Soybean promoter ltp4 and flower-preferred expression thereof in transgenic plants

Abstract

The promoter of a soybean lipid transfer protein LTP4 and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants are described.

1 - 19 . (canceled) 
     
     
         20 . An isolated polynucleotide comprising:
 (a) a nucleotide sequence encoding a polypeptide having lipid protein transferase activity, wherein the polypeptide has at least 80% sequence identity, based on the Clustal method of alignment, when compared to the sequence set forth in SEQ ID NO:15, or   (b) a full-length complement of the nucleotide sequence of (a).   
     
     
         21 . The isolated polynucleotide of  claim 20 , wherein the polypeptide has at least 85% sequence identity, based on the Clustal method of alignment, when compared to the sequence set forth in SEQ ID NO:15. 
     
     
         22 . The isolated polynucleotide of  claim 20 , wherein the polypeptide has at least 90% sequence identity, based on the Clustal method of alignment, when compared to the sequence set forth in SEQ ID NO:15. 
     
     
         23 . The isolated polynucleotide of  claim 20 , wherein the polypeptide has at least 95% sequence identity, based on the Clustal method of alignment, when compared to the sequence set forth in SEQ ID NO:15. 
     
     
         24 . The isolated polynucleotide of  claim 20  encoding the sequence set forth in SEQ ID NO:15. 
     
     
         25 . The isolated polynucleotide of  claim 20 , wherein the nucleotide sequence comprises the sequence set forth in SEQ ID NO:14. 
     
     
         26 . A vector comprising the isolated polynucleotide of  claim 20 . 
     
     
         27 . A recombinant DNA construct comprising the isolated polynucleotide of  claim 20  operably linked to a regulatory sequence. 
     
     
         28 . A cell comprising the recombinant DNA construct of  claim 27 . 
     
     
         29 . A plant comprising the recombinant DNA construct of  claim 27 . 
     
     
         30 . A seed comprising the recombinant DNA construct of  claim 27 . 
     
     
         31 . A method for transforming a cell, comprising transforming a cell with the isolated polynucleotide of  claim 20 . 
     
     
         32 . A method for producing a plant comprising transforming a plant cell with the isolated polynucleotide of  claim 20  and regenerating a plant from the transformed plant cell. 
     
     
         33 . An isolated polypeptide having lipid protein transferase activity, wherein the isolated polypeptide has at least 80% sequence identity, based on the Clustal method of alignment, when compared to the sequence set forth in SEQ ID NO:15. 
     
     
         34 . The isolated polypeptide of  claim 33 , wherein the isolated polypeptide has at least 85% sequence identity, based on the Clustal method of alignment, when compared to the sequence set forth in SEQ ID NO:15. 
     
     
         35 . The isolated polypeptide of  claim 33 , wherein the isolated polypeptide has at least 90% sequence identity, based on the Clustal method of alignment, when compared to the sequence set forth in SEQ ID NO:15. 
     
     
         36 . The isolated polypeptide of  claim 33 , wherein the isolated polypeptide has at least 95% sequence identity, based on the Clustal method of alignment, when compared to the sequence set forth in SEQ ID NO:15. 
     
     
         37 . The isolated polypeptide of  claim 33 , wherein the isolated polypeptide comprises the amino acid sequence set forth in SEQ ID NO:15.