Tumor Suppressor Gene

Abstract

A full-length cDNA encoding novel proteins involved in the control of cell proliferation (human Gros1-L and S) was successfully isolated from the human testis cDNA libraries. A full-length cDNA encoding the mouse homologues of the human Gros1 (mouse Gros1-L and S) was also isolated. The colony forming activity of cells exogenously expressing Gros1-L was significantly reduced, while that of cells expressing Gros1 antisense RNA was significantly increased.

1 . An isolated nucleic acid comprising a sequence encoding a protein comprising the amino acid sequence of SEQ ID NO:2. 
     
     
         2 . A vector into which the nucleic acid of  claim 1  is inserted. 
     
     
         3 . An isolated transformant harboring the nucleic acid of  claim 1 . 
     
     
         4 . An isolated transformant harboring the vector of  claim 2 . 
     
     
         5 . A method for producing a polypeptide, the method comprising the steps of culturing the transformant of  claim 3  and recovering the protein from the transformant or the culture supernatant thereof. 
     
     
         6 . The nucleic acid of  claim 1 , wherein the nucleic acid comprises the coding region of the nucleotide sequence of SEQ ID NO:1. 
     
     
         7 . The nucleic acid of  claim 1 , wherein the protein consists of the amino acid sequence of SEQ ID NO:2. 
     
     
         8 . An isolated nucleic acid comprising a sequence encoding a protein that comprises the amino acid sequence of SEQ ID NO:2 in which ten or fewer amino acids are substituted, deleted, and/or inserted. 
     
     
         9 . A vector into which the nucleic acid of  claim 8  is inserted. 
     
     
         10 . An isolated transformant harboring the nucleic acid of  claim 8 . 
     
     
         11 . An isolated transformant harboring the vector of  claim 9 . 
     
     
         12 . A method for producing a polypeptide, the method comprising the steps of culturing the transformant of  claim 10  and recovering the protein from the transformant or the culture supernatant thereof. 
     
     
         13 . The nucleic acid of  claim 8 , wherein the protein comprises the amino acid sequence of SEQ ID NO:2 in which ten or fewer amino acids are conservatively substituted. 
     
     
         14 . An isolated polynucleotide that (a) hybridizes with a nucleic acid consisting of the nucleotide sequence of SEQ ID NO:1 or the complementary strand thereof and (b) comprises at least 15 nucleotides. 
     
     
         15 . An isolated nucleic acid that hybridizes under highly stringent conditions with a polynucleotide probe consisting of the complement of nucleotides 52 to 1140 of SEQ ID NO:1, wherein said highly stringent conditions comprise washing in 2×SSC, 0.01% SDS three times at room temperature for 20 minutes, followed by washing in 1×SSC, 0.1% SDS three times at 37° C. for 20 minutes, and then washing in 1×SSC, 0.1% SDS twice at 50° C. for 20 minutes. 
     
     
         16 . A vector into which the nucleic acid of  claim 15  is inserted. 
     
     
         17 . A transformant harboring the nucleic acid of  claim 15 . 
     
     
         18 . A transformant harboring the vector of  claim 16 . 
     
     
         19 . A method for producing a polypeptide, the method comprising the steps of culturing the transformant of  claim 17  and recovering a protein encoded by the nucleic acid from the transformant or the culture supernatant thereof.