Stirring method, cell culture method, stirring apparatus, and cell culture apparatus

Abstract

A stirring method includes measuring, with respect to cells which adhere to an inner surface of a culture vessel to be cultured, an ease of peeling between the culture vessel and the cell and an ease of peeling between the mutual cells, and performing a stirring process on the cells in the culture vessel by determining a process content of the stirring process with respect to the cells in the culture vessel, based on the ease of peeling between the culture vessel and the cell and the ease of peeling between the mutual cells.

1 . A stirring method, comprising:
 measuring, with respect to cells which adhere to an inner surface of a culture vessel to be cultured, an ease of peeling between the culture vessel and the cell and an ease of peeling between the mutual cells; and   performing a stirring process on the cells in the culture vessel by determining a process content of the stirring process with respect to the cells in the culture vessel, based on the ease of peeling between the culture vessel and the cell and the ease of peeling between the mutual cells.   
     
     
         2 . A cell culture method, comprising:
 seeding the cells in the culture vessel; and   stirring the cells cultured in the culture vessel, by using the stirring method according to  claim 1 .   
     
     
         3 . The cell culture method according to  claim 2 , wherein
 the measuring measures the ease of peeling between the culture vessel and the cell and the ease of peeling between the mutual cells comprises:   measuring the ease of peeling between the culture vessel and the cell by transporting the culture vessel at a predetermined acceleration and stopping the culture vessel, when the cells are seeded in the culture vessel and the cells are fixed to the culture vessel; and   measuring the ease of peeling between the mutual cells by transporting the culture vessel at a predetermined acceleration and stopping the culture vessel after the cells are cultured in the culture vessel.   
     
     
         4 . A stirring apparatus, comprising:
 a stirring unit executing a stirring process on cells which adhere to an inner surface of a culture vessel to be cultured;   a stirring process content determining unit determining a process content of the stirring process in the stirring unit, in accordance with a magnitude relation between an ease of peeling between the cell and the inner surface of the culture vessel and an ease of peeling between the mutual cells; and   a control unit drive-controlling the stirring unit based on the process content of the stirring process determined by the stirring process content determining unit.   
     
     
         5 . The stirring apparatus according to  claim 4 , further comprising
 an input unit inputting a type of the culture vessel and a type of the cell, wherein   the stirring process content determining unit estimates the magnitude relation based on the type of the culture vessel and the type of the cell input by the input unit, and determines the content of the stirring process in the stirring unit.   
     
     
         6 . The stirring apparatus according to  claim 5 , further comprising
 a storage unit storing stirring process information to which the process content of the stirring process is corresponded, in accordance with the type of the culture vessel and the type of the cell, wherein   the stirring process content determining unit determines the process content of the stirring process in the stirring unit by refereeing to the stirring process information when the type of the culture vessel and the type of the cell are input by the input unit.   
     
     
         7 . The stirring apparatus according to  claim 4 , further comprising:
 a culture vessel transporting unit giving an acceleration to the culture vessel;   an observing unit observing movements of cells in the culture vessel; and   an analyzing unit determining the magnitude relation by analyzing an observation result of the cells obtained by the observing unit.   
     
     
         8 . The stirring apparatus according to  claim 4 , wherein
 the magnitude relation is determined as a magnitude relation between an adhesive strength between the cell and the inner surface of the culture vessel and an adhesive strength between the mutual cells.   
     
     
         9 . The stirring apparatus according to  claim 7 , wherein
 the magnitude relation is determined as a magnitude relation between a moving distance when the cell adhered to the culture vessel is peeled off from the culture vessel when applying the acceleration and a moving distance of the cell of the mutually adhered cells when the mutual cells are peeled off from each other.   
     
     
         10 . The stirring apparatus according to  claim 4 , wherein
 the process content of the stirring process is formed of the presence/absence of the stirring process, the acceleration given to the culture vessel when executing the stirring process and a number of times of giving the acceleration.   
     
     
         11 . A cell culture apparatus, comprising:
 the stirring apparatus according to  claims 4 ; and   a temperature-controlled room including the stirring apparatus in an inside thereof and maintaining an environment to a state of culturing the cells.   
     
     
         12 . A stirring apparatus, comprising:
 a stirring unit executing a stirring process with respect to cells which adhere to an inner surface of a culture vessel to be cultured;   an input unit inputting a type of the culture vessel and a type of the cell;   a storage unit storing a process content of the stirring process determined based on previously determined ease of peeling between the culture vessel and the cell and ease of peeling between the mutual cells from a combination of the type of the culture vessel and the type of the cell input by the input unit; and   a control unit drive-controlling the stirring unit based on the process content of the stirring process read from the storage unit based on the type of the culture vessel and the type of the cell.   
     
     
         13 . A cell culture apparatus, comprising:
 the stirring apparatus according to  claim 12 ; and   a temperature-controlled room including the stirring apparatus in an inside thereof and maintaining an environment to a state of culturing the cells.