Method for quantitatively determining ldl cholesterols

Abstract

A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.

1 . A method for quantitatively determining low density lipoprotein cholesterol, comprising the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent, to thereby induce preferential reactions of cholesterols in high density- and very low density-lipoproteins among lipoproteins, and subsequently determining the amount of cholesterol which reacts thereafter. 
     
     
         2 . A method for quantitatively determining low density lipoprotein cholesterol, characterized by comprising the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers, a substance exhibiting stronger bonding affinity to very low density lipoprotein than to low density lipoprotein, and a cholesterol-assaying enzyme reagent, to thereby induce preferential reactions cholesterols in high density- and very low density-lipoproteins among lipoproteins, and subsequently, determining the amount of cholesterol which reacts thereafter. 
     
     
         3 . The method for quantitatively determining low “density lipoprotein cholesterol according to  claim 2 , wherein the substance exhibiting stronger bonding affinity to very ‘low density lipoprotein than to low density lipoprotein is a’polyanion or a substance forming a divalent metal salt. 
     
     
         4 . A kit for quantitatively determining low density lipoprotein cholesterol, comprising a cholesterol-assaying enzyme reagent and a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers. 
     
     
         5 . The kit for quantitative determination according to  claim 4 , further comprising a substance which exhibits stronger bonding affinity to very low density lipoprotein than to low density lipoprotein.