US2012171189A1PendingUtilityA1

Polypeptides of Botryosphaeria Rhodina

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Assignee: SCHNORR KIRK MATTHEWPriority: Aug 6, 2004Filed: Feb 8, 2012Published: Jul 5, 2012
Est. expiryAug 6, 2024(expired)· nominal 20-yr term from priority
Inventors:Kirk Matthew SchnorrLene LangePernille Uldall Bolvig
C12Y 302/01032C12N 9/58C12Y 302/01099C12Y 302/01021C12N 9/248C12Y 302/01008C07K 14/37C12N 9/0046A61P 43/00C12N 9/18C12N 9/2402
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Claims

Abstract

The invention relates to functional polypeptides secreted from Botryospaeria rhodina CBS 274.96.

Claims

exact text as granted — not AI-modified
1 . An isolated polypeptide selected from the group consisting of:
 (a) a polypeptide comprising an amino acid sequence which has at least 90% identity with a sequence of a mature polypeptide comprised in the group consisting of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO: 30, SEQ ID NO: 32, SEQ ID NO: 34, SEQ ID NO:36, SEQ ID NO: 38, SEQ ID NO: 40 and SEQ ID NO: 42; and   (b) a polypeptide which is encoded by a nucleotide sequence which hybridize under high stringency conditions with a polynucleotide probe selected from the group consisting of
 (i) the complementary strand to a nucleotide sequence selected from the group of regions of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO:23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39 and SEQ ID NO: 41 encoding a mature polypeptide, 
 (ii) the complementary strand to the cDNA sequence contained in a nucleotide sequences selected from the group of regions of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO:23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31, SEQ ID NO: 33, SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39 and SEQ ID NO: 41 encoding a mature polypeptide, 
   
       wherein the polypeptide has a function of the corresponding mature polypeptides comprised in the group consisting of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO: 30, SEQ ID NO: 32, SEQ ID NO: 34, SEQ ID NO:36, SEQ ID NO: 38, SEQ ID NO: 40 and SEQ ID NO: 42. 
     
     
         2 . An isolated enzyme selected from the group consisting of:
 (a) an enzyme comprising an amino acid sequence which has at least 90% identity with the amino acid sequence of a mature enzyme selected from the group consisting of xylanase, serine esterase, peroxidase, GH 61A polypeptide, GH 61B polypeptide, GH 61C polypeptide, GH 61D polypeptide, beta-glucosidase, endo-arabinase and pepsin peptidase secreted from the strain of  Botryosphaeria rhodina  deposited under CBS accession No. 274.96,   (b) a polypeptide which is encoded by a nucleotide sequence which hybridizes under high stringency conditions with a polynucleotide probe selected from the group consisting of
 (i) the complementary strand to a nucleotide sequence comprised in the strain of  Botryosphaeria rhodina  deposited under CBS accession No. 274.96 encoding a mature enzyme selected from the group consisting of xylanase, serine esterase, peroxidase, GH 61A polypeptide, GH 61B polypeptide, GH 61C polypeptide, GH 61D polypeptide, beta-glucosidase, endo-arabinase and pepsin peptidase secreted from that strain; 
 ii) the complementary strand to the cDNA sequence contained in a nucleotide sequences comprised in the strain of  Botryosphaeria rhodina  deposited under CBS accession No. 274.96 encoding a mature enzyme selected from the group consisting of xylanase, serine esterase, peroxidase, GH 61A polypeptide, GH 61B polypeptide, GH 61C polypeptide, GH 61D polypeptide, beta-glucosidase, endo-arabinase and pepsin peptidase secreted from that strain; 
   
       wherein the enzyme has a function selected from xylanase, serine esterase, peroxidase, GH 61A polypeptide, GH 61B polypeptide, GH 61C polypeptide, GH 61D polypeptide, beta-glucosidase, endo-arabinase and pepsin peptidase. 
     
