Method of regulating fertilizing ability using cyclic adp-ribose and cd38

Abstract

The present invention relates to a pharmaceutical composition for promoting fertilization comprising cyclic ADP-ribose or its derivative, CD 38 and to a method of promoting fertilization by promoting the synthesis of cyclic ADP-ribose to increase sperm motility. Also, the present invention relates to a pharmaceutical composition for contraception and a method for inhibiting fertilization, which can inhibit the expression or function of cyclic ADP-ribose to reduce sperm motility, thereby inhibiting fertilization.

1 . A pharmaceutical composition for promoting fertilization comprising cyclic ADP-ribose or its derivative. 
     
     
         2 . A pharmaceutical composition for promoting fertilization comprising CD38. 
     
     
         3 . The pharmaceutical composition of  claim 1 , wherein cyclic ADP-ribose is synthesized by CD38 (ADP-ribosyl cyclase). 
     
     
         4 . The pharmaceutical composition of  claim 1 , wherein CD38 is contained in prostasome. 
     
     
         5 . A method of promoting fertilization by increasing the motility of sperm, the method comprising a step of promoting the synthesis of cyclic ADP-ribose. 
     
     
         6 . The method of  claim 5 , wherein the cyclic ADP-ribose is synthesized in prostasome-bound sperm. 
     
     
         7 . The method of  claim 5 , wherein the method further comprises a step of treating the sperm with progesterone. 
     
     
         8 . The method of  claim 7 , wherein the treatment with the progesterone promotes intracellular calcium release to induce a continuous increase in calcium. 
     
     
         9 . A pharmaceutical composition for contraception comprising an antagonist of cyclic ADP-ribose. 
     
     
         10 . The pharmaceutical composition of  claim 9 , wherein the antagonist is 8-Br-cADPR or 8-amino-cADPR. 
     
     
         11 . The pharmaceutical composition of  claim 9 , wherein the cyclic ADP-ribose is synthesized in CD38 contained in prostasome. 
     
     
         12 . The pharmaceutical composition of  claim 9 , wherein the antagonist inhibits a pattern of a continuous increase in calcium by progesterone. 
     
     
         13 . A method for inhibiting fertilization comprising inhibiting cyclic ADP-ribose. 
     
     
         14 . The method of  claim 13 , wherein the inhibition is performed by treatment with an antagonist of the cyclic ADP-ribose. 
     
     
         15 . The pharmaceutical composition of  claim 10 , wherein the antagonist inhibits a pattern of a continuous increase in calcium by progesterone.