Mammalian cell surface antigens; related reagents

Abstract

Purified genes encoding a T cell surface antigen from a mammal, reagents related thereto including purified proteins, specific antibodies, and nucleic acids encoding this antigen are provided. Methods of using said reagents and diagnostic kits are also provided.

1 - 22 . (canceled) 
     
     
         23 . A substantially pure polypeptide comprising an extracellular domain of SEQ ID NO: 2 or 4. 
     
     
         24 . The polypeptide of  claim 23 , wherein the polypeptide is conjugated to a chemical moiety. 
     
     
         25 . The polypeptide of  claim 23 , wherein the polypeptide is fused to a heterologous protein. 
     
     
         26 . The polypeptide of  claim 25 , wherein the heterologous protein is selected from the group consisting of luciferase, bacterial β-galactosidase, trpE, Protein A, β-lactamase, alpha amylase, alcohol dehydrogenase, yeast alpha mating factor, and His6 (SEQ ID NO:8). 
     
     
         27 . A pharmaceutical formulation comprising the polypeptide of  claim 23  and a pharmaceutically and physiologically acceptable carrier. 
     
     
         28 . A method of purifying the polypeptide of  claim 23  from other materials in a mixture comprising contacting the protein with an antibody that specifically binds the protein, and separating the bound protein from other materials. 
     
     
         29 . A substantially pure polypeptide comprising the amino acid sequence of SEQ ID NO: 2 or 4. 
     
     
         30 . The polypeptide of  claim 29  wherein the polypeptide is expressed on activated T cells. 
     
     
         31 . A pharmaceutical formulation comprising the polypeptide of  claim 29  and a pharmaceutically and physiologically acceptable carrier. 
     
     
         32 . A method of purifying the protein of  claim 29  from other materials in a mixture comprising contacting the protein with an antibody that specifically binds the protein, and separating the bound protein from other materials. 
     
     
         33 . The polypeptide of  claim 23 , wherein the polypeptide is expressed by T cells.