Methods and materials for nanocrystalline surface coatings and attachment of peptide amphiphile nanofibers thereon

Abstract

Biocompatible composites comprising peptide amphiphiles and surface modified substrates and related methods for attachment thereon.

1 - 7 . (canceled) 
     
     
         8 . A method of promoting growth of an amine-modified calcium phosphate composition, said method comprising:
 providing a biocompatible substrate;   depositing a substantially single-phase calcium phosphate component on said substrate; and   introducing said substrate to a calcium phosphate medium, said medium comprising a poly(L-lysine) component.   
     
     
         9 . The method of claim  7  wherein said substrate contacts a medium comprising a reactive calcium reagent and a reactive phosphate reagent, said contact for a time sufficient to deposit said calcium phosphate component on said substrate. 
     
     
         10 . The method of  claim 8  wherein said calcium phosphate medium comprises at least one of a reactive calcium reagent and a reactive phosphate reagent. 
     
     
         11 . The method of  claim 10  wherein at least one of said reagents comprises said poly(L-lysine) component. 
     
     
         12 . The method of  claim 8  wherein said deposition comprises formation of crystalline calcium phosphate, and said introduction incorporates poly(L-lysine) into a calcium phosphate phase. 
     
     
         13 . The method of  claim 12  wherein said introduction induces a nanotextured component comprising calcium phosphate and poly(L-lysine). 
     
     
         14 . A method of coupling peptide amphiphiles to a biocompatible substrate, said method comprising:
 providing a biocompatible substrate;   depositing a substantially single-phase calcium phosphate component on said substrate;   depositing a mineral phase on said calcium phosphate phase, said mineral phase comprising calcium phosphate and poly(L-lysine) incorporated therein; and   contacting said poly(L-lysine) with peptide amphiphiles, at least one of said amphiphiles comprising a carboxy functionality.   
     
     
         15 . The method of  claim 14  wherein said peptide amphiphiles comprise a nanofiber assembly. 
     
     
         16 . The method of  claim 14  wherein said substrate contacts a medium comprising a reactive calcium reagent and a reactive phosphate reagent, said contact for a time sufficient to deposit said calcium phosphate component on said substrate. 
     
     
         17 . The method of  claim 14  wherein said mineral phase is the reaction product of a calcium reagent and a phosphate reagent, and introduction of poly(L-lysine) during said reaction. 
     
     
         18 . The method of  claim 14  further comprising contacting said mineral phase with at least one of an acid and a degradative enzyme. 
     
     
         19 . The method of  claim 14  wherein at least one of said peptide amphiphiles comprises an RGD sequence. 
     
     
         20 . The method of  claim 14  further comprising culturing mammalian cells on said peptide amphiphiles.