Zcytor17 heterodimeric cytokine receptor polynucleotides

Abstract

Novel polypeptide combinations, polynucleotides encoding the polypeptides, and related compositions and methods are disclosed for zcytor17-containing multimeric or heterodimer cytokine receptors that may be used as novel cytokine antagonists, and within methods for detecting ligands that stimulate the proliferation and/or development of hematopoietic, lymphoid and myeloid cells in vitro and in vivo. The present invention also includes methods for producing the multimeric or heterodimeric cytokine receptor, uses therefor and antibodies thereto.

1 . An isolated polynucleotide that encodes a cytokine receptor polypeptide comprising amino acid residues 20-649 of SEQ ID NO:109, wherein the cytokine receptor polypeptide forms a heterodimeric cytokine receptor with a second cytokine receptor polypeptide comprising amino acid residues 28-979 of SEQ ID NO:7, and wherein the heterodimeric cytokine receptor binds a ligand comprising amino acid residues 27-164 of SEQ ID NO:2. 
     
     
         2 . An expression vector comprising the following operably linked elements:
 a transcription promoter;   a DNA segment encoding a cytokine receptor polypeptide comprising amino acid residues 20-649 of SEQ ED NO:109; and   a transcription terminator;   wherein the cytokine receptor polypeptide forms a heterodimeric cytokine receptor with a second cytokine receptor polypeptide comprising amino acid residues 28-979 of SEQ ID NO:7, and wherein the heterodimeric cytokine receptor binds a ligand comprising amino acid residues 27-164 of SEQ ID NO:2.   
     
     
         3 . An expression vector comprising the following operably linked elements:
 a) a first transcription promoter; a first DNA segment encoding a first cytokine receptor polypeptide comprising amino acid residues 20-649 of SEQ ID NO:109; and a first transcription terminator; and   b) a second transcription promoter; a second DNA segment encoding a second cytokine receptor polypeptide comprising amino acid residues 28-979 of SEQ ID NO:7; and a second transcription terminator;   wherein the first cytokine receptor polypeptide and the second cytokine receptor polypeptide form a heterodimeric cytokine receptor; and   wherein the heterodimeric cytokine receptor binds to a ligand comprising amino acid residues 27-164 of SEQ ID NO:2.   
     
     
         4 . A cultured cell comprising the expression vector of  claim 2 , wherein the cell expresses the cytokine receptor polypeptide encoded by the DNA segment. 
     
     
         5 . A cultured cell comprising the expression vector of  claim 3 , wherein the cell expresses the first cytokine receptor polypeptide and the second cytokine receptor polypeptide encoded by the DNA segments. 
     
     
         6 . A cultured cell comprising:
 a first expression vector comprising:
 a) a first transcription promoter; 
 b) a first DNA segment encoding a first cytokine receptor polypeptide comprising amino acid residues 20-649 of SEQ TD NO:109; and 
 c) a first transcription terminator; and 
   a second expression vector comprising:
 a) a second transcription promoter; 
 b) a second DNA segment encoding a second cytokine receptor polypeptide comprising amino acid residues 28-979 of SEQ ID NO:7; and 
 c) a second transcription terminator; 
   wherein the first cytokine receptor polypeptide and the second cytokine receptor polypeptide form a heterodimeric cytokine receptor; and   wherein the heterodimeric cytokine receptor binds to a ligand comprising amino acid residues 27-164 of SEQ ID NO:2.   
     
     
         7 . A method of producing a heterodimeric cytokine receptor comprising:
 culturing a cell according to  claim 4 ; and   isolating the heterodimeric cytokine receptor produced by the cell.   
     
     
         8 . A method of producing a heterodimeric cytokine receptor comprising:
 culturing a cell according to  claim 5 ; and   isolating the heterodimeric cytokine receptor produced by the cell.   
     
     
         9 . A method of producing a heterodimeric cytokine receptor comprising:
 culturing a cell according to  claim 6 ; and   isolating the heterodimeric cytokine receptor produced by the cell.