Expression System Of NELL Peptide

Abstract

Recombinant NELL peptides and methods of preparing the same are disclosed.

1 . A composition for inducing bone or cartilage formation comprising: an effective amount of a recombinant Nel-like molecule-1 (NELL-1) peptide comprising an amino acid sequence of SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, and SEQ ID NO:14, and a carrier, wherein the carrier comprises a physiologically acceptable carrier that forms an implant or scaffold that provides for bone or cartilage formation. 
     
     
         2 . The composition of  claim 1 , wherein the NELL-1 peptide further comprises a NELL1 or NELL2 secretory peptide. 
     
     
         3 . The composition of  claim 1 , wherein the carrier is a scaffold. 
     
     
         4 . The composition of  claim 1 , which is in a formulation for injection into a fracture site or implantation. 
     
     
         5 . The composition of  claim 1 , further comprising another agent. 
     
     
         6 . The composition of  claim 5 , wherein the another agent is a selected from the group consisting of bone morphogenetic protein (BMP), a fibroblast growth factor (FGF), a transforming growth factor-β (TGF-β), an insulin-like growth factor (IGF), a vascular endothelial growth factor (VEGF), a platelet-derived growth factor (PDGF), a parathyroid hormone (PTH), a PTH-regulated protein) PTHrp), growth/differentiation factor 5 (GDF5); and LIM mineralization proteins (LMPs).