Methods For Monitoring Multiple Gene Expression

Abstract

The present invention relates to methods for monitoring differential expression of a plurality of genes in a first Bacillus cell relative to expression of the same genes in one or more second Bacillus cells using microarrays containing Bacillus genomic sequenced tags. The present invention also relates to computer readable media and computer-based systems. The present invention further relates to substrates containing an array of Bacillus licheniformis or Bacillus clausii GSTs.

1 . A mutant  Bacillus  cell comprising a disruption or deletion of a gene encoding a YwsC protein, wherein the gene sequence comprises a sequence having at least 85% sequence homology to SEQ ID NO: 2694. 
     
     
         2 . The mutant  Bacillus  cell of  claim 1 , wherein the gene sequence comprises a sequence having at least 90% sequence homology to SEQ ID NO: 2694. 
     
     
         3 . The mutant  Bacillus  cell of  claim 1 , wherein the gene sequence comprises a sequence having at least 95% sequence homology to SEQ ID NO: 2694. 
     
     
         4 . The mutant  Bacillus  cell of  claim 1 , wherein the gene sequence comprises the sequence of SEQ ID NO: 2694. 
     
     
         5 . The mutant  Bacillus  cell of  claim 1 , wherein the cell is a  Bacillus licheniformis  cell. 
     
     
         6 . The mutant  Bacillus  cell of  claim 1 , wherein the cell exhibits reduced expression of the gene. 
     
     
         7 . A mutant  Bacillus  cell comprising a disruption or deletion of a gene encoding a YwsC protein, wherein the gene sequence comprises a sequence that hybridizes under medium-high stringency conditions to the complementary strand of the sequence of SEQ ID NO: 2694. 
     
     
         8 . The mutant  Bacillus  cell of  claim 7 , wherein the gene sequence comprises a sequence that hybridizes under high stringency conditions to the complementary strand of the sequence of SEQ ID NO: 2694. 
     
     
         9 . The mutant  Bacillus  cell of  claim 7 , wherein the gene sequence comprises a sequence that hybridizes under very-high stringency conditions to the complementary strand of the sequence of SEQ ID NO: 2694. 
     
     
         10 . The mutant  Bacillus  cell of  claim 7 , wherein the cell is a  Bacillus licheniformis  cell. 
     
     
         11 . The mutant  Bacillus  cell of  claim 7 , wherein the cell exhibits reduced expression of the gene. 
     
     
         12 . An isolated DNA which encodes a YwsC protein, wherein the isolated DNA sequence comprises a sequence having at least 85% homology to SEQ ID NO: 2694. 
     
     
         13 . The isolated DNA of  claim 12 , wherein the isolated DNA sequence comprises a sequence having at least 90% homology to SEQ ID NO: 2694. 
     
     
         14 . The isolated DNA of  claim 12 , wherein the isolated DNA sequence comprises a sequence having at least 95% homology to SEQ ID NO: 2694. 
     
     
         15 . The isolated DNA of  claim 12 , wherein the isolated DNA sequence comprises a sequence having SEQ ID NO: 2694. 
     
     
         16 . An isolated YwsC protein encoded by the DNA sequence of  claim 12 . 
     
     
         17 . A transformant cell comprising the isolated DNA sequence of  claim 12 . 
     
     
         18 . The transformant cell of  claim 17 , wherein the cell exhibits increased YwsC production. 
     
     
         19 . The transformant cell of  claim 17 , wherein the cell is a  Bacillus  cell. 
     
     
         20 . The transformant cell of  claim 17 , wherein the cell is a  Bacillus licheniformis  cell. 
     
     
         21 . An isolated DNA which encodes a YwsC protein, wherein the isolated DNA sequence comprises a sequence that hybridizes under medium-high stringency conditions to the complementary strand of the sequence of SEQ ID NO: 2694. 
     
     
         22 . The isolated DNA of  claim 21 , wherein the isolated DNA sequence comprises a sequence that hybridizes under high stringency conditions to the complementary strand of the sequence of SEQ ID NO: 2694. 
     
     
         23 . The isolated DNA of  claim 21 , wherein the isolated DNA sequence comprises a sequence that hybridizes under very-high stringency conditions to the complementary strand of the sequence of SEQ ID NO: 2694. 
     
     
         24 . An isolated YwsC protein encoded by the DNA sequence of  claim 21 . 
     
     
         25 . A transformant cell comprising the isolated DNA sequence of  claim 21 . 
     
     
         26 . The transformant cell of  claim 25 , wherein the cell exhibits increased YwsC production. 
     
     
         27 . The transformant cell of  claim 25 , wherein the cell is a  Bacillus  cell. 
     
     
         28 . The transformant cell of  claim 25 , wherein the cell is a  Bacillus licheniformis  cell.