US2012094834A1PendingUtilityA1

method for increasing the vigor and/or crop yield of agricultural plants under essentially non-existent pathogen pressure

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Assignee: FRANK MARKUSPriority: May 6, 2009Filed: May 3, 2010Published: Apr 19, 2012
Est. expiryMay 6, 2029(~2.8 yrs left)· nominal 20-yr term from priority
A01N 55/02A01N 43/653A01N 59/20A01N 47/30A01N 63/22C05G 3/60Y02A50/30
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Claims

Abstract

A method for increasing the vigor and/or crop yield of agricultural plants under essentially non-existent pathogen pressure, wherein the plants, the plant propagules, the seed of the plants and/or the locus where the plants are growing or are intended to grow are treated with an effective amount of a composition comprising a) the Bacillus subtilis strain with NRRL Accession No. B-21661 or a cell-free extract thereof, and/or a mutant of this strain or extract having all the identifying characteristics of the respective strain or extract as component (I), and b) optionally at least one chemical compound as component (II), selected from the active compound groups A) to J): A) strobilurins; B) carboxamides; C) azoles; D) heterocyclic compounds; E) carbamates; F) other active substances; G) growth regulators; H) herbicides; J) insecticides.

Claims

exact text as granted — not AI-modified
1 - 15 . (canceled) 
     
     
         16 . A method for increasing the vigor and/or crop yield of agricultural plants under essentially non-existent pathogen pressure, wherein the plants, the plant propagules, the seed of the plants and/or the locus where the plants are growing or are intended to grow are treated with an effective amount of a composition comprising
 a) the  Bacillus subtilis  strain with NRRL Accession No. B-21661 or a cell-free extract thereof, and/or a mutant of this strain or extract having all the identifying characteristics of the respective strain or extract as component (I), and   b) optionally at least one chemical compound as component (II), selected from the active compound groups A) to J):
 A) a strobilurin selected from the group consisting of azoxystrobin, dimoxy-strobin, enestroburin, fluoxastrobin, kresoxim-methyl, metominostrobin, orysastrobin, picoxystrobin, pyraclostrobin, pyribencarb, trifloxystrobin, 2-(2-(6-(3-chloro-2-methyl-phenoxy)-5-fluoro-pyrimidin-4-yloxy)-phenyl)-2-methoxyimino-N-methyl-acetamide, 3-methoxy-2-(2-(N-(4-methoxy-phenyl)-cyclopropane-carboximidoylsulfanylmethyl)-phenyl)-acrylic acid methyl ester, methyl (2-chloro-5[1-(3-methylbenzyloxyimino)-ethyl]benzyl)carbamate and 2 (2-(3-(2,6-dichlorophenyl)-1-methyl-allylideneaminooxy-methyl)-phenyl)-2-methoxyimino-N methyl-acetamide; 
 B) a carboxamide selected from the group consisting of
 carboxanilides: benalaxyl, benalaxyl-M, benodanil, bixafen, boscalid, carboxin, fenfuram, fenhexamid, flutolanil, furametpyr, isopyrazam, isotianil, kiralaxyl, mepronil, metalaxyl, metalaxyl-M (mefenoxam), ofurace, oxadixyl, oxycarboxin, penthiopyrad, sedaxane, tecloftalam, thifluzamide, tiadinil, 2-amino-4-methyl-thiazole-5-carboxanilide, 2 chloro-N (1,1,3-trimethyl-indan-4-yl)-nicotinamide, N-(3′,4′,5′ trifluorobiphenyl-2 yl)-3-difluoromethyl-1-methyl-1H-pyrazole-4 carboxamide, N-(4′-trifluoromethylthiobiphenyl-2-yl)-3 difluoromethyl-1-methyl-1H-pyrazole-4-carboxamide, N (2-(1,3 dimethyl-butyl)-phenyl)-1,3-dimethyl-5-fluoro-1H-pyrazole-4-carboxamide and N-(2-(1,3,3-trimethyl-butyl)-phenyl)-1,3-dimethyl-5-fluoro-1H-pyrazole-4 carboxamide; 
 a carboxylic morpholide selected from the group consisting of dimethomorph, flumorph, pyrimorph; 
 benzoic acid amides: flumetover, fluopicolide, fluopyram, zoxamide, and N-(3-Ethyl-3,5,5-trimethyl-cyclohexyl)-3-formylamino-2-hydroxy-benzamide; and 
 another carboxamide selected from the group consisting of carpropamid, dicyclomet, mandiproamid, oxy-tetracyclin, silthiofarm and N-(6-methoxy-pyridin-3-yl)cyclopropane-carboxylic acid amide; 
 
