Lactobacillus Mutant

Abstract

The present invention discloses a Lactobacillus mutant, which is Lactobacillus paracasei subsp. paracasei PAN 101 and deposited in Agricultural Research Culture Collection (NRRL) in Nov. 13, 2009, and the accession number is NRRL B-50335; Lactobacillus paracasei subsp. paracasei PAN 101 can produce L(+)-lactate, and the production of L(+)-lactate in Lactobacillus paracasei subsp. paracasei PAN 101 can achieve a maximum amount by culturing Lactobacillus paracasei subsp. paracasei PAN 101 under an appropriate culture condition; Lactobacillus paracasei subsp. paracasei PAN 101 has the effect of acid resistance, alkali resistance and immune regulation.

1 . A  Lactobacillus  mutant, which is  Lactobacillus paracasei  subsp.  paracasei  PAN 101 and deposited in Agricultural Research Culture Collection (NRRL) in Nov. 13, 2009, wherein the accession number of  Lactobacillus paracasei  subsp.  paracasei  PAN 101 is NRRL B-50335. 
     
     
         2 . The  Lactobacillus  mutant according to  claim 1 , wherein  Lactobacillus paracasei  subsp.  paracasei  PAN 101 can produce L(+)-lactate. 
     
     
         3 . The  Lactobacillus  mutant according to  claim 1 , wherein  Lactobacillus paracasei  subsp.  paracasei  PAN 101 has the effect of acid resistance, alkali resistance and immune regulation. 
     
     
         4 . The  Lactobacillus  mutant according to  claim 1 , wherein the best culture temperature is 30° C. 
     
     
         5 . The  Lactobacillus  mutant according to  claim 1 , wherein the best culture pH value is 6.5. 
     
     
         6 . The  Lactobacillus  mutant according to  claim 1 , wherein the best culture pressure is 1 atm (1 atmosphere pressure). 
     
     
         7 . The  Lactobacillus  mutant according to  claim 1 , wherein a culture manner of  Lactobacillus paracasei  subsp.  paracasei  PAN 101 is selected from the group consisting of static culture and shaking culture. 
     
     
         8 . The  Lactobacillus  mutant according to  claim 1 , wherein a culture medium for culturing  Lactobacillus paracasei  subsp.  paracasei  PAN 101 is able to contain at least one carbon source, which is selected from the group consisting of: Glucose, Galactose, D-ribose, Xylose, Fructose, α-Lactose, Maltose, Sucrose, Trehalose, Raffinose, myo-Inositol, Sorbitol, D-mannitol, Citric acid, Dextrin, Starch, and Molasses. 
     
     
         9 . The  Lactobacillus  mutant according to  claim 1 , wherein a culture medium for culturing  Lactobacillus paracasei  subsp.  paracasei  PAN 101 is able to contain at least one nitrogen source, which is selected from the group consisting of: Yeast extract, Beef extract, Peptone, Soytone, Tryptose, Corn-steep liquor, Casein, Urea, Ammonium citrate, and Ammonium sulfate.