Fluorescence analyzing method, fluorescence analyzing apparatus and image detecting method

Abstract

A fluorescence analyzing method includes the steps of irradiating a board, to which oligonucleotide is fixed, with light for fluorescence measurement; focusing produced fluorescence to form an image; and detecting the fluorescence with a two-dimensional sensor. Here, the board is provided with plural regions to which the oligonucleotide is fixed, and the plural regions are spaced apart from one another on the board substantially equidistantly in the vertical and horizontal directions. A fluorescent image is detected in a condition where the following equation is satisfied: dd=ds×M/n where ds denotes the interval between the regions, M denotes the imaging magnification of an optical focusing/imaging system, dd denotes the pixel pitch of the two-dimensional sensor, and n denotes an integer (n=1, 2, 3, 4, 5).

1 - 10 . (canceled) 
     
     
         11 . A fluorescence analysis apparatus for detecting fluorescence with a two-dimensional sensor by irradiating a board, to which oligonucleotide is fixed, with light for fluorescence measurement, and then focusing the produced fluorescence to form an image, comprising:
 a board provided with a plurality of regions to which the oligonucleotide is fixed, and which are spaced apart from one another substantially equidistantly in the vertical and horizontal directions;   a light source of excitation light for fluorescence;   an optical irradiating system of excitation light for fluorescence;   an optical focusing/imaging system for fluorescence; and   a two-dimensional sensor,   wherein the fluorescence analyzing apparatus is configured in a way that the following equation is satisfied:
     dd=ds×M/n    
   
       where ds denotes the interval between the regions, M denotes the imaging magnification of the optical focusing/imaging system, dd denotes the pixel pitch of the two-dimensional sensor, and n denotes an integer (n=1, 2, 3, 4, 5). 
     
     
         12 . A fluorescence analysis apparatus for detecting fluorescence with a two-dimensional sensor by irradiating a board, to which oligonucleotide is fixed, with light for fluorescence measurement, and then focusing the produced fluorescence to form an image, comprising:
 a board provided with a plurality of regions to which the oligonucleotide is fixed, and which are spaced apart from one another substantially equidistantly in the vertical and horizontal directions;   a light source of excitation light for fluorescence;   an optical irradiating system of excitation light for fluorescence;   an optical focusing/imaging system for fluorescence; and   a two-dimensional sensor,   wherein the fluorescence analyzing apparatus is configured in a way that the following equation is satisfied:
     dd=ds×M/n    
   
       where ds denotes the interval between the regions, M denotes the imaging magnification of the optical focusing/imaging system, dd denotes the pixel pitch of the two-dimensional sensor, and n denotes an integer (n=2, 3). 
     
     
         13 . A fluorescence analysis apparatus for detecting fluorescence with a two-dimensional sensor by irradiating a board, to which oligonucleotide is fixed, with light for fluorescence measurement, and then focusing the produced fluorescence to form an image, comprising:
 a board provided with a plurality of regions to which the oligonucleotide is fixed, and which are spaced apart from one another substantially equidistantly in the vertical and horizontal directions;   a light source of excitation light for fluorescence;   an optical irradiating system of excitation light for fluorescence;   an optical focusing/imaging system for fluorescence;   a two-dimensional sensor; and   a mechanism for automatically adjusting an imaging magnification to satisfy the following equation:
     dd=ds×M/n    
   
       where ds denotes the interval between the regions, M denotes the imaging magnification of the optical focusing/imaging system, dd denotes a pixel pitch of the two-dimensional sensor, and n denotes an integer (n=1, 2, 3, 4, 5). 
     
     
         14 . (canceled)