 C) an azole selected from the group consisting of
 a triazole selected from the group consisting of azaconazole, bitertanol, bromuconazole, cyproconazole, difenoconazole; diniconazole, diniconazole-M, epoxiconazole, fenbuconazole, fluquinconazole, flusilazole, flutriafol, hexaconazole, imibenconazole, ipconazole, metconazole, myclobutanil, oxpocona-zole, paclobutrazole, penconazole, propiconazole, prothio-conazole, simeconazole, tebuconazole, tetraconazole, triadimefon, triadimenol, triticonazole, uniconazole, 1-(4-chloro-phenyl)-2-([1,2,4]triazol-1-yl)-cycloheptanol; 
 an imidazole selected from the group consisting of cyazofamid, imazalil, pefurazoate, prochloraz, triflumizol; 
 a benzimidazole selected from the group consisting of benomyl, carbendazim, fuberidazole, and thiabendazole; and 
 another compound selected from the group consisting of ethaboxam, etridiazole, hymexazole and 2-(4-chloro-phenyl)-N-[4-(3,4-dimethoxy-phenyl)-isoxazol-5-yl]-2-prop-2-ynyloxy-acetamide; 
 
 D) a heterocyclic compound selected from the group consisting of
 a pyridine selected from the group consisting of fluazinam, pyrifenox, 3-[5-(4-chloro-phenyl)-2,3-dimethyl-isoxazolidin-3 yl]-pyridine, 3-[5-(4-methyl-phenyl)-2,3-dimethyl-isoxazolidin-3-yl]-pyridine, 2,3,5,6-tetra-chloro-4-methanesulfonyl-pyridine, 3,4,5-trichloropyridine-2,6-di-carbonitrile, N-(1-(5-bromo-3-chloro-pyridin-2-yl)-ethyl)-2,4-dichloronicotinamide, and N [(5 bromo-3-chloro-pyridin-2-yl)-methyl]-2,4-dichloro-nicotinamide; 
 a pyrimidine selected from the group consisting of bupirimate, cyprodinil, diflumetorim, fenarimol, ferimzo-ne, mepanipyrim, nitrapyrin, nuarimol, and pyrimethanil; 
 triforine; 
 a pyrrole selected from the group consisting of fenpiclonil and fludioxonil; 
 a morpholine selected from the group consisting of aldimorph, dodemorph, dodemorph-acetate, fenpropi-morph, and tridemorph; 
 fenpropidin; 
 a dicarboximide selected from the group consisting of fluoroimid, iprodione, procymidone, and vinclozolin; 
 a non-aromatic 5-membered heterocycle selected from the group consisting of famoxadone, fenamidone, flutianil, octhilinone, probenazole, and 5-amino-2-isopropyl-3-oxo-4-ortho-tolyl-2,3-dihydro-pyrazole-1 carbothioic acid S-allyl ester; and 
 another compound selected from the group consisting of acibenzolar-5-methyl, amisulbrom, anilazin, blasticidin-S, captafol, captan, chinomethionat, dazomet, debacarb, diclomezine, difenzoquat, difenzoquat-methylsulfate, fenoxanil, Folpet, oxolinic acid, piperalin, proquinazid, pyroquilon, quinoxyfen, triazoxide, tricy-clazole, 2-butoxy-6-iodo-3-propylchromen-4-one, 5-chloro-1 (4,6-dimethoxy-pyrimidin-2-yl)-2-methyl-1H-benzoimidazole, 5 chloro-7 (4-methylpiperidin-1-yl)-6-(2,4,6-trifluorophenyl)-[1,2,4]triazolo-[1,5 a]pyrimidine and 5-ethyl-6 octyl-[1,2,4]triazolo[1,5-a]pyrimidine-7 ylamine; 
 
 E) a carbamate selected from the group consisting of
 a thio- or a dithiocarbamate selected from the group consisting of ferbam, mancozeb, maneb, metam, methasulphocarb, metiram, propineb, thiram, zineb, and ziram; 
 a carbamate selected from the group consisting of benthiavalicarb, diethofencarb, iprovalicarb, propamo-carb, propamocarb hydrochlorid, valiphenal and N-(1-(1-(4-cyano-phenyl)ethanesulfonyl)-but-2-yl) carbamic acid-(4-fluorophenyl)ester; 
 
