Methods and products for manipulating hematopoietic stem cells

Abstract

The invention relates to methods for manipulating hematopoietic stem cells and related products. In one aspect the invention relates to the use of stem cell G-protein coupled receptor (SC-GPR) related compositions to identify bone marrow derived hematopoietic stem cells, to enhance mobilization of hematopoietic stem cells, to improve the efficiency of targeting cells to the bone marrow and/or to modulate hematopoietic cell function.

1 . A method for promoting homing of hematopoietic cells to bone marrow, comprising administering to a subject a hematopoietic cell that expresses an SC-GPR polypeptide, wherein the hematopoietic cells home to the bone marrow. 
     
     
         2 . The method of  claim 1 , wherein the hematopoietic cells are autologous to the subject. 
     
     
         3 . The method of  claim 1 , wherein the hematopoietic cells are allogeneic to the subject. 
     
     
         4 . The method of  claim 1 , wherein the hematopoietic cells are isolated from cord blood. 
     
     
         5 . The method of  claim 1 , wherein the hematopoietic cells are CD34+ hematopoietic cells. 
     
     
         6 . The method of  claim 1 , wherein the hematopoietic cells are hematopoietic progenitor cells. 
     
     
         7 . The method of  claim 1 , wherein the hematopoietic cells are hematopoietic stem cells. 
     
     
         8 . The method of  claim 1 , wherein the hematopoietic cells are not hematopoietic stem cells. 
     
     
         9 . The method of  claim 1 , wherein the hematopoietic cells are human hematopoietic cells. 
     
     
         10 . The method of  claim 1 , wherein the hematopoietic cells are expanded in vitro or ex vivo. 
     
     
         11 . The method of  claim 1 , wherein the SC-GPR polypeptide comprises any one of SEQ ID NOs: 1 to 3. 
     
     
         12 . The method of  claim 1 , wherein the hematopoietic cell is transduced with a polynucleotide encoding the SC-GPR polypeptide. 
     
     
         13 . The method of  claim 1 , wherein subject is a bone marrow donor or is in need of a bone marrow transplant. 
     
     
         14 . The method of  claim 1 , wherein the subject has received or is receiving chemotherapy. 
     
     
         15 . A method for modulating hematopoietic cell function, comprising introducing a polynucleotide encoding an SC-GPR polypeptide into hematopoietic cell, thereby modulating a hematopoietic cell function. 
     
     
         16 . The method of  claim 15 , wherein the modulated hematopoietic cell function is chemotaxis. 
     
     
         17 . The method of  claim 15 , wherein the modulated hematopoietic cell function is homing to the bone marrow. 
     
     
         18 . The method of  claim 15 , wherein the polynucleotide encodes an SC-GPR polypeptide as set forth in any one of SEQ ID NOs: 1 to 3. 
     
     
         19 . The method of  claim 15 , wherein the hematopoietic cell is transduced with the polynucleotide encoding the SC-GPR polypeptide. 
     
     
         20 . A method of increasing migration of hematopoietic cells to the bone marrow, comprising administering to a subject, a hematopoietic cell that expresses an exogenous SC-GPR polypeptide.