Polypeptides of strain Bacillus sp. P203

Abstract

A new strain Bacillus sp. P203 ( Bacillus plakortiensis ) is disclosed. Isolated mature functional polypeptide which is obtainable from the bacterium strain Bacillus sp. P203 deposited under accession number DSM 17419 are disclosed.

1 . Functional polypeptides encoded by polynucleotides comprised in the genome of strains of  Bacillus  species  Bacillus plankortiensis  deposited under accession number DSM 17419. 
     
     
         2 . An isolated mature functional polypeptide obtainable from the bacterium strain  Bacillus  sp. P203 deposited under accession number DSM 17419. 
     
     
         3 . The polypeptide of  claim 2  selected from the group consisting of:
 (a) a polypeptide comprising an amino acid sequence which has at least 70% identity with a sequence of a mature polypeptide comprised in the group of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10 and SEQ ID NO: 12; 
 (b) a polypeptide which is encoded a nucleotide sequence which hybridize under high stringency conditions with a polynucleotide probe selected from the group consisting of
 (i) the complementary strand to a nucleotide sequence selected from the group of regions of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9 and SEQ ID NO: 11 encoding a mature polypeptide. 
 (ii) the complementary strand to the cDNA sequence contained in a nucleotide sequences selected from the group of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9 and SEQ ID NO: 11 encoding a mature polypeptide. 
 
 (c) a fragment of a mature polypeptide comprised in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10 and SEQ ID NO: 12 
 wherein the polypeptide has a function of the corresponding mature polypeptides comprised in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10 and SEQ ID NO: 12. 
 
     
     
         4 . The polypeptide of  claim 2 , wherein the polypeptide is an enzyme having a function selected from the group consisting of xylanase, serine protease, carbonic anhydrase, rhamnogalacturonan lyase and galactanase. 
     
     
         5 . The enzyme of  claim 4  selected from the group consisting of:
 (a) an enzyme having an amino acid sequence which has at least 70% identity with an amino acid sequence selected from mature enzymes comprised in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10 and SEQ ID NO: 12; 
 (b) an enzyme which is encoded by a nucleotide sequence which hybridize under high stringency conditions with a polynucleotide probe selected from the group consisting of
 (i) the complementary strand to a nucleotide sequence selected from the group of regions of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9 and SEQ ID NO: 11 encoding the mature enzyme, 
 (ii) the complementary strand to the cDNA sequence contained in a nucleotide sequences selected from regions of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9 and SEQ ID NO: 11 encoding the mature polypeptide; 
 
 (c) a fragment of the mature enzyme comprised in SEQ SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10 and SEQ ID NO: 12, and 
 wherein the enzyme has a function of the corresponding mature polypeptides comprised in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10 and SEQ ID NO: 12. 
 
     
     
         6 . The polypeptide of  claim 2 , wherein the polynucleotide encoding the polypeptide consists of a nucleotide sequence selected from the group of regions of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9 and SEQ ID NO: 11 encoding a mature polypeptide or a sequence differing there from by virtue of the degeneracy of the genetic code. 
     
     
         7 . An isolated enzyme selected from the group consisting of:
 (a) an enzyme comprising an amino acid sequence which has at least 70% identity with the amino acid sequence of a mature enzyme selected from the group consisting of xylanase, serine protease, carbonic anhydrase, rhamnogalacturonan lyase and galactanase secreted from the strain of strain  Bacillus  sp. P203 deposited under DSM 17419;   (b) a polypeptide which is encoded by a nucleotide sequence which hybridize under high stringency conditions with a polynucleotide probe selected from the group consisting of:
 (i) the complementary strand to a nucleotide sequence comprised in the strain of strain  Bacillus  sp. P203 deposited under accession number DSM 17419 encoding a mature enzyme selected from the group consisting of xylanase, serine protease, carbonic anhydrase, rhamnogalacturonan lyase and galactanase secreted from that strain; 
 (ii) the complementary strand to the cDNA sequence contained in a nucleotide sequences comprised in the strain of strain  Bacillus  sp. P203 deposited under α-cession number DSM 17419 encoding a mature enzyme selected from the group consisting of xylanase, serine protease, carbonic anhydrase, rhamnogalacturonan lyase and galactanase secreted from that strain; 
   (c) a fragment of a mature enzyme selected from the group consisting of xylanase, serine protease, carbonic anhydrase, rhamnogalacturonan lyase and galactanase secreted from the strain of strain  Bacillus  sp. P203 deposited under accession number DSM 17419;   wherein the enzyme have a function selected from xylanase, serine protease, carbonic anhydrase, rhamnogalacturonan lyase and galactanase.   
     
     
         8 . A composition comprising the polypeptide of  claim 1 . 
     
     
         9 . The composition of  claim 8 , comprising at least two different polypeptides of  claims 1 . 
     
     
         10 . The composition of  claim 8 , comprising all polypeptides secreted when fermenting a sample of strain  Bacillus  sp. P203 DSM 17419 or a mutant thereof wherein one or more genes has been deleted or added. 
     
     
         11 . The composition of  claim 8  further comprising one or more additional enzymes. 
     
     
         12 . The composition of  claim 8 , characterized by being a detergent composition which, in addition to the polypeptide, comprises a surfactant. 
     
     
         13 . The composition of  claim 8 , characterized by being a feed composition which in addition to the polypeptide comprises a cereal or grain product. 
     
     
         14 . The composition of  claim 8 , characterized by being a food composition. 
     
     
         15 . The composition of  claim 8 , further comprising a polysaccharide or a mixture of polysaccharides. 
     
     
         16 . A method for preparing a composition of  claim 8 , comprising admixing the polypeptide of  claim 1  with an excipient. 
     
     
         17 . A polynucleotide having a nucleotide sequence which encodes for the polypeptide defined in  claim 1 . 
     
     
         18 . A composition comprising the polynucleotide of  claim 17 . 
     
     
         19 . A nucleic acid construct comprising the nucleotide sequence defined in  claim 17  operably linked to one or more control sequences that direct the production of the polypeptide in a host cell. 
     
     
         20 . A recombinant expression vector comprising the nucleic acid construct of  claim 19 . 
     
     
         21 . A recombinant host cell comprising the nucleic acid construct of  claim 19 . 
     
     
         22 . A method for producing the polypeptide of  claim 1  comprising:
 (a) cultivating a strain, which in its wild-type form is capable of producing the polypeptide, to produce the polypeptide; and 
 (b) recovering the polypeptide. 
 
     
     
         23 . A method for producing a polypeptide of  claim 1  comprising:
 (a) cultivating a recombinant host cell as defined in  claim 22  under conditions conducive for production of the polypeptide; and 
 (b) recovering the polypeptide. 
 
     
     
         24 . The method of  claim 23  comprising (i) fusing genes from the genome of the strain  Bacillus  sp. P203 deposited under accession number DSM 17419 with a gene encoding a signal-less reporter, via a transposon tag, (ii) growing host cell clones comprising a fused gene of the strain  Bacillus  sp. P203 (DSM 17419) in a medium revealing the presence of the reporter, (iii) detecting clones secreting the reporter and (iv) isolating gene and polypeptide of the strain  Bacillus  sp. P203 (DSM 17419) comprised in that clone. 
     
     
         25 . A storage medium suitable for use in an electronic device comprising information of the amino acid sequence of the polypeptide of  claim 1 . 
     
     
         26 . A process comprising employing a polypeptide of  claim 1  in an industrial or household technical process.