 F) another active substance selected from the group consisting of
 a guanidine selected from the group consisting of guanidine, dodine, dodine free base, guazatine, guaza-tine-acetate, iminoctadine, iminoctadine-triacetate, and iminoctadine-tris(albesilate); 
 an antibiotic selected from the group consisting of kasugamycin, kasugamycin hydrochloride-hydrate, streptomycin, polyoxine, validamycin A, streptomycin; 
 a nitrophenyl derivate selected from the group consisting of binapacryl, dinobuton, dinocap, nitrthal-isopropyl, tecnazen, organometal compounds: fentin salts, such as fentin-acetate, fentin chloride or fentin hydroxide; 
 a sulfur-containing heterocyclyl compound selected from the group consisting of dithianon and isoprothiolane; 
 an organophosphorus compound selected from the group consisting of edifenphos, fosetyl, fosetyl-aluminum, iprobenfos, phosphorous acid and its salts, pyrazophos, and tolclofos-methyl; 
 an organochlorine compound selected from the group consisting of chlorothalonil, dichlofluanid, dichloro-phen, flusulfamide, hexachlorobenzene, pencycuron, pentachlor-phenole and its salts, phthalide, quintozene, thiophanate-methyl, and tol-ylfluanid, N-(4-chloro-2-nitro-phenyl)-N-ethyl-4-methyl-benzenesulfonamide; 
 an inorganic active substance, selected from the group consisting of Bordeaux mixture, copper acetate, copper hydroxide, copper oxychloride, basic copper sulfate, sulfur; 
 another compound selected from the group consisting of biphenyl, bronopol, cyflufenamid, cymoxanil, diphenylamin, metrafenone, mildiomycin, oxin-copper, prohexadione-calcium, spiroxamine, tolylfluanid, N-(cyclopropylmethoxyimino-(6-difluoro-methoxy-2,3-difluoro-phenyl)-methyl)-2-phenyl acetamide, N-(4-(4-chloro-3-trifluoromethyl-phenoxy)-2,5-dimethyl-phenyl)-N-ethyl-N methyl formamidine, N′ (4-(4-fluoro-3-trifluoromethyl-phenoxy)-2,5-dimethyl-phenyl)-N-ethyl-N-methyl formamidine, N′-(2-methyl-5-trifluoromethyl-4-(3-trimethylsilanyl-propoxy)-phenyl)-N-ethyl-N-methyl formamidine, N′-(5-difluoromethyl-2 methyl-4-(3-tri-methylsilanyl-propoxy)-phenyl)-N-ethyl-N-methyl formamidine, 2-{1-[2-(5-methyl-3-trifluoromethyl-pyrazole-1-yl)-acetyl]-piperidin-4-yl}-thiazole-4-carboxylic acid methyl-(1,2,3,4-tetrahydro-naphthalen-1-yl)-amide, 2-{1-[2-(5-methyl-3-trifluoromethyl-pyrazole-1-yl)-acetyl]-piperidin-4-yl}-thiazole-4-carboxylic acid methyl-(R)-1,2,3,4-tetrahydro-naphthalen-1-yl-amide, acetic acid 6-tert-butyl-8-fluoro-2,3-dimethyl-quinolin-4-yl ester and methoxy-acetic acid 6-tert-butyl-8-fluoro-2,3-dimethyl-quinolin-4-yl ester, fentin acetate, fentin chloride, and fentin hydroxide; 
 
