Oligonucleotide sequences that identify species of animal

Abstract

The present invention provides a method for identifying animal species, said method comprises a step of amplifying a DNA fragment by PCR using a DNA in a sample as a template and animal-specific DNA sequences as a primer pair, wherein the animal-specific DNA sequences are derived from a ATP synthase subunit 8 gene or a region proximal thereto of a mitochondrial genome; and a step of detecting the amplified DNA fragment.

1 . A primer pair for detection of a mammal-specific DNA, the primer pair being a combination of the DNA sequence of SEQ ID NO: 1 and the DNA sequence of SEQ ID NO: 2. 
     
     
         2 . The primer pair of  claim 1 , the mammal is selected from the group consisting of cattle, sheep, goat, deer, pig, horse, rabbit, and whale. 
     
     
         3 . (canceled) 
     
     
         4 . (canceled) 
     
     
         5 . (canceled) 
     
     
         6 . A primer pair for detection of a pig-specific DNA, the primer pair being a combination of the DNA sequence of SEQ ID NO: 17 and the DNA sequence of SEQ ID NO: 19, or a combination of the DNA sequence of SEQ ID NO: 18 and the DNA sequence of SEQ ID NO: 22. 
     
     
         7 . A kit for detecting an animal-derived component present in a sample comprising: at least one primer pair according to  claim 1 . 
     
     
         8 . The kit of  claim 7 , further comprising a primer pair for detection of a plant-specific DNA, the primer pair being a combination of the DNA sequence of SEQ ID NO: 42 and the DNA sequence of SEQ ID NO: 43.