Enhanced biological autologous tissue adhesive composition and methods of preparation and use

Abstract

The invention includes a composition useful for preparing a tissue sealant for use on a patient, comprising: autologous fibrinogen, an activating agent and at least one supplement. The invention includes a method of treating a disc having at least one defect, comprising: introducing a composition into the disc, wherein the composition comprises autologous fibrinogen and an activating agent, and wherein the composition forms fibrin.

1 . A composition useful for preparing a tissue sealant for use on a patient, comprising: autologous fibrinogen, an activating agent and at least one supplement. 
     
     
         2 . The composition of  claim 1 , wherein the activating agent is derived from a snake venom. 
     
     
         3 . The composition of  claim 1 , wherein the at least one supplement is selected from the group consisting of analgesics, anesthetics, antimicrobial compounds, antibiotics, antifibrinolytic agents, anti-inflammatory compounds, antibodies, anticoagulants, antifungal compounds, antiangiogenins, antiseptics, cardiovascular drugs, cytokines, cytotoxins or cell proliferation inhibiting compounds, chemotherapeutic drugs, interferons, growth factors, hormones, lipids or liposomes, oligonucleotides or polynucleotides, osteoinducers, cartilage-inducing compounds, polymers, polysaccharides, proteoglycans, polypeptides, protease inhibitors, steroids, vasoconstrictors, vasodilators, vitamins, nutritional supplements, minerals, stabilizers and combinations thereof. 
     
     
         4 . A tissue sealant formed by reaction of the composition of  claim 1 . 
     
     
         5 . The composition of  claim 1 , further comprising a contrast medium. 
     
     
         6 . The composition of  claim 1 , wherein at least one supplement is an anesthetic. 
     
     
         7 . A method of treating tissue of a patient wherein the tissue has at least one defect, comprising: applying a composition to the tissue, wherein the composition comprises autologous fibrinogen and an activating agent, and wherein the composition forms fibrin. 
     
     
         8 . The method of  claim 7 , wherein the activating agent is derived from snake venom. 
     
     
         9 . The method of  claim 7 , wherein the composition includes at least one supplement, wherein the at least one supplement is selected from the group consisting of analgesics, anesthetics, antimicrobial compounds, antibiotics, antifibrinolytic agents, anti-inflammatory compounds, antibodies, anticoagulants, antifungal compounds, antiangiogenins, antiseptics, cardiovascular drugs, cytokines, cytotoxins or cell proliferation inhibiting compounds, chemotherapeutic drugs, interferons, growth factors, hormones, lipids or liposomes, oligonucleotides or polynucleotides, osteoinducers, cartilage-inducing compounds, polymers, polysaccharides, proteoglycans, polypeptides, protease inhibitors, steroids, vasoconstrictors, vasodilators, vitamins, nutritional supplements, minerals, stabilizers and combinations thereof. 
     
     
         10 . The composition of  claim 7 , wherein the composition includes an anesthetic. 
     
     
         11 . The method of  claim 7 , wherein the composition is applied by injecting the composition into the tissue. 
     
     
         12 . The method of  claim 7 , wherein the composition is applied by applying a coating on the surface of the tissue. 
     
     
         13 . A method of treating a human spinal disc having at least one defect, comprising: introducing a composition into the disc, wherein the composition comprises autologous fibrinogen and an activating agent, and wherein the composition forms fibrin. 
     
     
         14 . The method of  claim 13 , wherein the activating agent is derived from snake venom. 
     
     
         15 . The method of  claim 13 , wherein the composition includes at least one supplement, wherein the at least one supplement is selected from the group consisting of analgesics, anesthetics, antimicrobial compounds, antibiotics, antifibrinolytic agents, anti-inflammatory compounds, antibodies, anticoagulants, antifungal compounds, antiangiogenins, antiseptics, cardiovascular drugs, cytokines, cytotoxins or cell proliferation inhibiting compounds, chemotherapeutic drugs, interferons, growth factors, hormones, lipids or liposomes, oligonucleotides or polynucleotides, osteoinducers, cartilage-inducing compounds, polymers, polysaccharides, proteoglycans, polypeptides, protease inhibitors, steroids, vasoconstrictors, vasodilators, vitamins, nutritional supplements, minerals, stabilizers and combinations thereof. 
     
     
         16 . The method of  claim 13 , wherein the composition includes an anesthetic. 
     
     
         17 . The method of  claim 13 , wherein the composition is introduced by injecting the composition into the disc. 
     
     
         18 . The method of  claim 13 , wherein the at least one defect is a defect of the annulus fibrosus. 
     
     
         19 . The method of  claim 13 , wherein the composition includes an anesthetic.