 G) a plant growth regulator (PGRs) selected from the group consisting of abscisic acid, amidochlor, ancymidol, 6-benzylaminopurine, brassinolide, butralin, chlormequat (chlormequat chloride), choline chloride, cyclanilide, daminozide, dikegulac, dimethipin, 2,6-dimethylpuridine, ethephon, flumetralin, flurprimidol, fluthiacet, forchlorfenuron, gibberellic acid, inabenfide, indole-3-acetic acid, maleic hydrazide, mefluidide, mepiquat (mepiquat chloride), naphthaleneacetic acid, N 6 benzyladenine, paclobutrazol, prohexadione (prohexadione-calcium), prohydrojasmon, thidiazuron, triapenthenol, tributyl phosphorotrithioate, 2,3,5 tri iodobenzoic acid, trinexapac-ethyl and uniconazole; 
 H) a herbicide selected from the group consisting of
 an acetamide selected from the group consisting of acetochlor, alachlor, butachlor, dimethachlor, dimethenamid, flufenacet, mefenacet, metolachlor, metazachlor, napropamide, naproanilide, pethoxamid, pretilachlor, propachlor, and thenylchlor; 
 an amino acid derivative selected from the group consisting of bilanafos, glufosinate, and sulfosate; 
 an aryloxyphenoxypropionate selected from the group consisting of clodinafop, cyhalofop-butyl, fenoxaprop, fluazifop, haloxyfop, metamifop, propaquizafop, quizalofop, quizalo-fop-P-tefuryl; 
 Bipyridyls: diquat, and paraquat; 
 a (thio)carbamate selected from the group consisting of asulam, butylate, carbetamide, desmedipham, dimepiperate, eptam (EPTC), esprocarb, molinate, orbencarb, phenmedipham, prosulfocarb, pyributicarb, thiobencarb, and triallate; 
 a cyclohexanedione selected from the group consisting of butroxydim, clethodim, cycloxydim, profoxydim, sethoxydim, tepraloxydim, and tralkoxydim; 
 a dinitroaniline selected from the group consisting of benfluralin, ethalfluralin, oryzalin, pendimethalin, prodiamine, and trifluralin; 
 a diphenyl ether selected from the group consisting of acifluorfen, aclonifen, bifenox, diclofop, ethoxyfen, fomesafen, lactofen, and oxyfluorfen; 
 a hydroxybenzonitrile selected from the group consisting of bomoxynil, dichlobenil, and ioxynil; 
 an imidazolinone selected from the group consisting of imazamethabenz, imazamox, imazapic, imazapyr, imazaquin, and imazethapyr; 
 a phenoxy acetic acid selected from the group consisting of clomeprop, 2,4-dichlorophenoxyacetic acid (2,4-D), 2,4-DB, dichlorprop, MCPA, MCPA-thioethyl, MCPB, Mecoprop; 
 pyrazines: chloridazon, flufenpyr-ethyl, fluthiacet, norflurazon, and pyri-date; 
 a pyridine selected from the group consisting of aminopyralid, clopyralid, diflufenican, dithiopyr, fluridone, fluoroxypyr, picloram, picolinafen, and thiazopyr; 
 a sulfonyl urea selected from the group consisting of amidosulfuron, azimsulfuron, bensulfuron, chlorimuron-ethyl, chlorsulfuron, cinosulfuron, cyclosulfamuron, ethoxysulfuron, flazasulfuron, flucetosulfuron, flupyrsulfuron, foramsulfuron, halosulfuron, imazosulfuron, iodosulfuron, mesosulfuron, metsulfuron-methyl, nicosulfuron, oxasulfuron, primisulfuron, prosulfuron, pyrazosulfuron, rimsulfuron, sulfometuron, sulfosulfuron, thifensulfuron, triasulfuron, tribenuron, trifloxysulfuron, triflusulfuron, tritosulfuron, and 1 ((2-chloro-6-propyl-imidazo[1,2-b]pyridazin-3-yl)sulfonyl)-3-(4,6-dimethoxy-pyrimidin-2-yl)urea; 
 a triazine selected from the group consisting of ametryn, atrazine, cyanazine, dimethametryn, ethiozin, hexazinone, metamitron, metribuzin, prometryn, simazine, terbuthylazine, terbutryn, and triaziflam; 
 a urea compound selected from the group consisting of chlorotoluron, daimuron, diuron, fluometuron, isoproturon, linuron, methabenzthiazuron, tebuthiuron; 
 an acetolactate synthase inhibitor selected from the group consisting of bispyribac-sodium, cloransulam-methyl, diclosulam, florasulam, flucarbazone, flumetsulam, metosulam, ortho-sulfamuron, penoxsulam, propoxycarbazone, pyribambenz-propyl, pyribenzoxim, pyriftalid, pyriminobac-methyl, pyrimisulfan, pyrithiobac, pyroxasulfone, and pyroxsulam; 
 another compound selected from the group consisting of amicarbazone, aminotriazole, anilofos, beflubutamid, bena-zolin, bencarbazone, benfluresate, benzofenap, bentazone, benzo-bicyclon, bromacil, bromobutide, butafenacil, butamifos, cafenstrole, carfentrazone, cinidon-ethlyl, chlorthal, cinmethylin, clomazone, cumyluron, cyprosulfamide, dicamba, difenzoquat, diflufenzopyr,  Drechslera monoceras , endothal, ethofumesate, etobenzanid, fentrazamide, flumiclorac-pentyl, flumioxazin, flupoxam, fluorochloridone, flurtamone, indanofan, isoxaben, isoxaflutole, lenacil, propanil, propyzamide, quinclorac, quinmerac, mesotrione, methyl arsonic acid, naptalam, oxadiargyl, oxadiazon, oxaziclomefone, pentoxazone, pinoxaden, pyraclonil, pyraflufen-ethyl, pyrasulfotole, pyrazoxyfen, pyrazolynate, quinoclamine, saflufenacil, sulcotrione, sulfentrazone, terbacil, tefuryltrione, tembotrione, thiencarbazone, topramezone, 4-hydroxy-3-[2-(2-methoxy-ethoxymethyl)-6-trifluoromethyl-pyridine-3-carbonyl]-bicyclo[3.2.1]oct-3-en-2-one, (3-[2-chloro-4-fluoro-5-(3-methyl-2,6-dioxo-4-trifluoromethyl-3,6-dihydro-2H-pyrimidin-1-yl)-phenoxy]-pyridin-2-yloxy)-acetic acid ethyl ester, 6-amino-5-chloro-2-cyclopropyl-pyrimidine-4-carboxylic acid methyl ester, 6-chloro-3-(2-cyclopropyl-6-methyl-phenoxy)-pyridazin-4-ol, 4-amino-3-chloro-6-(4-chloro-phenyl)-5-fluoro-pyridine-2-carboxylic acid, 4-amino-3-chloro-6-(4-chloro-2-fluoro-3-methoxy-phenyl)-pyridine-2-carboxylic acid methyl ester, and 4-amino-3-chloro-6-(4-chloro-3-dimethylamino-2-fluoro-phenyl)-pyridine-2-carboxylic acid methyl ester; and 
 
