Luminescence measurement method and luminescence measurement system

Abstract

Disclosed is a luminescence measuring method which can produce a luminous intensity depending on the amount of a substance to be measured even when the substance occurs in a biological sample in an amount equal to or more than a given amount, and which can achieve quantitative measurement. The method is characterized by includes preparing a biological sample containing a luminescence-associated protein which is can react with a substance occurring in the biological sample in amount equal to or more than a given amount and which has a Km value equal to or higher than a predetermined value so that the luminous intensity can be quantified depending on the amount of the substance, measuring the luminescence intensity emitted from the biological sample, and outputting a result of the measurement on a regions and/or part of the biological sample.

1 . A luminescence measuring method for measuring luminescence emitted from a biological sample, the method comprising:
 preparing a biological sample containing a luminescence-associated protein which is capable of reacting with a substance existing more than a prescribed quantity in the biological sample, the protein having a Km value which is higher than a prescribed value which enables to quantitatively measure a luminescence intensity in dependence with the substance;   measuring the luminescence intensity emitted from the biological sample prepared in above-described preparing step; and   outputting a measured result obtained from each of regions and/or sites of the biological sample.   
     
     
         2 . The method according to  claim 1 , wherein the substance is ATP;
 the luminescence-associated protein is luciferase; and the Km value is not less than 364.   
     
     
         3 . The method according to  claim 2 , wherein the luciferase is Yaeyama Hime firefly-originated luciferase to be created based on the DNA sequence of Sequence No. 1. 
     
     
         4 . The method according to  claim 1 , wherein the step of measurement includes a step of picking up a luminescence image based on the biological luminescence of the biological sample including a plurality of cells. 
     
     
         5 . The method according to  claim 4 , wherein the step of measurement includes a step of measuring the luminescence intensity of each of the cells. 
     
     
         6 . The method according to  claim 1 , wherein the step of preparation comprises a step of preparing the biological sample by making use of a plurality of luminescence-associated proteins differing in the Km value from each other. 
     
     
         7 . The method according to  claim 6 , wherein the step of measurement is performed depending on the Km value. 
     
     
         8 . The method according to  claim 7 , wherein the step of output is performed depending on the Km value.