Isolated genomic polynucleotide fragments that encode human lipoprotein-associated phospholipase A2

Abstract

The invention is directed to isolated genomic polynucleotide fragments that encode human lipoprotein-associated phospholipase A2, vectors and hosts containing the fragment and fragments hybridizing to noncoding regions as well as antisense oligonucleotides to these fragments. The invention is further directed to methods of using these fragments to obtain human lipoprotein-associated phospholipase A2 and to diagnose, treat, prevent and/or ameliorate a pathological disorder.

1 . An isolated polynucleotide probe or primer, wherein said probe or primer is a fragment of a polynucleotide which is at least 99% identical to SEQ ID NO:2 and wherein said polynucleotide probe or primer comprises at least 20 contiguous nucleotides of SEQ ID NO:2 or its full complement which contains a transcription factor binding site at an AP1_C site at nucleotides 3731-3741, 4338-4348, 5013-5025, 15556-15566, 15596-15606, 19533-19543, 35142-35152, 38600-38610, 40256-40266, 41192-41202 or 41585-41595 of SEQ ID NO:2; an AP4_Q5 site at nucleotides 32929-32945or 38920-38936 of SEQ ID NO:2; a GATA1 — 04 site at nucleotides 25798-25810, 27827-27839, 28501-28513, 31629-31641, 32034-32046, 32169-32181, 33274-33286, 33538-33550or 41958-41970 of SEQ ID NO:2; a GATA1 — 06 site at nucleotides 30717-30729, 31464-31476, 35301-35313 or 41365-41377 of SEQ ID NO:2; a GATA2 — 02 site at nucleotides 3486-3498, 3609-3621, 9107-9119, 10637-10659, 18349-18361, 20858-20870, 32236-32248, 34609-34621, 35665-35677, 36781-36793 or 42161-42173 of SEQ ID NO:2; a GATA3 — 02 site at nucleotides 24794-24806, 25834-25846, 31071-31083, 31973-31985 or 35551-35563 of SEQ ID NO:2; a GATA_C site at nucleotides 343-355, 650-662;3370-3382, 4507-4519, 5160-5172, 7500-7512, 8422-8434, 10595-10607, 17905-17917, 22600-22612, 24775-24797, 27763-27775, 30027-30039, 30541-30553, 33695-33707, 33935-33947 or 41929-41941 of SEQ ID NO:2; a MZF1 — 01 site at nucleotides 2907-2915, 4766-4774;10854-10862, 11216-11224, 12675-12683, 15186-15194, 27471-27479, 28548-28556 or 33107-33115 of SEQ ID NO:2; an NFAT_Q6 site at nucleotides 1557-1575, 3717-3735, 3779-3797, 4621-4639, 5588-5606, 7380-7398, 9288-9306, 11523-11541, 12221-12239, 13103-13121, 13236-13254, 14475-14793, 16579-16597, 19812-19830, 21660-21678, 26158-26176, 27106-27124, 27872-27890, 28104-28122, 29107-29125, 32393-32411, 32816-32834, 33285-33303, 34446-35564 or 40661-40679 of SEQ ID NO:2; a NKX25 — 01 site at nucleotides 4402-4416, 8109-8123 or 33218-33232; a 58 — 01 site at nucleotides 2836-2848, 3390-3402, 3393-3405, 3872-3884, 3875-3887, 4227-4239, 12123-12137, 13569-13583, 15144-151458, 17041-17055, 21032-21046 or 28959-28973 of SEQ ID NO:2; a S8 — 01 site at nucleotides 12775-12787, 13388-13400, 14000-14012, 18248-18260, 25893-25905, 25896-25908, 25948-25960, 27231-27243 or 27497-27509 of SEQ ID NO:2; a SOX5 — 01 site at nucleotides 42438-42454 of SEQ ID NO:2; a TATA_C site at nucleotides 25236-25242, 25245-25261, or 41494-41510 of SEQ ID NO:2 or a TCF11 — 01 site at nucleotides 2093-2099, 10078-10084, 10792-10798, 14200-14206, 15162-15168, 28408-28414, 32852-32858, 34893-34899, 38599-38905 or 41773-41779 of SEQ ID NO:2. 
     
     
         2 . A method for isolating the polynucleotide of  claim 1  comprising
 (a) isolating genomic DNA from a sample; 
 (b) providing primers, probes and optionally polymerase and 
 (c) incubating (a) and (b) under conditions promoting the isolation of said nucleic acid molecule. 
 
     
     
         3 . A kit comprising the polynucleotide of  claim 1 . 
     
     
         4 . The polynucleotide according to  claim 1 , wherein said polynucleotide is an RNA or DNA.