Umbilical cord biomaterial for medical use

Abstract

The present invention provides a biomaterial comprising a mammalian umbilical cord membrane. The biomaterial can additionally comprise Wharton's jelly and/or one or more umbilical cord vessels. The biomaterial is preferably dry, and can be flat, tubular, or shaped to fit a particular body structure. The invention further provides laminates comprising at least one layer of an umbilical cord membrane biomaterial. The invention further provides methods of making the biomaterial, and laminates comprising the biomaterial, and methods of using the biomaterial.

1 . An umbilical cord-derived biomaterial comprising an isolated mammalian umbilical cord membrane and Wharton's jelly, wherein said biomaterial comprises less than 20% water by weight. 
     
     
         2 . The biomaterial of  claim 1  that comprises at least one umbilical vessel. 
     
     
         3 . The biomaterial of  claim 1  that is decellularized. 
     
     
         4 . The biomaterial of  claim 1  comprising an exogenous bioactive molecule. 
     
     
         5 . The biomaterial of  claim 4 , wherein said bioactive molecule is a cytokine or a growth factor. 
     
     
         6 . The biomaterial of  claim 4 , wherein said bioactive molecule is an extracellular matrix protein. 
     
     
         7 . The biomaterial of  claim 6 , wherein said extracellular matrix protein is collagen, fibronectin, elastin, vitronectin, or hyaluronic acid. 
     
     
         8 . The biomaterial of  claim 4 , wherein said bioactive molecule is hyaluronic acid. 
     
     
         9 . The biomaterial of  claim 8 , wherein said hyaluronic acid is crosslinked to said umbilical cord membrane. 
     
     
         10 . The biomaterial of  claim 1 , comprising an exogenous polymer. 
     
     
         11 . The biomaterial of  claim 10 , wherein said exogenous polymer is a synthetic biodegradable polymer or an anionic polymer. 
     
     
         12 . The biomaterial of  claim 4 , wherein the bioactive molecule is an antibiotic, a hormone, a growth factor, an anti-tumor agent, an anti-fungal agent, an anti-viral agent, a pain medication, an anti-histamine, an anti-inflammatory agent, an anti-infective agent, a wound healing agent, a wound sealant, a cellular attractant, a scaffolding reagent, or a small molecule. 
     
     
         13 . The biomaterial of  claim 12 , further comprising a hydrogel composition. 
     
     
         14 . The biomaterial of  claim 1 , additionally comprising an exogenous stem cell. 
     
     
         15 . The biomaterial of  claim 3 , additionally comprising an exogenous stem cell. 
     
     
         16 . The biomaterial of  claim 14 , wherein said exogenous stem cell is a placental stem cell, a mesenchymal stem cell, an embryonic stem cell, or a somatic stem cell. 
     
     
         17 . The biomaterial of  claim 16 , wherein said somatic stem cell is a neural stem cell, a hepatic stem cell, a pancreatic stem cell, an endothelial stem cell, a cardiac stem cell, or a muscle stem cell. 
     
     
         18 . A laminate comprising a plurality of layers, wherein at least one of the layers comprises the biomaterial of  claim 1 . 
     
     
         19 . A method of producing a biomaterial comprising isolating and decellularizing a composition comprising an umbilical cord membrane. 
     
     
         20 . A biomaterial made by the method of  claim 19 . 
     
     
         21 . A method of delivering a therapeutic agent to a subject comprising contacting the subject with the composition of  claim 1 , wherein said composition comprises a therapeutic agent. 
     
     
         22 . A method of repairing a tympanic membrane having a deformity, comprising contacting said tympanic membrane with a biomaterial comprising an isolated mammalian umbilical cord membrane.