Method of mass producing growth factor using adipose derived adult stem cells

Abstract

The present invention relates to a method for producing large amounts of human growth factors from human adipose-derived stem cells. More specifically, the invention provides a method capable of synthesizing human growth factors in significantly large amounts by culturing adipose-derived stem cells extracted from human adipose cells in suitable media and conditions. Also, stem cell culture media produced according to the method of the invention, and human growth factors isolated from the culture media, can be advantageously used as raw materials for drugs and cosmetics.

1 . A cosmetic composition having a functionality and containing an adipose-derived adult stem cell culture medium obtained by a method comprising the steps of:
 (i) isolating adipose-derived adult stem cells extracted from mammalian adipose cells;   (ii) optionally culturing the stem cells in a serum medium, and then subculturing the stem cells in a serum-free medium;   (iii) applying, to the adipose-derived stem cells, at least one physical stimulation selected from among low-oxygen culture, UV irradiation, nutrient deficiency and mechanical friction; and   (iv) optionally adding, to the culture media, one or more vitamins selected from among vitamin A, vitamin B, vitamin C and vitamin D,   wherein step (iii) and step (iv) are performed in conditions in which the highest production of the human growth factors occurs.   
     
     
         2 . The cosmetic composition of  claim 1 , wherein the adipose-derived adult stem cell culture medium contains human growth factors. 
     
     
         3 . The cosmetic composition of  claim 1 , wherein the adipose-derived adult stem cell culture medium contains a human basic fibroblast growth factor (bFGF). 
     
     
         4 . The cosmetic composition of  claim 1 , wherein the adipose-derived adult stem cell culture medium contains a human vascular endothelial growth factor (VEGF). 
     
     
         5 . The cosmetic composition of  claim 1 , wherein the adipose-derived adult stem cell culture medium contains a human transforming growth factor-beta (TGF-β). 
     
     
         6 . The cosmetic composition of  claim 1 , wherein the functionality is an anti-wrinkle or anti-aging activity. 
     
     
         7 . A cosmetic composition for anti-wrinkle and/or anti-aging, which comprises a culture medium of stem cells, in which the culture medium is obtained by a method comprising the steps of:
 (i) isolating adipose-derived adult stem cells extracted from mammalian adipose cells; and   (ii) culturing the stem cells in a serum medium, and then subculturing the stem cells in a serum-free medium.