     
         3 . The polypeptide of  claim 1  or  2  selected from the group consisting of:
 a) a polypeptide comprising an amino acid sequence which has at least 90% identity with a sequence selected from the group consisting of amino acids 1-291 of SEQ ID NO:2; 1-202 of SEQ ID NO:4; amino acids 1-352 of SEQ ID NO:6; amino acids 1-431 of SEQ ID NO:8; amino acids 1-185 of SEQ ID NO:10; amino acids 1-218 of SEQ ID NO:12; amino acids 1-249 of SEQ ID NO:14; amino acids 1-255 of SEQ ID NO:16; amino acids 1-205 of SEQ ID NO:18; amino acids 1-243 of SEQ ID NO:20; amino acids 1-415 of SEQ ID NO:22; amino acids 1-377 of SEQ ID NO:24; amino acids 1-259 of SEQ ID NO:26; amino acids 1-248 of SEQ ID NO:28; amino acids 1-149 of SEQ ID NO:30, amino acids 1-202 of SEQ ID NO:32; amino acids 1 to 603 of SEQ ID NO: 34; amino acids 1 to 301 of SEQ ID NO: 36; amino acids 1 to 438 of SEQ ID NO: 38; amino acids 1 to 396 of SEQ ID NO: 40 and amino acids 1 to 262 of SEQ ID NO: 42; 
 b) a polypeptide which is encoded by a nucleotide sequence which hybridizes under high stringency conditions with a polynucleotide selected from the group consisting of:
 i) the complementary strand of a nucleotide sequence selected from the group consisting of nucleotides 55-927 of SEQ ID NO:1; nucleotides 58-663 of SEQ ID NO:3; nucleotides 55-1110 of SEQ ID NO:5; nucleotides 55-1347 of SEQ ID NO:7; nucleotides 1-555 of SEQ ID NO:9; nucleotides 49-702 of SEQ ID NO:11; nucleotides 40-786 of SEQ ID NO:13; nucleotides 55-819 of SEQ ID NO:15; nucleotides 61-675 of SEQ ID NO:17; nucleotides 1-729 of SEQ ID NO:19; nucleotides 55-1299 of SEQ ID NO:21; nucleotides 49-1179 of SEQ ID NO:23; nucleotides 70-846 of SEQ ID NO:25; nucleotides 58-810 of SEQ ID NO:27; nucleotides 55-660 of SEQ ID NO:31; nucleotides 46 to 1854 of SEQ ID NO: 33; nucleotides 64 to 966 of SEQ ID NO: 35; nucleotides 55 to 1368 of SEQ ID NO: 37; nucleotides 61 to 1248 of SEQ ID NO: 39 and nucleotides 64 to 849 of SEQ ID NO: 41; 
 ii) the complementary strand to the cDNA sequence contained in a nucleotide sequences selected from the group of regions consisting of nucleotides 55-927 of SEQ ID NO:1; nucleotides 58-663 of SEQ ID NO:3; nucleotides 55-1110 of SEQ ID NO:5; nucleotides 55-1347 of SEQ ID NO:7; nucleotides 1-555 of SEQ ID NO:9; nucleotides 49-702 of SEQ ID NO:11; nucleotides 40-786 of SEQ ID NO:13; nucleotides 55-819 of SEQ ID NO:15; nucleotides 61-675 of SEQ ID NO:17; nucleotides 1-729 of SEQ ID NO:19; nucleotides 55-1299 of SEQ ID NO:21; nucleotides 49-1179 of SEQ ID NO:23; nucleotides 70-846 of SEQ ID NO:25; nucleotides 58-810 of SEQ ID NO:27; nucleotides 55-660 of SEQ ID NO:31; nucleotides 46 to 1854 of SEQ ID NO: 33; nucleotides 64 to 966 of SEQ ID NO: 35; nucleotides 55 to 1368 of SEQ ID NO: 37; nucleotides 61 to 1248 of SEQ ID NO: 39 and nucleotides 64 to 849 of SEQ ID NO: 41. 
 