 J) an insecticide selected from the group consisting of
 an organo(thio)phosphate selected from the group consisting of acephate, azamethiphos, azinphos-methyl, chlorpyrifos, chlorpyrifos-methyl, chlorfenvinphos, diazinon, dichlorvos, dicrotophos, dimethoate, disulfoton, ethion, fenitrothion, fenthion, isoxathion, malathion, methamidophos, methidathion, methyl-parathion, mevinphos, monocrotophos, oxydemeton-methyl, paraoxon, parathion, phenthoate, phosalone, phosmet, phos-phamidon, phorate, phoxim, pirimiphos-methyl, profenofos, prothiofos, sulprophos, tetrachlorvinphos, terbufos, triazophos, and trichlorfon; 
 a carbamate selected from the group consisting of alanycarb, aldicarb, bendiocarb, benfuracarb, carbaryl, carbofuran, carbosulfan, fenoxycarb, furathiocarb, methiocarb, methomyl, oxamyl, pirimicarb, propoxur, thiodicarb, and triazamate; 
 a pyrethroid selected from the group consisting of allethrin, bifenthrin, cyfluthrin, cyhalothrin, cyphenothrin, cypermethrin, alpha-cypermethrin, beta-cypermethrin, zeta-cypermethrin, deltamethrin, esfenvalerate, etofenprox, fenpropathrin, fenvalerate, imiprothrin, lambda-cyhalothrin, permethrin, prallethrin, pyrethrin I and II, resmethrin, silafluofen, tau-fluvalinate, tefluthrin, tetramethrin, tralomethrin, transfluthrin, profluthrin, and dimefluthrin; 
 an insect growth regulator selected from the group consisting of a) a chitin synthesis inhibitor wherein said chitin synthesis inhibitor is a benzoylurea selected from the group consisting of chlorfluazuron, cyramazin, diflubenzuron, flucycloxuron, flufenoxuron, hexaflumuron, lufenuron, novaluron, teflubenzuron, tri-flumuron; buprofezin, diofenolan, hexythiazox, etoxazole, and clofentazine; b) an ecdysone antagonist selected from the group consisting of halofenozide, methoxyfenozide, tebufenozide, and azadirachtin; c) a juvenoid selected from the group consisting of pyriproxyfen, methoprene, and fenoxycarb; or d) a lipid biosynthesis inhibitor selected from the group consisting of spirodiclofen, spi-romesifen, and spirotetramat; 
 a nicotinic receptor agonist/antagonist compound selected from the group consisting of clothianidin, di-notefuran, imidacloprid, thiamethoxam, nitenpyram, acetamiprid, thi-acloprid, and 1-(2-chloro-thiazol-5-ylmethyl)-2-nitrimino-3,5-dimethyl-[1,3,5]triazinane; 
 a GABA antagonist compound selected from the group consisting of endosulfan, ethiprole, fipronil, vaniliprole, pyrafluprole, pyriprole, and 5-amino-1-(2,6-dichloro-4-methyl-phenyl)-4-sulfinamoyl-1H pyrazole-3-carbothioic acid amide; 
 a macrocyclic lactone insecticide selected from the group consisting of abamectin, emamectin, milbemec-tin, lepimectin, spinosad, and spinetoram; 
 a mitochondrial electron transport inhibitor (METI) I acaricide selected from the group consisting of fenazaquin, pyridaben, tebufenpyrad, tolfenpyrad, and flufenerim; 
 an METI II and III compound selected from the group consisting of acequinocyl, fluacyprim, and hydramethyl-non; 
 chlorfenapyr; 
 an oxidative phosphorylation inhibitor selected from the group consisting of cyhexatin, diafenthiuron, fenbu-tatin oxide, and propargite; 
 cryomazine; 
 piperonyl butoxide; 
 a sodium channel blocker selected from the group consisting of indoxacarb and metaflumizone; and 
 another compound selected from the group consisting of benclothiaz, bifenazate, cartap, flonicamid, pyridalyl, pymetrozine, sulfur, thiocyclam, flubendiamide, chlorantraniliprole, cyazypyr (HGW86); cyenopyrafen, flupyrazofos, cyflumetofen, ami-doflumet, imicyafos, bistrifluoron, and pyrifluquinazon. 
 
