Method of lipid assay and reagent for use therein

Abstract

A method of lipid assay characterized by assaying the lipids contained in a blood component in the presence of an organic silicon compound. The method can cause specific conditions for direct methods while satisfying requirements such as no influence on precision of assay, no burden on assay apparatus, and easy availability.

1 - 16 . (canceled) 
     
     
         17 . A fractionation method of assaying lipid components in a specific lipoprotein fraction, comprising assaying the lipid in blood components in the presence of an organic silicon compound. 
     
     
         18 . The method according to  claim 17 , wherein the organic silicon compound is a silicone, a derivative of silicone or a combination thereof. 
     
     
         19 . The method according to  claim 18 , wherein the silicone or the derivative of silicone is at least one member selected from the group consisting of high polymerization silicone, cyclic silicone, silicone-containing surfactant, and modified silicone oil. 
     
     
         20 . The method according to  claim 17 , wherein the lipid component is a cholesterol, triglycerides, or phospholipid. 
     
     
         21 . The method according to  claim 17 , wherein a lipid contained in the lipoprotein is detected by contacting the lipoprotein with an enzyme, under conditions in which the organic silicon compound is present and in the absence of a precipitation agent. 
     
     
         22 . The method according to  claim 21 , wherein the contacting step is performed in the presence of a surfactant having lipoprotein selectivity. 
     
     
         23 . A reagent for assaying lipid components in a specific lipoprotein fraction, comprising an organic silicon compound. 
     
     
         24 . The reagent according to  claim 23 , wherein the organic silicon compound is a silicone, a derivative of silicone or a combination thereof. 
     
     
         25 . The reagent according to  claim 24 , wherein the silicone or the derivative of silicone is at least one member selected from the group consisting of high polymerization silicone, cyclic silicone, silicone-containing surfactant, and modified silicone oil. 
     
     
         26 . The reagent according to  claim 23 , wherein the lipid component is a cholesterol, triglycerides, or phospholipid.