 
     
     
         4 . The polypeptide of  claim 3 , wherein the polypeptide is an enzyme selected from the group consisting of a polypeptide having an amino acid sequence which has at least 95% identity with an amino acid sequence selected from the group consisting of amino acids 1-291 of SEQ ID NO:2; 1-202 of SEQ ID NO:4; amino acids 1-352 of SEQ ID NO:6; amino acids 1-431 of SEQ ID NO:8; amino acids 1-185 of SEQ ID NO:10; amino acids 1-218 of SEQ ID NO:12; amino acids 1-249 of SEQ ID NO:14; amino acids 1-255 of SEQ ID NO:16; amino acids 1-205 of SEQ ID NO:18; amino acids 1-243 of SEQ ID NO:20; amino acids 1-415 of SEQ ID NO:22; amino acids 1-377 of SEQ ID NO:24; amino acids 1-259 of SEQ ID NO:26; amino acids 1-248 of SEQ ID NO:28; amino acids 1-149 of SEQ ID NO:30; amino acids 1-202 of SEQ ID NO:32; amino acids 1 to 603 of SEQ ID NO: 34; amino acids 1 to 301 of SEQ ID NO: 36; amino acids 1 to 438 of SEQ ID NO: 38; amino acids 1 to 396 of SEQ ID NO: 40 and amino acids 1 to 262 of SEQ ID NO: 42. 
     
     
         5 . The polypeptide of  claim 4 , wherein the polypeptide is an enzyme selected from the group consisting of a polypeptide which is encoded by a nucleotide sequence which hybridize under very high stringency conditions with a polynucleotide selected from the group consisting of
 i) the complementary strand to a nucleotide sequence selected from the group of regions consisting of nucleotides 55-927 of SEQ ID NO:1; nucleotides 58-663 of SEQ ID NO:3; nucleotides 55-1110 of SEQ ID NO:5; nucleotides 55-1347 of SEQ ID NO:7; nucleotides 1-555 of SEQ ID NO:9; nucleotides 49-702 of SEQ ID NO:11; nucleotides 40-786 of SEQ ID NO:13; nucleotides 55-819 of SEQ ID NO:15; nucleotides 61-675 of SEQ ID NO:17; nucleotides 1-729 of SEQ ID NO:19; nucleotides 55-1299 of SEQ ID NO:21; nucleotides 49-1179 of SEQ ID NO:23; nucleotides 70-846 of SEQ ID NO:25; nucleotides 58-810 of SEQ ID NO:27; nucleotides 55-660 of SEQ ID NO:31; nucleotides 46 to 1854 of SEQ ID NO: 33; nucleotides 64 to 966 of SEQ ID NO: 35; nucleotides 55 to 1368 of SEQ ID NO: 37; nucleotides 61 to 1248 of SEQ ID NO: 39 and nucleotides 64 to 849 of SEQ ID NO: 41 and   ii) the complementary strand to the cDNA sequence contained in a nucleotide sequences selected from the group of regions consisting of nucleotides 55-927 of SEQ ID NO:1; nucleotides 58-663 of SEQ ID NO:3; nucleotides 55-1110 of SEQ ID NO:5; nucleotides 55-1347 of SEQ ID NO:7; nucleotides 1-555 of SEQ ID NO:9; nucleotides 49-702 of SEQ ID NO:11; nucleotides 40-786 of SEQ ID NO:13; nucleotides 55-819 of SEQ ID NO:15; nucleotides 61-675 of SEQ ID NO:17; nucleotides 1-729 of SEQ ID NO:19; nucleotides 55-1299 of SEQ ID NO:21; nucleotides 49-1179 of SEQ ID NO:23; nucleotides 70-846 of SEQ ID NO:25; nucleotides 58-810 of SEQ ID NO:27; nucleotides 55-660 of SEQ ID NO:31 nucleotides 46 to 1854 of SEQ ID NO: 33; nucleotides 64 to 966 of SEQ ID NO: 35; nucleotides 55 to 1368 of SEQ ID NO: 37; nucleotides 61 to 1248 of SEQ ID NO: 39 and nucleotides 64 to 849 of SEQ ID NO: 41.   
     
