Method for producing transgenic surface layer chimeric plant

Abstract

A rose is produced in which an introduced gene is only present in a part of the cells thereof, such as cells of the L1 layer of flower petals, but is not present in germ cells such as pollen cells or ovule cells. Since the introduced gene is not propagated to other roses even when this rose is crossed with other roses, the possibility of dispersal of the introduced gene can be completely negated.

1 : A floricultural plant that contains a foreign gene only in a part of the cells thereof, but not in other cells. 
     
     
         2 : The floricultural plant according to  claim 1 , wherein the part of the cells comprise a part of a cell layer. 
     
     
         3 : The floricultural plant according to  claim 1 , wherein the other cells are pollen cells or ovule cells. 
     
     
         4 : The floricultural plant according to  claim 1 , wherein the other cells are cells comprising the L2 layer. 
     
     
         5 : The floricultural plant according to  claim 2 , wherein the part of the cell layer is either the L1 layer or the L1 layer and the L3 layer. 
     
     
         6 : The floricultural plant according to  claim 2 , wherein the floricultural plant is a floricultural plant into which a foreign gene is able to be introduced mediated by  Agrobacterium.    
     
     
         7 : The floricultural plant according to  claim 6 , wherein the floricultural plant is any of rose, carnation, petunia, torenia, tobacco, verbena, nierembergia, chrysanthemum, lily, morning glory, common snapdragon, cyclamen, orchid, prairie gentian, freesia, gerbera, gladiola, baby's breath, kalanchoe, pelargonium, geranium, tulip, rapeseed, potato, tomato, poplar, banana, eucalyptus, sweet potato, soybean, alfalfa, lupine and cauliflower. 
     
     
         8 : The floricultural plant according to  claim 1 , wherein the foreign gene is a gene that functions in L1 layer cells following introduction. 
     
     
         9 : The floricultural plant according to  claim 8 , wherein the foreign gene is a gene relating to flower color. 
     
     
         10 : The floricultural plant according to  claim 9 , wherein the foreign gene is a gene involved in flavonoid synthesis. 
     
     
         11 : The floricultural plant according to  claim 10 , wherein the foreign gene is one or a plurality of flavonoid hydroxylase gene derived from a plant belonging to the violet family, aromatic acyl group transferase gene derived from a plant belonging to the snapdragon family, flavonoid 3′,5′-hydroxylase gene cDNA derived from salvia, genomic gene of petunia dihydroflavonol 4-reductase, and anthocyanin synthase gene derived from carnation. 
     
     
         12 : The floricultural plant according to  claim 8 , wherein the foreign gene is a selectable marker gene or a gene encoding a transcription factor. 
     
     
         13 : The floricultural plant according to  claim 7 , wherein the floricultural plant is a rose. 
     
     
         14 : The floricultural plant according to  claim 13 , wherein the rose is Rosa hybrida. 
     
     
         15 : The floricultural plant according to  claim 14 , wherein the rose is hybrid tea rose, floribunda or miniature rose. 
     
     
         16 : The floricultural plant according to  claim 13 , wherein the foreign gene is a gene relating to flower color. 
     
     
         17 . The floricultural plant according to  claim 13 , wherein the foreign gene is a gene involved in flavonoid synthesis. 
     
     
         18 : The floricultural plant according to  claim 17 , wherein the foreign gene is flavonoid 3′,5′-hydroxylase gene derived from a plant belonging to the violet family and/or aromatic acyl group transferase gene derived from a plant belonging to the snapdragon family. 
     
     
         19 : The floricultural plant according to  claim 18 , wherein the plant belonging to the violet family is a pansy. 
     
     
         20 : The floricultural plant according to  claim 18 , wherein the plant belonging to the snapdragon family is a torenia. 
     
     
         21 : The floricultural plant according to  claim 13 , wherein flower color changes to a bluish color as compared with a rose prior to gene introduction. 
     
     
         22 : The floricultural plant according to  claim 7 , wherein the floricultural plant is a carnation. 
     
     
         23 : The floricultural plant according to  claim 22 , wherein the carnation is of the spray type or standard type. 
     
     
         24 : The floricultural plant according to  claim 23 , wherein the foreign gene is a gene relating to flower color. 
     
     
         25 : The floricultural plant according to  claim 24 , wherein the foreign gene is a gene of an enzyme involved in flavonoid synthesis. 
     
     
         26 : The floricultural plant according to  claim 25 , wherein the foreign gene is one or a plurality of flavonoid 3′,5′-hydroxylase gene cDNA derived from salvia, genomic gene of petunia dihydroflavonol 4-reductase, and anthocyanin synthase gene derived from carnation. 
     
     
         27 : The floricultural plant according to  claim 22 , wherein flower color changes to a bluish color as compared with a carnation prior to gene introduction. 
     
     
         28 : A tissue having the same properties as the floricultural plant according to  claim 1 , or a vegitative propagation product thereof. 
     
     
         29 : A method for producing the floricultural plant according to  claim 1 , comprising a step for introducing a foreign gene into a plant mediated by  Agrobacterium , and selecting a plant in which the foreign gene is only present in a part of the cells thereof. 
     
     
         30 : A method for preventing dispersal of an introduced gene into the natural world by producing the floricultural plant not having a foreign gene in the germ cells thereof according to  claim 3 .