Rapid Microbial Detection and Antimicrobial Susceptibility Testing

Abstract

A method for the detection of microorganisms in a sample comprising contacting said sample with a biosensor concentration module, allowing microorganisms to grow for a first period of time and detecting growth of discrete microorganisms as an indication of the presence of said microorganisms.

1 . (canceled) 
     
     
         2 . A method of screening for an antimicrobial agent of at least one microorganism comprising:
 contacting said microorganism with a biosensor comprising:
 An concentration module; 
 At least one detection surface; and 
   concentrating said microorganisms onto said detection surface, wherein a plurality of said microorganisms bind to said detection surface in spatially discrete sites;   allowing said microorganisms to grow for a first period of time;   adding at least one candidate antimicrobial agent to said microorganisms;   allowing said microorganisms to grow for a second period of time;   detecting alterations in the growth of discrete microorganisms as compared to the absence of said candidate agent.