US2012096599A1PendingUtilityA1

Nucleic Acid Molecules and Other Molecules Associated with Plants and Uses Thereof for Plant Improvement

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Assignee: KOVALIC DAVID KPriority: May 8, 2000Filed: Dec 22, 2011Published: Apr 19, 2012
Est. expiryMay 8, 2020(expired)· nominal 20-yr term from priority
C07H 21/04C12N 15/8251C12N 15/8275C12N 15/8247C12N 15/8216C12N 15/8273C12N 15/82C12N 15/8274C07K 14/415C12N 15/8218C12N 15/8261
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Claims

Abstract

Recombinant polynucleotides and recombinant polypeptides useful for improvement of plants are provided. The disclosed recombinant polynucleotides and recombinant polypeptides find use in production of transgenic plants to produce plants having improved properties.

Claims

exact text as granted — not AI-modified
1 - 3 . (canceled) 
     
     
         4 . A transformed plant comprising a nucleic acid molecule which comprises:
 (a) an exogenous promoter region which functions in a plant cell to cause the production of an mRNA molecule; which is linked to;   (b) a structural nucleic acid molecule, wherein said structural nucleic acid molecule comprises a nucleic acid sequence, wherein said nucleic acid sequence shares between 100% and 90% sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 31,564, or the complement of SEQ ID NO: 1 through SEQ ID NO: 31,564,   
       which is operably linked to
 (c) a 3′ non-translated sequence that functions in said plant cell to cause the termination of transcription and the addition of polyadenylated ribonucleotides to said 3′ end of said mRNA molecule. 
 
     
     
         5 . The transformed plant according to  claim 4 , wherein said nucleic acid sequence is the complement of a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 31,564. 
     
     
         6 . The transformed plant according to  claim 4 , wherein said nucleic acid sequence is in the antisense orientation of a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 31,564. 
     
     
         7 . The transformed plant according to  claim 4 , wherein said nucleic acid sequence shares between 100% and 95% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 31,564 or the complement of SEQ ID NO: 1 through SEQ ID NO: 31,564. 
     
     
         8 . The transformed plant according to  claim 7 , wherein said nucleic acid sequence shares between 100% and 98% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 31,564 or the complement of SEQ ID NO: 1 through SEQ ID NO: 31,564. 
     
     
         9 . The transformed plant according to  claim 8 , wherein said nucleic acid sequence shares between 100% and 99% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 31,564 or the complement of SEQ ID NO: 1 through SEQ ID NO: 31,564. 
     
     
         10 . The transformed plant according to  claim 9 , wherein said nucleic acid sequence shares 100% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 31,564 and the complement of SEQ ID NO: 1 through SEQ ID NO: 31,564. 
     
     
         11 . A transformed seed comprising a transformed plant cell comprising a nucleic acid molecule which comprises:
 (a) an exogenous promoter region which functions in said plant cell to cause the production of an mRNA molecule; which is linked to;   (b) a structural nucleic acid molecule, wherein said structural nucleic acid molecule comprises a nucleic acid sequence, wherein said nucleic acid sequence shares between 100% and 90% sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 31,564, or the complement of SEQ ID NO: 1 through SEQ ID NO: 31,564,   
       which is linked to
 (c) a 3′ non-translated sequence that functions in said plant cell to cause the termination of transcription and the addition of polyadenylated ribonucleotides to said 3′ end of said mRNA molecule. 
 
     
     
         12 . The transformed seed according to  claim 11 , wherein said nucleic acid sequence is the complement of a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 31,564. 
     
     
         13 . The transformed seed according to  claim 11 , wherein said exogenous promoter region functions in a seed cell. 
     
     
         14 . The transformed seed according to  claim 11 , wherein said nucleic acid sequence shares between 100% and 95% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 31,564 or the complement of SEQ ID NO: 1 through SEQ ID NO: 31,564. 
     
     
         15 . The transformed seed according to  claim 14 , wherein said nucleic acid sequence shares between 100% and 98% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 31,564 or the complement of SEQ ID NO: 1 through SEQ ID NO: 31,564. 
     
     
         16 . The transformed seed according to  claim 15 , wherein said nucleic acid sequence shares between 100% and 99% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 31,564 or the complement of SEQ ID NO: 1 through SEQ ID NO: 31,564. 
     
     
         17 . The transformed seed according to  claim 16 , wherein said nucleic acid sequence shares 100% sequence identity with a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 31,564 and the complement of SEQ ID NO: 1 through SEQ ID NO: 31,564. 
     
     
         18 . A method of growing a transgenic plant comprising
 (a) planting a transformed seed comprising a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 31,564, or the complement of SEQ ID NO: 1 through SEQ ID NO: 31,564, and   (b) growing a plant from said seed.   
     
     
         19 . A substantially purified nucleic acid molecule comprising a nucleic acid sequence, wherein said nucleic acid sequence shares between 100% and 90% sequence identity to a nucleic acid sequence selected from the group consisting of SEQ ID NO: 1 through SEQ ID NO: 31,564, or the complement of SEQ ID NO: 1 through SEQ ID NO: 31,564. 
     
     
         20 . The substantially purified nucleic acid molecule of  claim 19 , wherein said nucleic acid molecule encodes a sorghum protein or fragment thereof.

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