Biologically Active Human Umbilical Cord Blood Cell Extract Compounds and Methods

Abstract

Compositions and methods for culturing therapeutic cells are provided herein. According to at least one embodiment, compositions comprising cord blood plasma and lysed platelets and methods for making and using same are provided herein.

1 . A composition for culturing therapeutic cells, the composition comprising human platelet lysate and plasma derived from cord blood, wherein the concentration of platelet lysate is approximately the equivalent of 1×10 9  platelets/ml of composition, and wherein the plasma and/or platelets are operable to culture one or more therapeutic cells. 
     
     
         2 . The composition of  claim 1 , further comprising approximately 0.5 mM/mL to about 5 mM/mL calcium chloride. 
     
     
         3 . The composition of  claim 1 , wherein the concentration of platelet lysate is approximately the equivalent of 0.5×10 9  platelets/ml of composition. 
     
     
         4 . The composition of  claim 1 , wherein the concentration of platelet lysate is approximately the equivalent of 0.5×10 9  platelets/ml of composition. 
     
     
         5 . The composition of  claim 3 , wherein the composition is operable to proliferate human mesenchymal stem cells or dental papillary stem cells. 
     
     
         6 . The composition of  claim 3 , wherein the composition is operable to proliferate human stem cells at a rate less than double that of fetal bovine serum under identical conditions. 
     
     
         7 . The composition of  claim 4 , wherein the composition is operable to proliferate human stem cells at a rate less than four times that of fetal bovine serum under identical conditions. 
     
     
         8 . The composition of  claim 2 , further comprising heparin. 
     
     
         9 . A method for culturing therapeutic cells comprising the steps of:
 a. providing a therapeutic cell;   b. providing a composition derived from cord blood consisting essentially of combined human platelet lysate and plasma, wherein the concentration of platelet lysate is approximately the equivalent of 1×10 9  platelets/ml of composition;   c. culturing the therapeutic cell on the composition.   
     
     
         10 . The method of  claim 9 , wherein the composition further comprises approximately 0.5 mM/mL to about 5 mM/mL calcium chloride. 
     
     
         11 . The method of  claim 9 , wherein the concentration of platelet lysate is approximately the equivalent of 0.5×109 platelets/ml of composition. 
     
     
         12 . The method of  claim 9 , wherein the concentration of platelet lysate is approximately the equivalent of 0.5×109 platelets/ml of composition. 
     
     
         13 . The method of  claim 9 , wherein the therapeutic cell is mesenchymal stem cells or dental papillary stem cells. 
     
     
         14 . The method of  claim 13 , wherein the therapeutic cell is mesenchymal stem cells or dental papillary stem cells. 
     
     
         15 . The method of  claim 9 , further comprising the step of diluting the platelet lysate with additional plasma such that the resulting concentration is between about half to about one quarter of the original concentration. 
     
     
         16 . The method of  claim 15 , wherein the step of culturing the therapeutic cell on the composition occurs at a rate of less than four times that of a therapeutic cell cultured at the original concentration. 
     
     
         17 . A composition for culturing therapeutic cells, the composition consisting essentially of human platelet lysate and plasma derived from cord blood, wherein the concentration of platelet lysate is approximately the equivalent of 1×10 9  platelets/ml of composition, and wherein the plasma and/or platelets are operable to culture one or more therapeutic cells. 
     
     
         18 . The composition of  claim 18 , further consisting of calcium chloride. 
     
     
         19 . The composition of  claim 19 , further consisting of heparin.