     
         6 . The polypeptide of  claim 3 , wherein the polynucleotide encoding the polypeptide consists of a polypeptide selected from the group consisting of amino acids 1-291 of SEQ ID NO:2; 1-202 of SEQ ID NO:4; amino acids 1-352 of SEQ ID NO:6; amino acids 1-431 of SEQ ID NO:8; amino acids 1-185 of SEQ ID NO:10; amino acids 1-218 of SEQ ID NO:12; amino acids 1-249 of SEQ ID NO:14; amino acids 1-255 of SEQ ID NO:16; amino acids 1-205 of SEQ ID NO:18; amino acids 1-243 of SEQ ID NO:20; amino acids 1-415 of SEQ ID NO:22; amino acids 1-377 of SEQ ID NO:24; amino acids 1-259 of SEQ ID NO:26; amino acids 1-248 of SEQ ID NO:28; amino acids 1-149 of SEQ ID NO:30; amino acids 1-202 of SEQ ID NO:32; amino acids 1 to 603 of SEQ ID NO: 34; amino acids 1 to 301 of SEQ ID NO: 36; amino acids 1 to 438 of SEQ ID NO: 38; amino acids 1 to 396 of SEQ ID NO: 40 and amino acids 1 to 262 of SEQ ID NO: 42. 
     
     
         7 . The polypeptide of  claim 3 , wherein the polypeptide is a GH10 xylanase comprising an amino acid sequence which has at least 90% identity with a GH10 xylanase obtainable from  Botryosphaeria rhodina  deposited under deposit accession number CBS 274.96. 
     
     
         8 . The polypeptide of  claim 3 , wherein the polypeptide is a GH10 xylanase comprising amino acids 1-291 of SEQ ID NO:2. 
     
     
         9 . The polypeptide of  claim 3 , wherein the polypeptide is a GH10 xylanase consisting of amino acids 1-291 of SEQ ID NO:2. 
     
     
         10 . The polypeptide of  claim 3 , wherein the polypeptide is a GH11 xylanase comprising an amino acid sequence which has at least 90% identity with a GH11 xylanase obtainable from  Botryosphaeria rhodina  deposited under deposit accession number CBS 274.96. 
     
     
         11 . The polypeptide of  claim 3 , wherein the polypeptides is a GH11 xylanase comprising amino acids 1-202 of SEQ ID NO:4. 
     
     
         12 . The polypeptide of  claim 3 , wherein the polypeptides is a GH11 xylanase consisting of amino acids 1-202 of SEQ ID NO:4. 
     
     
         13 . The polypeptide of  claim 3 , wherein the polypeptide is a serine esterase comprising an amino acid sequence which has at least 90% identity with a serine esterase obtainable from  Botryosphaeria rhodina  deposited under deposit accession number CBS 274.96. 
     
     
         14 . The polypeptide of  claim 3 , wherein the polypeptide is a serine esterase comprising amino acids 1-352 of SEQ ID NO:6. 
     
     
         15 . The polypeptide of  claim 3 , wherein the polypeptide is a serine esterase consisting of amino acids 1-352 of SEQ ID NO:6. 
     
     
         16 . The polypeptide of  claim 3 , wherein the polypeptide is a lipase comprising an amino acid sequence which has at least 90% identity with a serine esterase obtainable from  Botryosphaeria rhodina  deposited under deposit accession number CBS 274.96. 
     
     
         17 . The polypeptide of  claim 3 , wherein the polypeptide is a lipase comprising amino acids 1-431 of SEQ ID NO:8. 
     
     
         18 . The polypeptide of  claim 3 , wherein the polypeptide is a lipase consisting of amino acids 1-431 of SEQ ID NO:8. 
     
