Organic colored microparticles, diagnostic reagent kit containing the same, and in vitro diagnosis method

Abstract

Provided are an immunochromatography kit that is highly sensitive and capable of multicoloration, and organic colored microparticles that are ideal as an element of the immunochromatography kit. Organic colored microparticles having an average grain size between 10 and 1,000 nm and a color intensity between 1.0 and 5.0 are prepared using cellulose as the starting material. When the organic colored microparticles are used as a label in an immunochromatography kit, the immunochromatography kit is of a high sensitivity than conventional technology. The immunochromatography kit is also capable of multicoloration and is useful for rapid diagnosis.

1 . Organic colored microparticles having an average particle size of 10 nm to 1000 nm and a color intensity of 1.0 to 5.0. 
     
     
         2 . The organic colored microparticles according to  claim 1 , wherein 10% by weight to 80% by weight of the organic colored microparticles constitute a coloring component. 
     
     
         3 . The organic colored microparticles according to  claim 2 , wherein the colored component is a dye. 
     
     
         4 . The organic colored microparticles according to  claim 1 , wherein 20% by weight to 90% by weight of the organic colored microparticles is derived from cellulose. 
     
     
         5 . The organic colored microparticles according to  claim 1 , wherein a ligand is bound by physical adsorption. 
     
     
         6 . The organic colored microparticles according to  claim 1 , having a reactive group. 
     
     
         7 . The organic colored microparticles according to  claim 6 , wherein the reactive group has a spacer structure having 3 or more atoms. 
     
     
         8 . The organic colored microparticles according to  claim 6 , wherein a ligand is bound to the reactive group by covalent bonding. 
     
     
         9 . A diagnostic reagent kit containing the organic colored microparticles according to any of  claims 1  to  8 . 
     
     
         10 . The diagnostic reagent kit according to  claim 9 , which is an immunochromatography kit. 
     
     
         11 . An in vitro diagnosis method comprising a step of using the organic colored microparticles according to any of  claims 1  to  8 . 
     
     
         12 . The in vitro diagnostic method according to  claim 11 , which is an immunochromatography method.