Adipose Stromal Stem Cells For Tissue And Vascular Modification

Abstract

Methods are provided for promoting angiogenesis in a mammal, such methods including the administration of therapeutic quantity of adipose-derived stromal cells to a mammal such that therapeutic angiogenesis occurs.

1 . A method for promoting angiogenesis in a subject mammal, comprising administration of therapeutic quantity of adipose-derived stromal cells to the subject mammal, such that therapeutic angiogenesis occurs. 
     
     
         2 . The method of  claim 1 , wherein said adipose-derived stromal cells are administered via a method selected from the group consisting of retrograde coronary venous delivery, retrograde delivery into other tissues, direct microinjection into target tissue, intra-arterial infusion via a catheter, intra-coronary infusion via a catheter, and systemic intravenous administration. 
     
     
         3 . The method of  claim 1 , wherein angiogenesis is promoted in cardiac tissue and said cells are administered via retrograde coronary venous delivery. 
     
     
         4 . The method of  claim 3 , wherein retrograde coronary venous delivery is conducted at pressures ranging from 30-400 mm Hg. 
     
     
         5 . The method of  claim 4 , wherein retrograde coronary venous delivery is conducted at pressures ranging from 50-150 mm Hg. 
     
     
         6 . The method of  claim 4 , wherein cells so delivered enter the interstitium. 
     
     
         7 . The method of  claim 1 , wherein said cells are administered in a biologically compatible medium. 
     
     
         8 . The method of  claim 7 , wherein said medium further comprises a viscosity-increasing carrier. 
     
     
         9 . The method of  claim 1 , wherein angiogenesis is promoted in ischemic tissue. 
     
     
         10 . The method of  claim 1 , further comprising:
 a) performing adipose tissue resection or suction on a donor mammal;   b) dissecting tissue obtained from said tissue resection or suction and dissociating said tissue into a cell suspension;   c) removing adipocytes from said cell suspension;   d) culturing the adipocyte-depleted cell suspension in a growth medium; and   e) isolating the adipose derived stromal cells.   
     
     
         11 . The method of  claim 10 , wherein the donor mammal is the subject mammal. 
     
     
         12 . The method of  claim 11 , wherein said mammal is a human patient. 
     
     
         13 . The method of  claim 10 , wherein the growth medium is EGM-2-MV. 
     
     
         14 . The method of  claim 13 , wherein the adipose derived stromal cells secrete vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and granulocyte-colony stimulating factor (G-CSF). 
     
     
         15 . The method of  claim 10 , further comprising the step, subsequent to step d) and prior to step e), of culturing the cell suspension in a growth-factor free basal medium. 
     
     
         16 . The method of  claim 15 , wherein the growth-factor free basal medium is EBM 2.