from the group consisting of less than about 5 kDa, less than about 10 kDa, less than about 20 kDa, less than about 30 kDa, less than about 40 kDa, less than about 50 kDa, and greater than about 50 kDa. 
     
     
         10 . The stem cell conditioned medium of  claim 1 , wherein the cell culture supernatant is acted upon using a process selected from centrifugation, concentration, fractionation, lyophilization, and freeze-drying. 
     
     
         11 . The stem cell conditioned medium of  claim 1 , wherein the supernatant comprises at least one chemical secretion from the cultured at least one mammalian adipose stem cell. 
     
     
         12 . The stem cell conditioned medium of  claim 1 , wherein the at least one factor comprises at least two different factors having a synergistic effect on a target cell and/or organ. 
     
     
         13 . The stem cell conditioned medium of  claim 1 , wherein the at least one factor comprises a plurality of factors. 
     
     
         14 . The stem cell conditioned medium of  claim 2 , wherein the at least one mammalian stem cell is cultured in the second cell culture medium under a condition selected from the group consisting of a normoxic condition and a hypoxic condition. 
     
     
         15 . The stem cell conditioned medium of  claim 1 , wherein the mammalian neural injury or insult that at least one factor capable is of exerting effective neuroprotection to treat is selected from the group consisting of a chronic injury, an acute injury, a central nervous system injury, a peripheral nervous system injury, and a neurodegenerative disorder. 
     
     
         16 . The stem cell conditioned medium of  claim 1 , wherein the mammalian neural injury or insult is selected from the group consisting of Parkinson's disease, Alzheimer's disease, amyotrophic lateral sclerosis, multiple sclerosis, cerebral palsy, peripheral neuropathy, Huntington's disease, epilepsy, encephalomyelitis, encephalitis, inflammation, a spinal cord injury, a brain injury, and cerebral hypoxia. 
     
     
         17 . A stem cell conditioned medium, comprising:
 a cell culture supernatant containing at least one factor capable of exerting effective neuroprotection to treat a mammalian neural injury or insult, the cell culture supernatant produced by culturing at least one mammalian adipose stem cell in a first cell culture medium, later removing some or all of the first cell culture medium and further culturing the at least one mammalian adipose stem cell in a second cell culture medium, and fractionating the cell culture supernatant to remove substances less than a specified kDa range to produce the at least one factor.   
     
     
         18 . A stem cell conditioned medium, comprising:
 a supernatant containing at least one factor, the supernatant obtained by culturing at least one mammalian adipose stem cell in a first cell culture medium, switching the cell culture medium to a substantially growth factor-free second cell culture medium, and collecting the supernatant containing the at least one factor after switching the cell culture medium to the growth factor-free second cell culture medium.   
     
     
         19 . The stem cell conditioned medium of  claim 18 , wherein the cell culture supernatant is concentrated to a concentration selected from the group of at least 50-fold, between 50-fold and 100-fold, at least 100-fold, between 100-fold and 250-fold, and at least 250-fold. 
     
     
         20 . The stem cell conditioned medium of  claim 18 , wherein the cell culture supernatant is fractionated to remove substances less than a specified kDa range selected from the group consisting of less than about SkDa, less than about 10 kDa, less than about 20 kDa, less than about 30 kDa, less than about 40 kDa, less than about 50 kDa, and greater than about 50 kDa.