     
         19 . The polypeptide of  claim 3 , wherein the polypeptide is a peroxidase comprising an amino acid sequence which has at least 90% identity with a peroxidase obtainable from  Botryosphaeria rhodina  deposited under deposit accession number CBS 274.96. 
     
     
         20 . The polypeptide of  claim 3 , wherein the polypeptide is a peroxidase comprising amino acids 1-185 of SEQ ID NO:10. 
     
     
         21 . The polypeptide of  claim 3 , wherein the polypeptide is a peroxidase consisting of amino acids 1-185 of SEQ ID NO:10. 
     
     
         22 . The polypeptide of  claim 3 , wherein the polypeptide is a GH 61A polypeptide comprising an amino acid sequence which has at least 90% identity with a GH 61A polypeptide obtainable from  Botryosphaeria rhodina  deposited under deposit accession number CBS 274.96. 
     
     
         23 . The polypeptide of  claim 3 , wherein the polypeptide is a GH 61A polypeptide comprising amino acids 1-218 of SEQ ID NO:12. 
     
     
         24 . The polypeptide of  claim 3 , wherein the polypeptide is a GH 61A polypeptide consisting of amino acids 1-218 of SEQ ID NO:12. 
     
     
         25 . The polypeptide of  claim 3 , wherein the polypeptide is a GH 61B polypeptide comprising an amino acid sequence which has at least 90% identity with a GH 61B polypeptide obtainable from  Botryosphaeria rhodina  deposited under deposit accession number CBS 274.96. 
     
     
         26 . The polypeptide of  claim 3 , wherein the polypeptide is a GH 61B polypeptide comprising amino acids 1-249 of SEQ ID NO:14. 
     
     
         27 . The polypeptide of  claim 3 , wherein the polypeptide is a GH 61B polypeptide consisting of amino acids 1-249 of SEQ ID NO:14. 
     
     
         28 . The polypeptide of  claim 3 , wherein the polypeptide is a GH 61C polypeptide comprising an amino acid sequence which has at least 90% identity with a GH 61C polypeptide obtainable from  Botryosphaeria rhodina  deposited under deposit accession number CBS 274.96. 
     
     
         29 . The polypeptide of  claim 3 , wherein the polypeptide is a GH 61C polypeptide comprising amino acids 1-255 of SEQ ID NO:16. 
     
     
         30 . The polypeptide of  claim 3 , wherein the polypeptide is a GH 61C polypeptide consisting of amino acids 1-255 of SEQ ID NO:16. 
     
     
         31 . The polypeptide of  claim 3 , wherein the polypeptide is a GH 61D polypeptide comprising an amino acid sequence which has at least 90% identity with a GH 61D polypeptide obtainable from  Botryosphaeria rhodina  deposited under deposit accession number CBS 274.96. 
     
     
         32 . The polypeptide of  claim 3 , wherein the polypeptide is a GH 61D polypeptide comprising amino acids 1-205 of SEQ ID NO:18. 
     
     
         33 . The polypeptide of  claim 3 , wherein the polypeptide is a GH 61D polypeptide consisting of amino acids 1-205 of SEQ ID NO:18. 
     
     
         34 . The polypeptide of  claim 3 , wherein the polypeptide is a beta-glucosidase comprising an amino acid sequence which has at least 90% identity with a beta-glucosidase obtainable from  Botryosphaeria rhodina  deposited under deposit accession number CBS 274.96. 
     
     
         35 . The polypeptide of  claim 3 , wherein the polypeptide is a beta-glucosidase comprising amino acids 1 to 603 of SEQ ID NO: 34. 
     
     
         36 . The polypeptide of  claim 3 , wherein the polypeptide is a beta-glucosidase consisting of amino acids 1 to 603 of SEQ ID NO: 34. 
     
