Prevention and treatment of blood coagulation-related disases

Abstract

Provided herein is an animal having a persistent hypercoagulable state by implanting a cell, for example a tumor cell, in which the gene of human tissue factor is implanted to an experimental animal such as a mouse and then growing the cell, thereby persistently supplying human tissue factor to the experimental animal. This animal model is useful for research and development of therapeutic agents for diseases having a persistent hypercoagulable state. Also provided are preventive or therapeutic agents for diseases having a persistent hypercoagulable state, a hypercoagulable state resulting from infections, venous thrombosis, arterial thrombosis, and diseases resulting from the hypertrophy of vascular media, the agent comprising an antibody against human tissue factor (human TF) as an active ingredient.

1 . An experimental animal having implanted therein an animal cell to which the gene encoding human tissue factor (TF) or part thereof has been inserted and which is capable of expressing said gene, said animal being a non-human animal in which a hypercoagulable state persists for a long period of time. 
     
     
         2 . The animal according to  claim 1  wherein said part of human tissue factor lacks the intracellular region. 
     
     
         3 . The animal according to  claim 1  wherein said animal cell is a mammalian cell. 
     
     
         4 . The animal according to  claim 3  wherein said mammalian cell is a human myeloma cell. 
     
     
         5 . The animal according to  claim 1  wherein said animal is a mouse. 
     
     
         6 . The animal according to  claim 1  wherein said hypercoagulable state is indicated by at least one of the phenomena comprising an increase in the plasma concentration of human tissue factor, a decrease in platelets, a decrease in fibrinogen, an increase in the concentration of soluble fibrin monomer complex, and an increase in the concentration of thrombin-antithrombin III complex. 
     
     
         7 . A method of generating the animal according to  claim 1 , wherein an animal cell to which the gene encoding human tissue factor (TF) or part thereof has been inserted and which is capable of expressing said gene is implanted to non-human animals and then an animal having a persistent hypercoagulable state is selected. 
     
     
         8 . A method of screening an anti-thrombotic agent which method comprises using the animal according to  claim 1 .