US2012196372A1PendingUtilityA1

Methods for Reducing Gluconoylation of Proteins

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Assignee: GARDNER ALAN RPriority: Mar 4, 2003Filed: Jan 31, 2012Published: Aug 2, 2012
Est. expiryMar 4, 2023(expired)· nominal 20-yr term from priority
Inventors:Alan R. GardnerThomas D. SweitzerAlexander H. TaylorPramathesh S. Patel
C12P 21/00C12N 9/18C07K 14/54C12N 1/00
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Claims

Abstract

The present invention relates to methods of preventing glyconoylation of polypeptides produced in micororganisms.

Claims

exact text as granted — not AI-modified
1 .- 15 . (canceled) 
     
     
         16 . A method for preventing gluconoylation of polypeptides expressed in a microorganism, comprising introducing DNA into the microorganism, wherein the DNA encodes a polypeptide that demonstrates 6-phosphogluconolactonase activity. 
     
     
         17 . The method of  claim 16 , wherein the microorganism is  E. coli.    
     
     
         18 . The method of  claim 16 , wherein the DNA comprises DNA encoding a 6-phosphogluconolactonase enzyme. 
     
     
         19 . The method of  claim 18 , wherein the DNA encoding the 6-phosphogluconolactonase enzyme has at least 70% sequence identity to DNA encoding 6-phosphogluconolactonase from  Pseudomonas.    
     
     
         20 . The method of  claim 19 , wherein the  Pseudomonas  is  P. aeruginosa.    
     
     
         21 . The method of  claim 18 , wherein the DNA encoding the 6-phosphogluconolactonase enzyme has at least 90% sequence identity to SEQ ID NO:7. 
     
     
         22 . The method of  claim 18 , wherein the DNA encoding the 6-phosphogluconolactonase enzyme has at least 95% sequence identity to SEQ ID NO:7. 
     
     
         23 . The method of  claim 18 , wherein the 6-phosphogluconolactonase enzyme has an amino acid sequence having at least 90% sequence identity to SEQ ID NO:8. 
     
     
         24 . The method of  claim 18 , wherein the 6-phosphogluconolactonase enzyme has an amino acid sequence having at least 95% sequence identity to SEQ ID NO:8. 
     
     
         25 . The method of  claim 18 , wherein the DNA encoding the 6-phosphogluconolactonase enzyme comprises the sequence set forth in SEQ ID NO:7. 
     
     
         26 . The method of  claim 16 , wherein the DNA is recombinant DNA. 
     
     
         27 . The method of  claim 16 , wherein the DNA is transformed into the genomic DNA of the microorganism. 
     
     
         28 .- 59 . (canceled)

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