   
     
     
         17 . The method as claimed in  claim 16 , wherein a commercially available formulation of the  Bacillus subtilis  strain is used. 
     
     
         18 . The method as claimed in  claim 16 , wherein component (II) is selected from the groups A) to G). 
     
     
         19 . The method as claimed in  claim 16 , wherein component (II) is selected from the groups A), B), C), E) and G). 
     
     
         20 . The method as claimed in  claim 16 , wherein component (II) is selected from group A). 
     
     
         21 . The method as claimed in  claim 16 , wherein component (II) is pyraclostrobin. 
     
     
         22 . The method as claimed in  claim 16 , wherein component (II) is selected from Bordeaux mixture, copper acetate, copper hydroxide, copper oxychloride, basic copper sulfate and sulfur. 
     
     
         23 . The method as claimed in  claim 16 , wherein
 a) the  Bacillus subtilis  strain with NRRL Accession No. B-21661 or a cell-free extract thereof, and/or a mutant of this strain or extract having all the identifying characteristics of the respective strain or extract as component (I) and   b) one chemical compound as component (II), selected from the active compound groups A) to J) as defined in  claim 16  are applied in a weight ratio of from 100:1 to 1:100.   
     
     
         24 . The method as claimed in  claim 16 , wherein the composition comprises
 a) the  Bacillus subtilis  strain with NRRL Accession No. B-21661 or a cell-free extract thereof, and/or a mutant of this strain or extract having all the identifying characteristics of the respective strain or extract as component (I) and   b) one chemical compound as component (II), selected from the active compound groups A) to J) as defined in  claim 16     are applied simultaneously, that is jointly or separately, or in succession.   
     
     
         25 . The method as claimed in  claim 16 , wherein the seed is treated. 
     
     
         26 . The method as claimed in  claim 16 , wherein the application is carried out as in-furrow and/or foliar treatment. 
     
     
         27 . The method as claimed in  claim 16 , wherein a repeated application is carried out. 
     
     
         28 . The method as claimed in  claim 16 , wherein component (II) selected from the active compound groups (A) to (J) is applied before the Pre-harvest interval while component (I) is applied during the Pre-harvest interval. 
     
     
         29 . The method as claimed in  claim 16 , wherein the agricultural plant is selected from soybean, corn, wheat, triticale, barley, oat, rye, rape, millet, rice, sunflower, cotton, sugar beet, pome fruit, stone fruit, citrus, banana, strawberry, blueberry, almond, grape, mango, papaya, peanut, potato, tomato, pepper, cucurbit, cucumber, melon, watermelon, garlic, onion, broccoli, carrot, cabbage, bean, dry bean, canola, pea, lentil, alfalfa, trefoil, clover, flax, elephant grass, grass, lettuce, sugarcane, tea, tobacco and coffee; each in its natural or genetically modified form.

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