Soluble protein and utilization of the same

Abstract

Disclosed is a method for screening a medicine using a protein, and a compound obtained by the screening method. A compound or a salt thereof inhibiting activity or expression of the protein of the present invention, a neutralizing antibody against the protein, a polynucleotide that are complementary to a polynucleotide coding the protein, and the like can be used as an agent for preventing and/or treating neurodegenerative diseases and the like. Moreover, a compound or a salt thereof enhancing activity or expression of the protein of the present invention, the protein or a partial peptide thereof, a polynucleotide coding the protein, and the like can be used as an agent for preventing and/or treating cancers and the like. Furthermore, the protein of the present invention is useful as a reagent for screening a compound inhibiting or enhancing activity of the protein.

1 . An isolated protein consisting of the amino acid sequence of SEQ ID NO: 9 or 30, or a homologue thereof that is at least 95% homologous to said sequence. 
     
     
         2 . A polynucleotide comprising the nucleotide sequence encoding the isolated protein according to  claim 1 . 
     
     
         3 . The polynucleotide according to  claim 2 , comprising the nucleotide sequence of SEQ ID NO: 12 or 27. 
     
     
         4 . A recombinant vector comprising the polynucleotide according to  claim 2 . 
     
     
         5 . A transformant obtained by transforming a host cell with the recombinant vector according to  claim 4 . 
     
     
         6 . A method for producing a protein consisting of the amino acid sequence of SEQ ID NO: 9 or 30, or a homologue thereof that is at least 95% homologous to said sequence, comprising culturing the transformant according to  claim 5 , and thereby generating the protein. 
     
     
         7 . A recombinant vector comprising the polynucleotide according to  claim 3 . 
     
     
         8 . A transformant obtained by transforming a host cell with the recombinant vector according to  claim 7 . 
     
     
         9 . A method for producing a protein consisting of the amino acid sequence of SEQ ID NO: 9 or 30, or a homologue thereof that is at least 95% homologous to said sequence, comprising culturing the transformant according to  claim 8 , and thereby generating the protein.