Composition for Preventing or Treating Liver Diseases, Containing Plant Stem Cell Lines Derived from the Cambium of Panax Ginseng Including Mountain Ginseng or Ginseng as Active Ingredient

Abstract

The present invention relates to a composition for preventing or treating liver diseases, which contains, as an active ingredient, any one or more of a homogeneous cell line derived from the cambium of Panax ginseng , including wild ginseng or ginseng, a lysate thereof, an extract thereof and a culture medium thereof. The homogeneous cell line according to the present invention, a lysate thereof, an extract thereof and a culture medium thereof have minimized side effects compared to existing agents for treating liver diseases, and thus are safe for the human body. Also, they can increase the levels of s-antibody (HBsAb) and e-antibody (HBeAb) against hepatitis virus and inhibit the proliferation of hepatitis virus, and thus they are useful for the prevention and treatment of liver diseases. In addition, they have the effect of lowering the levels of liver injury, and thus are useful as a functional food for improving liver function.

1 . A composition for preventing or treating liver diseases containing any one or more of a cell line, which is derived from a cambium of  Panax ginseng  and has the following characteristics; an extract thereof; a lysate thereof; and a culture thereof
 (a) it is in an innately undifferentiated state;   (b) it is a homogeneous cell line; and   (c) it is morphologically characterized by multiple vacuoles.   
     
     
         2 . The composition for preventing or treating liver diseases according to  claim 1 , wherein the cell line is additionally characterized in that:
 (a) it exists at single cell, level during suspension culture;   (b) it has low sensitivity to shear stress in a bioreactor compared to cell lines derived from tissues other than the cambium of  Panax ginseng ; and   (c) it has a higher growth rate and can be cultured more stably compared to the cell lines than those cell lines derived from tissues other than the cambium of  Panax ginseng.      
     
     
         3 . The composition for preventing or treating liver diseases according to  claim 1 , wherein the cell line is obtained using an isolation method comprising the following steps:
 (a) obtaining a tissue containing the cambium of  Panax ginseng;      (b) culturing the obtained cambium-containing tissue in a medium containing indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA), thereby inducing a cambium-derived cell line, wherein osmotic stress is applied to the cambium containing storage root before, during or after the culturing; and   (c) collecting the induced cambium-derived cell line.   
     
     
         4 . The composition for preventing or treating liver diseases according to any one among  claims 1  to  3 , wherein the  Panax ginseng  is wild ginseng or ginseng. 
     
     
         5 . The composition for preventing or treating liver diseases according to any one among  claims 1  to  3 , the liver diseases is any one among hepatitis, liver cancer, liver cirrhosis, fatty liver and toxipathic liver diseases. 
     
     
         6 . The composition for preventing or treating liver diseases according to  claim 1 , wherein the extract is obtained using a solvent selected from the group consisting of distilled water, alcohol, acetone, DMSO (dimethyl sulfoxide), PBS (phosphate buffered, saline) and mixed solvents thereof. 
     
     
         7 . A functional food for improving liver function containing any one or more of a cell line, which is derived from a cambium of  Panax ginseng  and has the following characteristics; an extract thereof; a lysate thereof; and a culture thereof:
 (a) it is in an innately undifferentiated state;   (b) it is a homogeneous cell line; and   (c) it is morphologically characterized by multiple vacuoles.   
     
     
         8 . The functional food for improving liver function according to  claim 7 , wherein the cell line is wherein the cell line is additionally characterized in that
 (a) it exists at single cell level during suspension culture;   (b) it has low sensitivity to shear stress in a bioreactor compared to cell lines derived from tissues other than the cambium of  Panax ginseng ; and   (c) it has a higher growth rate and can be cultured more stably compared to the cell lines than those cell lines derived from tissues other than the cambium of  Panax ginseng.      
     
     
         9 . The functional food for improving liver function according to  claim 7 , wherein the cell line is obtained using an isolation method comprising the following steps of:
 (a) obtaining a tissue containing the cambium of  Panax ginseng;      (b) culturing the obtained cambium-containing tissue in a medium containing indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA), thereby inducing a cambium-derived cell line, wherein osmotic stress is applied to the cambium containing storage root before, during or after the culturing; and   (c) collecting the induced cambium-derived cell line.   
     
     
         10 . A Composition for inhibiting proliferation of hepatitis virus containing any one or more of a cell line, which is derived from cambium of  Panax ginseng  and has the following characteristics; an extract thereof; a lysate thereof; and a culture thereof:
 (a) it is in an innately undifferentiated state;   (b) it is a homogeneous cell line; and   (c) it is morphologically characterized by multiple vacuoles.   
     
     
         11 . The composition for inhibiting proliferation of hepatitis virus according to  claim 10 , wherein the cell line is additionally characterized in that
 (a) it exists at single cell level during suspension culture;   (b) it has low sensitivity to shear stress in a bioreactor compared to cell lines derived from tissues other than the cambium of  Panax ginseng ; and   (c) it has a higher growth rate and can be cultured more stably compared to the cell lines than those cell lines derived from tissues other than the cambium of  Panax ginseng.      
     
     
         12 . The composition for inhibiting proliferation of hepatitis virus according to  claim 10 , wherein the cell line is obtained using an isolation method comprising the following steps of:
 (a) obtaining a tissue containing the cambium of  Panax ginseng;      (b) culturing the obtained cambium-containing tissue in a medium containing indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA), thereby inducing a cambium-derived cell line, wherein osmotic stress is applied to the cambium containing storage root before, during or after the culturing; and   (c) collecting the induced cambium-derived cell line.   
     
     
         13 . An immune-enhancing agent for increasing the level of antibody against hepatitis virus containing any one or more of a cell line, which is derived from a cambium of  Panax ginseng  and has the following characteristics; an extract thereof; a lysate thereof; and a culture thereof
 (a) it is in an innately undifferentiated state;   (b) it is a homogeneous cell line; and   (c) it is morphologically characterized by multiple vacuoles.   
     
     
         14 . The immune-enhancing agent for increasing the level of antibody against hepatitis virus according to  claim 13 , wherein the cell line is additionally characterized in that
 (a) it exists at single cell level during suspension culture;   (b) it has low sensitivity to shear stress in a bioreactor compared to cell lines derived from tissues other than the cambium of  Panax ginseng ; and   (c) it has a higher growth rate and can be cultured more stably compared to the cell lines than those cell lines derived from tissues other than the cambium of  Panax ginseng.      
     
     
         15 . The immune-enhancing agent for increasing the level of antibody against hepatitis virus according to  claim 13 , wherein the cell line is obtained using an isolation method comprising the following steps of:
 (a) obtaining a tissue containing the cambium of  Panax ginseng;      (b) culturing the obtained cambium-containing tissue in a medium containing indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA), thereby inducing a cambium-derived cell line, wherein osmotic stress is applied to the cambium containing storage root before, during or after the culturing; and   (c) collecting the induced cambium-derived cell line.