     
         37 . The polypeptide of  claim 3 , wherein the polypeptide is an endo-arabinase comprising an amino acid sequence which has at least 90% identity with a endo-arabinase obtainable from  Botryosphaeria rhodina  deposited under deposit accession number CBS 274.96. 
     
     
         38 . The polypeptide of  claim 3 , wherein the polypeptide is an endo-arabinase comprising amino acids 1 to 301 of SEQ ID NO: 36. 
     
     
         39 . The polypeptide of  claim 3 , wherein the polypeptide is an endo-arabinase consisting of amino acids 1 to 301 of SEQ ID NO: 36. 
     
     
         40 . The polypeptide of  claim 3 , wherein the polypeptide is an endo-arabinase comprising an amino acid sequence which has at least 90% identity with a endo-arabinase obtainable from  Botryosphaeria rhodina  deposited under deposit accession number CBS 274.96. 
     
     
         41 . The polypeptide of  claim 3 , wherein the polypeptide is an endo-arabinase comprising amino acids 1 to 438 of SEQ ID NO: 38. 
     
     
         42 . The polypeptide of  claim 3 , wherein the polypeptide is an endo-arabinase consisting of amino acids 1 to 438 of SEQ ID NO: 38. 
     
     
         43 . The polypeptide of  claim 3 , wherein the polypeptide is a pepsin peptidase comprising an amino acid sequence which has at least 90% identity with a pepsin peptidase obtainable from  Botryosphaeria rhodina  deposited under deposit accession number CBS 274.96. 
     
     
         44 . The polypeptide of  claim 3 , wherein the polypeptide is a pepsin peptidase comprising amino acids 1 to 396 of SEQ ID NO: 40. 
     
     
         45 . The polypeptide of  claim 3 , wherein the polypeptide is a pepsin peptidase consisting of amino acids 1 to 396 of SEQ ID NO: 40. 
     
     
         46 . The polypeptide of  claim 3 , wherein the polypeptide is a pepsin peptidase comprising an amino acid sequence which has at least 90% identity with a pepsin peptidase obtainable from  Botryosphaeria rhodina  deposited under deposit accession number CBS 274.96. 
     
     
         47 . The polypeptide of  claim 3 , wherein the polypeptide is a pepsin peptidase comprising amino acids 1 to 262 of SEQ ID NO: 42. 
     
     
         48 . The polypeptide of  claim 3 , wherein the polypeptide is a pepsin peptidase consisting of amino acids 1 to 262 of SEQ ID NO: 42. 
     
     
         49 . A polynucleotide comprising a nucleotide sequence which encodes for the polypeptide defined in  claim 3 . 
     
     
         50 . A nucleic acid construct comprising the nucleotide sequence defined in  claim 49  operably linked to one or more control sequences that direct the production of the polypeptide in a host cell. 
     
     
         51 . A recombinant expression vector comprising the nucleic acid construct of  claim 50 . 
     
     
         52 . A recombinant host cell comprising the nucleic acid construct of  claim 50 . 
     
     
         53 . A method for producing the polypeptide of  claim 3  comprising:
 (a) cultivating a strain, which in its wild-type form is capable of producing the polypeptide, to produce the polypeptide; and 
 (b) recovering the polypeptide. 
 
     
     
         54 . A method for producing a polypeptide of  claim 3  comprising:
 (a) cultivating a recombinant host cell as defined in  claim 52  under conditions conducive for production of the polypeptide; and 
 (b) recovering the polypeptide. 
 
     
     
         55 . A composition comprising the polypeptide of  claim 3  and an excipient. 
     
     
         56 . A method for preparing a composition of  claim 55  comprising admixing the polypeptide of  claim 1  with the excipient. 
     
     
         57 . A storage medium suitable for use in an electronic device comprising information of the amino acid sequence of the polypeptide of  claim 3 . 
     
     
         58 . A storage medium suitable for use in an electronic device comprising information of the nucleic acid sequence of the polynucleotide of  claim 49 .

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