Rapidly acting dry sealant and methods for use and manufacture

Abstract

Compositions, methods, and kits are provided for sealing applications. Compositions are prepared by combining a first cross-linkable component with a second cross-linkable component to form a porous matrix having interstices, and combining the porous matrix with a hydrogel-forming component to fill at least some of the interstices. The compositions exhibit minimal swelling properties.

1 . A sealant composition for use in treating an individual, comprising:
 a porous layer,   a first component associated with the porous layer, the first component comprising a nucleophilic polymer, and   a second component associated with the porous layer, the second component comprising an electrophilic polymer.   
     
     
         2 . The sealant composition according to  claim 1 , wherein each of the first and second components is present as a gel. 
     
     
         3 . The sealant composition according to  claim 1 , wherein the porous layer comprises a member selected from the group consisting of a pad, a sheet, a film, and a sponge. 
     
     
         4 . The sealant composition according to  claim 1 , wherein the porous layer comprises a member selected from the group consisting of collagen, fibrin, cellulose, and chitosan. 
     
     
         5 . The sealant composition according to  claim 1 , wherein the first component nucleophilic polymer comprises a member selected from the group consisting of a dilysine, a trilysine, a quatralysine, a pentalysine, a dicysteine, a tricysteine, a quatracysteine, a pentacystein, and an oligopeptide or polypeptide comprising two or more lysines or cysteines. 
     
     
         6 . The sealant composition according to  claim 1 , wherein the second component electrophilic polymer comprises two or more succinimidyl groups. 
     
     
         7 . The sealant composition according to  claim 1 , wherein the second component comprises a polyethylene glycol containing a succinimidyl group. 
     
     
         8 . A method of treating a tissue of an individual, the method comprising:
 applying a sealant composition to the tissue of the patient, the sealant composition comprising a first component having a nucleophilic polymer and a second component having an electrophilic polymer, and wherein the first component and the second component are fixed on a support comprising a member selected from the group consisting of a protein, a carbohydrate, and a synthetic polymer.   
     
     
         9 . The method according to  claim 8 , wherein each of the first and second components is present as a gel. 
     
     
         10 . The method according to  claim 8 , wherein the sealant composition further comprises a hydrogel-forming component fixed on the support. 
     
     
         11 . The method according to  claim 10 , wherein the hydrogel forming component comprises a biologic polymer selected from the group consisting of gelatin, collagen, albumin, hemoglobin, fibrogen, fibrin, casein, fibronectin, elastin, keratin, laminin, and derivatives and combinations thereof. 
     
     
         12 . The method according to  claim 8 , wherein the support is porous and comprises a member selected from the group consisting of a pad, a sheet, a film, and a sponge. 
     
     
         13 . The method according to  claim 8 , wherein the support comprises a member selected from the group consisting of collagen, fibrin, cellulose, and chitosan. 
     
     
         14 . The method according to  claim 8 , wherein the first component nucleophilic polymer comprises a member selected from the group consisting of a dilysine, a trilysine, a quatralysine, a pentalysine, a dicysteine, a tricysteine, a quatracysteine, a pentacystein, and an oligopeptide or polypeptide comprising two or more lysines or cysteines. 
     
     
         15 . The method according to  claim 8 , wherein the second component electrophilic polymer comprises two or more succinimidyl groups. 
     
     
         16 . The method according to  claim 8 , wherein the second component comprises a polyethylene glycol containing a succinimidyl group. 
     
     
         17 . The method according to  claim 8 , wherein the sealant composition further comprises an active agent. 
     
     
         18 . The method according to  claim 8 , wherein the sealant composition further comprises a hemostatic agent selected from the group consisting of thrombin, fibrinogen, and a clotting agent. 
     
     
         19 . A method of making a sealant composition for use in treating a tissue of an individual, the method comprising:
 obtaining a porous layer; and   applying a mixture to the porous layer, the mixture comprising a first component having a nucleophilic polymer and a second component having an electrophilic polymer.   
     
     
         20 . The method according to  claim 19 , wherein each of the first and second components is present as a gel. 
     
     
         21 . The method according to  claim 19 , wherein the porous layer comprises collagen. 
     
     
         22 . The method according to  claim 19 , wherein the first component nucleophilic polymer comprises a member selected from the group consisting of a dilysine, a trilysine, a quatralysine, a pentalysine, a dicysteine, a tricysteine, a quatracysteine, a pentacystein, and an oligopeptide or polypeptide comprising two or more lysines or cysteines, and wherein the second component comprises a polyethylene glycol containing a succinimidyl group.