US2012078163A1PendingUtilityA1

Method of generating connective tissue

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Assignee: GAZIT DANPriority: May 27, 2009Filed: May 27, 2010Published: Mar 29, 2012
Est. expiryMay 27, 2029(~2.9 yrs left)· nominal 20-yr term from priority
Inventors:Dan GazitGadi PelledZulma GazitNadav Kimelman-Bleich
A61L 27/3834A61L 27/24A61L 27/52A61L 27/54A61L 27/14A61L 2300/43A61P 19/00A61L 2430/02A61L 2300/414A61L 2430/10A61L 2300/252A61K 38/1875A61L 2300/258A61L 27/56
35
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Claims

Abstract

A method of generating bone or connective tissue in a subject in need thereof, the method comprising: (a) implanting a scaffold in a damaged region of the subject, the scaffold comprising a tissue growth promoting agent; and (b) enhancing uptake of the tissue growth promoting agent into cells located on the scaffold, wherein the enhancing is effected by induced poration of the cells thereby generating bone or connective tissue in the subject. Kits for generating bone or connective tissue are also provided.

Claims

exact text as granted — not AI-modified
1 . A method of generating bone or connective tissue in a subject in need thereof, the method comprising:
 (a) implanting a scaffold in a damaged region of the subject, said scaffold comprising a tissue growth promoting agent; and   (b) enhancing uptake of said tissue growth promoting agent into cells located on said scaffold, wherein said enhancing is effected by induced poration of said cells thereby generating bone or connective tissue in the subject.   
     
     
         2 . A method of generating bone or connective tissue in a subject in need thereof, the method comprising:
 (a) implanting a scaffold in a damaged region of the subject;   (b) administering to the subject a tissue growth promoting agent at a site of said implanting; and   (c) enhancing uptake of said tissue growth promoting agent into cells located on said scaffold, wherein said enhancing is effected by induced poration of said cells, thereby generating bone or connective tissue in the subject.   
     
     
         3 . The method of  claim 1 , wherein said induced portation is effected by a method selected from the group consisting of electroporation, sonoporation and laser-induced poration. 
     
     
         4 . The method of  claim 1 , further comprising seeding said cells on said scaffold prior to said implanting. 
     
     
         5 . The method of  claim 1 , wherein said cells comprise stem cells. 
     
     
         6 . The method of  claim 1 , wherein said tissue growth promoting agent is selected from the group consisting of a polynucleotide agent, a polypeptide agent, a hormone and a small molecule. 
     
     
         7 . The method of  claims 1 , wherein said tissue growth promoting agent comprises an osteogenic agent. 
     
     
         8 . The method of  claim 7 , wherein said osteogenic agent is a bone morphogenetic protein (BMP). 
     
     
         9 - 14 . (canceled) 
     
     
         15 . The method of  claim 1 , wherein said scaffold comprises collagen. 
     
     
         16 . The method of  claim 1 , wherein said induced poration is effected using needle electrodes. 
     
     
         17 . The method of  claim 1 , wherein said enhancing uptake is effected 7-12 days following said implanting. 
     
     
         18 . A kit for generating bone or connective tissue, comprising:
 (i) a scaffold;   (ii) a tissue growth promoting agent; and   (iii) instructions for generating the bone or cartilage tissue, the instructions comprising a protocol for enhancing uptake of said tissue growth promoting agent into cells via induced poration.   
     
     
         19 . (canceled) 
     
     
         20 . The kit of  claim 18 , wherein said tissue growth promoting agent is comprised in said scaffold. 
     
     
         21 . The kit of  claim 18 , wherein said scaffold comprises collagen. 
     
     
         22 . (canceled) 
     
     
         23 . The kit of  claim 18 , wherein said tissue growth promoting agent comprises an osteogenic agent. 
     
     
         24 . (canceled) 
     
     
         25 . The kit of  claim 23 , wherein said osteogenic agent is a bone morphogenetic protein (BMP). 
     
     
         26 - 27 . (canceled) 
     
     
         28 . The kit of  claim 18 , further comprising a needle electrode for performing said induced poration. 
     
     
         29 - 30 . (canceled) 
     
     
         31 . The method of  claim 2 , further comprising seeding said cells on said scaffold prior to said implanting. 
     
     
         32 . The method of  claim 2 , wherein said cells comprise stem cells. 
     
     
         33 . The method of  claim 2 , wherein said tissue growth promoting agent is selected from the group consisting of a polynucleotide agent, a polypeptide agent, a hormone and a small molecule. 
     
     
         34 . The method of  claim 2 , wherein said tissue growth promoting agent comprises an osteogenic agent. 
     
     
         35 . The method of  claim 34 , wherein said osteogenic agent is a bone morphogenetic protein (BMP). 
     
     
         36 . The method of  claim 2 , wherein said scaffold comprises collagen. 
     
     
         37 . The method of  claim 2 , wherein said induced poration is effected using needle electrodes. 
     
     
         38 . The method of  claim 2 , wherein said enhancing uptake is effected 7-12 days following said implanting.

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