Minicell based delivery of biologically active compounds

Abstract

Minicells are used to deliver biologically active compounds including radioisotopes, polypeptides, nucleic acids, small molecules, drug molecules, and chemotherapeutic agents. In some cases, the minicell displays ligands or binding moieties that target the minicell to a desired host cell.

1 . A fully intact eubacterial minicell derived from a eubacterial parent cell, wherein the minicell comprises a biologically active compound and displays an antibody or antibody derivative, wherein the biologically active compound is selected from the group consisting of a polypeptide, a small molecule, and a nucleic acid, and wherein the biologically active compound and the antibody or antibody derivative are exogenous to the parent cell and distinct from each other. 
     
     
         2 . The minicell of  claim 1 , wherein the biologically active compound is a polypeptide. 
     
     
         3 . The minicell of  claim 2 , wherein the polypeptide is a fusion protein. 
     
     
         4 . The minicell of  claim 2 , wherein the polypeptide is a soluble polypeptide. 
     
     
         5 . The minicell of  claim 2 , wherein the polypeptide is a protein toxin. 
     
     
         6 . The minicell of  claim 1 , wherein the biologically active compound is a small molecule. 
     
     
         7 . The minicell of  claim 6 , wherein the small molecule is a drug molecule. 
     
     
         8 . The minicell of  claim 1 , wherein the biologically active compound is a nucleic acid. 
     
     
         9 . The minicell of  claim 8 , wherein the nucleic acid comprises an antisense nucleic acid. 
     
     
         10 . The minicell of  claim 8 , wherein the nucleic acid comprises a ribozyme. 
     
     
         11 . The minicell of  claim 8 , wherein the nucleic acid comprises an aptamer. 
     
     
         12 . The minicell of  claim 1 , wherein the minicell displays an antibody. 
     
     
         13 . The minicell of  claim 1 , wherein the minicell displays an antibody derivative. 
     
     
         14 . A fully intact eubacterial minicell derived from a eubacterial parent cell, wherein the minicell comprises a biologically active compound and displays an antibody or antibody derivative, wherein the biologically active compound is a therapeutic polypeptide, and wherein the biologically active compound and the antibody or antibody derivative are exogenous to the parent cell and distinct from each other. 
     
     
         15 . The minicell of  claim 14 , wherein the therapeutic polypeptide is a soluble polypeptide. 
     
     
         16 . The minicell of  claim 14 , wherein the therapeutic polypeptide is a protein toxin. 
     
     
         17 . A fully intact eubacterial minicell derived from a eubacterial parent cell, wherein the minicell comprises a biologically active compound and displays an antibody or antibody derivative, wherein the biologically active compound is a therapeutic nucleic acid, and wherein the biologically active compound and the antibody or antibody derivative are exogenous to the parent cell and distinct from each other. 
     
     
         18 . The minicell of  claim 17 , wherein the therapeutic nucleic acid comprises an antisense nucleic acid. 
     
     
         19 . The minicell of  claim 17 , wherein the therapeutic nucleic acid comprises a ribozyme. 
     
     
         20 . The minicell of  claim 17 , wherein the therapeutic nucleic acid comprises an aptamer.